Democratization of Mass Spectrometry Through Simplified Sample Preparation/Ionization for High Impact Applications

通过简化样品制备/电离实现高影响应用的质谱分析大众化

• 批准号: RGPIN-2022-03833
• 负责人: Oleschuk, Richard
• 金额: $ 5.61万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=748032
• 关键词: Democratization; Mass; Spectrometry; Through; Simplified

Chemical analysis forms the basis for discovery in a wide variety of disciplines (e.g. environmental, medical and "omics" related research areas). The development of analysis systems that are faster, require less sample, and offer increased sensitivity and compatibility with a variety of matrices are critical to probing e.g. biological and chemical systems. My research program proposal leverages our expertise in microfluidics, mass spectrometry, and separation science and will focus on novel methods to enhance, simplify and ruggedise both sample preparation and introduction for mass spectrometry, and apply them to high impact applications. The proposed program will involve four research thrusts. The first will focus on the use of hydrophobic/hydrophilic patterned surfaces that self-wet to quickly yield micro-droplet arrays to carry out rapid sample preparation (tissue imaging, liquid chromatographic archiving and preconcentration). The second will focus on enhancing a novel mass spectrometric ambient ionization interface (liquid micro-junction surface sampling probe, LMJ-SSP) to rapidly sample the broader chemical space of complex biological samples using a "gradient at the tip approach". Both thrusts one and two seek to further democratise mass spectrometry, taking it from specialised, well-trained operators to more routine field use in public health, and hospital settings.  The third thrust will utilise the LMJ SSP to characterise biofilms formed on different relevant surfaces. Biofilms protect the microbial community from external pressures, including antibiotics, immune cells and drinking water disinfection. Trainees will work in the Drinking Water Distribution Laboratory (DWDL) at Queen's and at the newly minted Centre for Health Innovation (Kingston General Hospital) to assess biofilm growth on relevant surfaces. Contrastingly, the microbes that make up biofilms produce a number of bioactive compounds for mutualistic benefit and/or as a survival tool. In collaboration with Dr. Avena Ross's group at Queen's will be using novel MS tools to examine different species of Psuedoalteromonas grown on different scaffolds to search for novel biosynthetic compounds. The fourth thrust  involves working with Dr. Natalie Agar, Harvard School of Medicine, USA (Cancer) and Dr. Sabeth Verpoorte, Groningen, The Netherlands (Liver) and the Molecular Navigation Group at Queen's to develop LMJ-SSP and) as a rapid tissue imaging tool for intra-operative assessment of tumour margins and liver function. Equity, Diversity, and Inclusion will continue to be a priority of my research program. HQP will be required to participate in formal EDI training programs organised through the University Human Rights and Equity Office. HQP will benefit from working at discipline interfaces where researchers will be trained on tissue preparation, chemical/biochemical safety, and state-of-the-art electrochemical, mass spectrometric, microbiological and microfabrication techniques.

化学分析构成了学科的基础（例如环境，医学和相关”）的开发速度更快，需要更少样本并提高了与各种矩阵的敏感性和兼容性，这对于探测生物和化学系统至关重要。光谱法，并将重点放在新的方法上，以简化质谱的样本制备，并将其应用于高含义。进行快速样品制备的阵列（液体色谱归档和预浓缩）。推力将利用LMJ SSP来表征不同相关表面上的生物膜，包括外部压力，包括抗生素，免疫细胞和饮酒，在皇后区和新的Mintre上工作。健康创新（金斯敦综合医院评估相关表面的生物膜。组成生物膜的微生物产生了一种非属性化合物，以实现Mutul Istic益处和/或如Surlvival工具。在不同的脚手架上检查不同脚手架的伪有抗细胞动物的工具，以寻找与美国哈佛大学的Natalie Agar博士（癌症）和Sabeth Verpoorte博士一起工作的第四个推力。皇后区的分子导航是对肿瘤和肝功能的快速组织成像工具的快速术内评估。参与的EDI培训CAVERUGH CAAN权利和公平率将在纪律界面上工作。