纳米硒-多糖载体的构建及其对LPS诱导巨噬细胞损伤保护机制的构效关系研究

Nano-Se (SeNPs) have good advantages in bioavailability, high biological activity and low toxicity, but the bioactivities mechanism and application of SeNPs in food are limited due to the preperation of stable SeNPs solution is difficult. Our previous studies showed that this problem can be solved by the polyhydroxy and high specific surface area polysaccharides, which is one of the most abundant water-soluble dietary fiber. Meanwhile, our preliminary experiment results showed SeNPs obviously inhibited the NO production of macrophage induced by lipolysaccharides(LPS), and the mechanisms and structure-activity relationships need to be further studied. We predict that SeNPs can regulate the signal pathways of the iNOS of macrophages induced by LPS. This project plans to study the inhibition mechanism of SeNPs on the NO production of macrophages induced by LPS, and the relationship between the bioactivites and the size and morphrloge of SeNPs, and the secondary structure of SPS-SeNPs also will be discussed. Furthermore, this project can establish the theoretical basis of the construction of SeNPS-plysaccharides, meanwhile, it can also accumulate scientific data for the application of selenium functional factor.

纳米硒（SeNPs）具有良好的生物利用度、高生物活性和低毒性的优点。由于SeNPs的稳定溶液制备比较困难，限制了其活性分子机制研究及其在食品中的应用。利用前期研究结果，基于可溶性多糖膳食纤维的多羟基和高比表面积特性，有效制备了SeNPs稳定溶液，初步研究表明，SeNPs可有效抑制LPS诱导的巨噬细胞损伤作用，而其分子机制及构效关系尚未明确，难以对产业化开发提供科学依据和理论方法。我们推测: SeNPs可能通过调控LPS激活巨噬细胞过量表达iNOS的相关信号通路而发挥作用。本项目拟通过体内体外实验对其抑制LPS诱导巨噬细胞过量产生NO的机制进行研究；分析纳米硒的形貌尺寸以及纳米硒-多糖载体的二级结构与活性之间的构效关系，探究其活性分子机制；该项目的开展不仅可为纳米硒-多糖载体的构建奠定理论基础，还可为硒功能因子在食品中的应用积累一定的科学数据。