CD147在LPS诱导小胶质细胞活化中的作用

The mechanism in LPS-induced neural inflammatory injuries is a hot point of neurotoxicology. The activation of microglia plays a critical role in LPS-induced neurotoxicities. Matrix metalloproteinases (MMPs) are an important group of regulatory factors for mciroglial activation, which are regulated by CD147, a key matrix metalloproteinase inducer. CD147 can induce inflammatory reactions through the induction of MMPs. But there is no existing evidence whether CD147 plays a role in the activation of microglia. Our preliminary results showed that LPS could induce the expression of CD147, suggesting a potential role in LPS-induced microglial activation. In this study, both in vivo and in vitro models will be established. Then varied technical means will be employed to investigate the role of CD147 in LPS-induced microglial activation. Then further work will be carried out to elucidate the possible mechanisms involved from an angle of MMPs and autophagy. Our data may shed new light on clues and theoretical foundations for the elucidation of LPS-induced inflammatory injuries in central nervous system.

LPS诱导神经炎性损伤的机制是神经毒理学的一个热点。小胶质细胞活化在LPS诱导的神经毒性中发挥关键作用。基质金属蛋白酶（matrix metalloproteinases，MMPs）是调控小胶质细胞活化的重要因子，而CD147又是关键的MMPs诱导因子，可通过诱导MMPs进而诱导炎症反应，但其是否参与了小胶质细胞活化的调控尚无报道。我们前期的研究结果显示，LPS可诱导小胶质细胞CD147的表达，提示其可能参与了LPS诱导的小胶质细胞活化。本研究在建立体内外模型的基础上，通过多种技术手段，探讨CD147在LPS诱导小胶质细胞活化中的作用，并从MMPs和自噬角度初步探讨其中的机制，从而为揭示LPS诱导中枢神经系统炎症损伤的机制提供新的理论依据。