基于三维近场生物电纺技术构建移行结构化肌腱复合物

As the "stage" of cell activity, biomaterials scaffolds have shifted their focus from simple physical scaffolds to biological ones. However, functional scaffolds with induced tendon differentiation are still the vacancy of tendon tissue engineering. This project is to study the possibility of creation of core-shell structure aligned biomimetic fibers, which based on three dimensional printing and near field electrostatic spinning technique, combine with PHA materials, seed cells and type I collagen, to investigate whether it can promote ligament-bone repair. This technique can not only regulate cell differentiation for regeneration of anisotropic tissues, but also provide a platform to explore the underlying mechanisms that govern the cell behavior. Highly-aligned biomimetic fibers are of great values in constructing anisotropic tissues and pinpointing the underlying mechanisms of anisotropic tissue regeneration.

生物材料支架作为细胞活动的“舞台”，其研究重点已从简单的物理学支架功能转移到生物学诱导功能，而具有诱导腱系分化的功能性生物支架至今仍然是肌腱组织工程研究的空窗。本课题利用3D打印技术精确的个性化支架构建能力，辅以近场静电纺丝技术仿生的纳微米级别的微观支架构建能力，将聚羟基脂肪酸酯系列材料、I型胶原蛋白、骨髓间充质干细胞进行三维同轴生物电纺，研究结合有形态仿生和生物化学信号修饰的芯壳取向复合纤维支架在体外构建组织工程化肌腱时的协同诱导效应及作用机制。该三维生物电纺技术不仅为诱导特定的结构特异性组织的细胞分化提供了新的思路和方法，而且为揭示仿生取向纤维的作用机理提供了理想的研究平台，在实现不同结构特异性组织的仿生构建及组织再生机制研究中具有重要应用价值。