Stress-induced flowering response to abiotic stimuli in sustainable bio-systems

可持续生物系统中应激诱导的开花对非生物刺激的反应

• 批准号: RGPIN-2019-04931
• 负责人: Gravel, Valérie
• 金额: $ 2.04万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=743305
• 关键词: Stress; induced; flowering; response; abiotic

Flowering in horticultural crops is the result of physiological, chemical and molecular changes. Regulation of these is done either autonomously (e.g. genotype) or through the effect of environmental factors (e.g. photoperiod or temperature). Flower bud induction remains a limiting factor in intensive and sustainable fruit production systems. Recently, stress-induced flowering has received increased attention as a potential way to manipulate fruit set and the overall quality of produced fruits due to the plant response to stress. Using Fragaria spp. as a model plant, my research program is based on the general hypothesis that fruit set and quality composition can be enhanced through manipulation of stress-induced flowering in day-neutral species. The long-term objective is to elucidate the biochemical and genetic mechanisms that elicit and regulate stress-induced flowering in Fragaria spp. and evaluate the subsequent impact on berry quality. My research team and I will first of all 1) characterize the physiological flowering response of day-neutral Fragaria spp. to individual and combined abiotic stimuli. We will then 2) elucidate the role of secondary metabolites as signal molecules regulating flower bud induction in Fragaria spp. in response to abiotic stimuli. Finally, we will 3) characterize the regulation of key genes involved in stress-induced flowering in Fragaria vesca. These objectives will be achieved through a well-balanced combination of classic phenotypic assessment of plant development and state-of-the-art strategies for transcriptomic and metabolite analysis (RNA-seq and HPLC). The knowledge generated by the three proposed objectives will provide a clear understanding of the regulation of stress-induced flowering in Fragaria spp. and will highlight the potential use of abiotic stimuli manipulation to enhance flowering and berry quality. This will benefit the Canadian strawberry industry by providing new practices to improve fruit production as well as consumers by increasing availability of locally-produced berries. My research answers a need to pursue discoveries in the field of sustainable development within the biofood industry to ensure long-term health benefits for consumers by promoting improved resource use efficiency in high-quality fruit production, which directly addresses one of the seven priority areas targeted by the Canadian government. My research program will also have a strong socio-economic impact through the unique multidisciplinary training platform for Ph.D. (2), M.Sc. (2) and undergraduate (5) students, allowing them to gain expertise and a complete skill set in a sector characterized by a high demand in trained scientists.

园艺作物中的开花是生理，化学和分子变化的结果。这些调节是自主进行的（例如基因型）或通过环境因素（例如光周期或温度）的影响。诱导花芽仍然是密集和可持续水果生产系统的限制因素。最近，由于植物对压力的反应，压力引起的开花受到了操纵水果集的潜在方法的越来越多的潜在方法。使用Fragaria spp。作为模型植物，我的研究计划基于以下一般假设：通过操纵白天中性物种中压力诱导的开花，可以增强水果集和质量成分。长期目的是阐明引起和调节Fragaria spp中应激诱导的开花的生化和遗传机制。并评估随后对浆果质量的影响。我和我的研究团队首先将所有1）表征日常的fragaria spp的生理开花反应。对个体和合并的非生物刺激。然后，我们将2）阐明二级代谢产物作为调节Fragaria spp中花蕾诱导的信号分子的作用。响应非生物刺激。最后，我们将3）表征涉及压力诱导的Fragaria Vesca开花的关键基因的调节。这些目标将通过对植物发育以及转录组和代谢物分析的最新策略（RNA-SEQ和HPLC）的经典表型评估的均衡结合来实现。提出的三个目标产生的知识将清楚地了解Fragaria spp中压力引起的开花的调节。并将强调非生物刺激操纵的潜在用法来增强开花和浆果质量。这将通过提供新的实践来改善水果产量以及增加当地生产的浆果的可用性，从而使加拿大草莓行业受益。我的研究回答了在生物食品行业内可持续发展领域追求发现的需求，以通过提高改善资源利用效率的高质量水果生产，以确保消费者的长期健康益处，该效率直接解决了加拿大政府针对的七个优先领域之一。我的研究计划还将通过博士学位的独特多学科培训平台产生强烈的社会经济影响。 （2），硕士（2）和本科生（5）学生，使他们能够获得专业知识和完整的技能，其特征是受过训练的科学家的需求量很高。