Confocal imaging plate reader for advanced cell biology applications

适用于高级细胞生物学应用的共焦成像读板机

• 批准号: RTI-2022-00404
• 负责人: Khursigara, Cezar
• 金额: $ 10.93万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=761151
• 关键词: Confocal; imaging; plate; reader; advanced; Confocal; imaging; plate; reader; advanced

Cost-effective high throughput imaging is the cornerstone of modern cell biology laboratories. However, traditional fluorescence imaging plate readers lack the ability to resolve intricate cellular details in 3D, while confocal microscopes that function to screen samples in high throughput are prohibitively expensive. This application seeks to fund a Cytation C10 Spinning Disk Confocal Imaging Plate Reader to support advanced cell biology initiatives from microbiomes to embryos. The Cytation C10 is currently the only confocal imaging plate reader available and combines automated spinning disk confocal microscopy, widefield microscopy and multi-mode detection in one compact system. This combination of imaging modalities will enable our research groups to image and quantify complex cellular information to obtain meaningful results faster. The NSERC-funded research of the applicants focuses on cutting-edge aspects of cell biology. The applicants share a common challenge of obtaining high-resolution image datasets of complex cellular systems in 3D, rapidly while yielding quantitative information. The research themes include rapid imaging of morphology and protein localization in bacterial cells, visualizing outputs of chemical genetic screens, dissecting signalling and molecular pathways involved in bacterial-host interactions, and studying endocrine and neuronal development. This infrastructure is urgently needed as the lack of access to robust, high throughput multi-mode imaging has severely limited the scope of their research programs. The research projects of HQP are currently limited in scope and hampered by the need to access equipment at distant sites, or the use of inconsistent and time-consuming manual microscopy methods. The ability to perform large-scale screens rapidly and accurately is thus critically needed and will enable HQP to ask more ambitious research questions. The team of applicants and their HQP represent a range of backgrounds and reflect the diversity of Canadian society today.

具有成本效益的高通量成像是现代细胞生物学实验室的基石。但是，传统的荧光成像读取器缺乏解决3D中复杂的细胞细节的能力，而在高通量中筛选样品的共聚焦显微镜非常昂贵。该应用程序旨在资助Cytation C10旋转磁盘共聚焦成像读板读取器，以支持从微生物到胚胎的先进细胞生物学计划。 Cytation C10当前是唯一可用的共聚焦成像读取器，并结合了一个紧凑型系统中自动旋转磁盘共聚焦显微镜，广场显微镜和多模式检测。成像方式的这种组合将使我们的研究组能够形象和量化复杂的细胞信息，以更快地获得有意义的结果。 NSERC资助的申请人研究的重点是细胞生物学的尖端方面。申请人在3D中获得复杂细胞系统的高分辨率图像数据集的共同挑战，同时得出定量信息。研究主题包括对细菌细胞中形态学和蛋白质定位的快速成像，可视化化学遗传筛选的输出，剖析信号传导以及参与细菌宿主相互作用的分子途径以及研究内分泌和神经元发育。由于缺乏可靠，高吞吐量的多模式成像的访问，因此迫切需要这种基础设施，严重限制了其研究计划的范围。 HQP的研究项目目前在范围上受到限制，并受到访问遥远站点或使用不一致且耗时的手动显微镜方法的需求的阻碍。因此，急需快速准确地进行大规模筛选的能力是至关重要的，并且将使HQP能够提出更多雄心勃勃的研究问题。申请人及其HQP团队代表了一系列背景，并反映了当今加拿大社会的多样性。