有序金属纳米结构-阵列纳米通道复合器件增强光电生物分析

Novel methods, technologies, and devices will certainly provide important scientific and technologic supports for understanding the chemistry nature of life. In this project, array nanochannels and ordered metal nanostructures will be coupled to form a hybrid device. In this device, the tunable localized surface plasmon resonance (LSPR) of the ordered metal nanostructures generates enhanced surface electric fields, which in turn enhances the Raman scattering and electrochemical signals. Combining the unique mass transport properties and the ionic current rectification (ICR) effect of the hybrid device, the designed method enables label-free and sensitive detection of biomolecular recognition events with enhanced electrochemical and Raman scattering spectroscopic signals as the transducer. The influence of nanoparticles size, surface property, and assembly modes on the morphology, nanogap and LSPR property of the ordered structure will be investigated systematically. Then the mechanism of LSPR enhanced electrochemical and optical performance will be revealed. The relationship between biomolecular recognition and ICR characteristics will be studied, based on which a series of biomolecular recognition events will be investigated. Using the enhanced Raman scattering spectroscopies which reveal the structure/conformation and electric properties of the biomolecules, the reaction kinetics of biomolecular recognition events will be studied. Realization of the project promises the construction of a practical platform for bioanalysis with high sensitivity, excellent selectivity and good stability, and will promote the development of nanofluidics in the field of bioanalysis.

生物分析新方法、新技术、新器件为人类认识生命的化学本质提供重要的科学和技术支撑。本项目拟将阵列纳米通道和金属纳米粒子有序结构相结合，利用纳米粒子表面等离子体共振 (LSPR) 增强光、电性质，阵列纳米通道的物质输运性质，以及纳流控器件的离子整流特性，发展有序金属纳米结构-阵列纳米通道复合器件增强光电生物分析新方法。项目将系统研究金属纳米粒子大小、表面性质、组装方式等对纳米有序结构形貌、间隙，及其LSPR性质的影响，揭示有序金属纳米结构对电化学、表面增强拉曼的作用规律和增强机制；研究复合器件物质传输性质与生物分子识别之间的关联，实现生物分子高灵敏、无标记识别检测；同时结合光谱变化对生物分子结构、形态、电性等信息进行原位分析，揭示生物分子识别反应的动力学机制。研究成果将为发展高稳定性、高选择性、高灵敏生物分子识别器件提供理论基础和研究范例，同时为纳流控技术在生物分析中的应用起到重要的推动作用。