基于拉曼光谱技术的精液质量研究

In this project, Raman spectroscopy was applied for the investigation of human semen quality. Conventional Raman and SERS spectra of seminal plasma were first obtained from fertile and infertile male, and the biochemical differences in seminal plasma between these two groups were analyzed and compared by assigning Raman spectra and extracting Raman characteristic bands; then the concentrations of the two important metabolites such as citric acid and fructose were quantitatively analyzed with the combination of partial least square method and the Raman spectra which obtained from ultrafiltrate of seminal plasma with proteins removed. In addition, micro-Raman spectroscopy was utilized to preliminarily study the immobilized and live spermatozoa, particularly for the assessment of DNA integrity in spermatozoa head. We have carried out some effective preliminary work and published some papers in international journals. We anticipate that further in-depth study will provide a rapid and non-destructive method for semen quality assessment and the screening of high-quality spermatozoa. This research will help to solve the problem of the declines in human fertility and has great academic and social value.

本项目采用拉曼光谱技术研究人体精液。首先，获取正常和不育男性精浆的常规拉曼光谱与SERS光谱，通过对光谱数据的谱峰归属、特征谱峰的提取，分析比较精浆中生化物质的差异；测量滤除蛋白分子后的精浆超滤液的拉曼光谱，利用偏最小二乘法，定量分析精浆中两种重要代谢物柠檬酸和果糖含量；其次，采用显微拉曼光谱技术研究体外固定精子与活体精子，包括人活体精子头部DNA的拉曼光谱。申请者已开展卓有成效的前期研究，已连续在国际重要刊物发表论文。本项目研究的进一步深入有可能为男性精液质量的检测，包括精浆质量检测与优质精子筛选提供一种快速、无损的新方法。本项目将有助于协助解决人类生殖能力下降的问题，具有重要的学术意义和社会价值。

本项目采用拉曼光谱技术研究人体精液。首先，获取正常和不育男性精浆的常规拉曼光谱与SERS光谱，通过对光谱数据的谱峰归属、特征谱峰的提取，分析比较精浆中生化物质的差异；测量滤除蛋白分子后的精浆超滤液的拉曼光谱，利用偏最小二乘法，定量分析精浆中两种重要代谢物柠檬酸和果糖含量；其次，结合显微拉曼光谱技术和图像处理方法获得精子形态的自动识别和判断，以及精子头部DNA的拉曼光谱刻画，并初步获得可评估精子DNA完整性的光谱特征数据。本项目还成功开展了活体精子拉曼光谱的初步测量，项目的进一步深入研究有望为男性精液质量的检测与优质精子筛选提供一种快速、无损的新方法。

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