基于冷冻电镜技术的生物大分子机器近原子分辨率结构解析

In the recently years, the 3D structure reconstruction of biomacromolecules by cryo electron microscopy (cryoEM) is fast growing in the Structural Biology field and is making breakthroughs toward the atomic resolution. In this proposal, we will investigate the structures of an icosahedral virus in different states of its life cycle by 3D cryoEM reconstruction at near-atomic resolution, and reveal the structural and functional changes of virus at different stages and the underlying molecular mechanism.We will also reconstruct the 3D structure of chromatin remodeling complexes with low or non- symmetry at high or near-atomic resolution, and analyze the factors which may contribute to the reconstruction resolution by cryoEM.

近年来，冷冻电镜（cryoEM）三维重构方法在结构生物学领域发展迅速并正在向原子分辨率方向取得重要突破。本项目以冷冻电镜三维重构为主要手段，系统研究二十面体对称病毒在生命周期中不同阶段和状态的原子分辨率结构模型，对病毒生命周期中的结构和功能变化以及分子调控机制进行深入分析。同时，以染色质重塑相关复合物为研究对象，进行具有较低对称性或不具有对称性的大分子复合体的近原子分辨率或较高分辨率的冷冻电镜三维结构重构，系统分析各种因素对冷冻电镜三维重构分辨率的影响。