Pickering高内相乳液模板构建双重识别自支撑多孔印迹膜选择性分离纯化木犀草素的研究

The component of extracts from peanut shell is complex, which requires further separation and purification to remove impurities in order to promote luteolin's application in health-care products, anticancer, antibacterial, and other clinical treatment. This project intends to utilize molecularly imprinted polymers modified graphene oxide (GO) as stabilizer for the construction of Pickering high internal phase emulsions (HIPE), and substituted boronic acid monomer and non-covalent imprinted monomer will be introduced into their continuous phase. Then free-standing molecularly imprinted porous membrane with double recognition abilities, fabricated via thermal initiated imprinting polymerization of Pickering HIPE template in an optimized mold, will be adopted for the selective separation and purification of luteolin from extracts. This research is aimed at selective separation and purification of luteolin by free-standing molecularly imprinted porous membrane with double recognition abilities, and the membrane preparation method and performance optimization are recognized as critical control point. Through adjusting membrane thickness, controlling over the pore structure, and optimizing recognition ability of imprinted binding sites, the purity and yield of luteolin will be effectively elevated. On this basis, the adjustments and controls law of preparation of free-standing molecularly imprinted porous membrane with double recognition abilities will be elucidated. The mechanism and synergetic effect of imprinted binding sites with double recognition abilities for the enhancement of selectivity will also be investigated. Moreover, the structure-activity relationship of the free-standing molecularly imprinted porous membrane with double recognition abilities will be revealed, and the possible recognition and mass transfer mechanism will be described. Finally, a new strategy for the selective separation and purification of luteolin from peanut shell extracts will be established.

花生壳中木犀草素提取液成分复杂，需进一步分离纯化才能应用于保健品和抗癌、抑菌等临床治疗环节。本项目拟以印迹聚合物修饰的氧化石墨烯(GO)为稳定粒子构建Pickering高内相乳液(HIPE)模板，连续相中同时引入取代硼酸单体和非共价印迹单体，转入优化的模具中热引发印迹聚合制备双重识别自支撑多孔印迹膜，并用于选择性分离纯化花生壳提取液中的木犀草素。研究中以自支撑多孔印迹膜选择性分离纯化木犀草素为主线，以膜的制备方法和性能优化为关键控制点，通过膜厚度可控调节、孔结构有效控制和印迹位点识别程度增强提高木犀草素的纯度和得率。在此基础上，阐明Pickering HIPE模板构建双重识别自支撑多孔印迹膜及其微结构调控的规律，探究双重识别印迹位点协同增强膜识别能力的机理；揭示双重识别自支撑多孔印迹膜的构效关系，解释可能的识别与传质机制，建立选择性分离纯化花生壳提取液中木犀草素的新方法。