Towards Functional Peptide-Based Supramolecular Structures

迈向基于功能性肽的超分子结构

• 批准号: RGPIN-2017-04834
• 负责人: Voyer, Normand
• 金额: $ 2.55万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=711102
• 关键词: Towards; Functional; Peptide; Based; Supramolecular

Towards Functional Peptide-Based Supramolecular Structures Inspired by natural sophisticated proteic systems, our research program aims at developing functional supramolecular structures exploiting polypeptide as molecular framework. For the next grant period, we propose to devote our efforts into four areas: 1. Use artificial ion channels as sensing devices We will pursue our development of crown ether peptide-based ion channels towards sensing devices. Several biological recognition elements will be introduced at the N-terminal position to study and eventually to detect the presence of specific biomarkers and cells. 2. Preparing fluorinated channels and nanopores We will synthesize fluorinated helical peptides and study their self-assembly into novel supramolecular architectures driven by fluorine-fluorine interactions. Such superstructures will create fluor-lined channels and nanopores. We intend to study membrane ion transport properties of those fluorinated channels to understand the structural features essential for ion translocation. 3. Developing antimicrobial peptide nanostructures Towards novel and efficient antimicrobials against resistant bacteria, we have used a de novo approach based on a simple 14 residue peptide. We propose to engineer systematically this model peptide with specific functional groups and to study their antimicrobial activities towards important pathogens. Modifications at terminal positions and in the sequence will be evaluated with respect to their impact on bioactivity. 4. Biomimetic supramolecular catalysis in water To catalyze efficiently industrially relevant organic transformations in water, we propose to synthesize a variety of peptide structures that can acts as supramolecular catalysts, hence mimicking natural enzymes. We will investigate several peptidic systems; from small rigid cyclic peptides, to conformationally biased oligopeptides. Different transformations will be studied as model reactions. Factors influencing efficiency will be identified with the goal of delineating design principles for developing biomimetic catalysts for a huge variety of transformations in water.

朝向基于功能肽的超分子结构 受自然复杂蛋白系统的启发，我们的研究计划旨在开发功能性超分子结构，利用多肽作为分子框架。在下一个赠款期间，我们建议将我们的努力投入四个领域： 1。使用人工离子通道作为感应设备 我们将追求基于冠状肽的离子渠道的开发，用于传感设备。将在N端位置引入几种生物学识别元件，以最终检测特定的生物标志物和细胞的存在。 2。准备氟化通道和纳米孔 我们将合成氟化的螺旋肽，并将其自组装研究成由氟氟氟相互作用驱动的新型超分子体系结构。这样的上层建筑将创建荧光衬里的通道和纳米孔。我们打算研究这些氟化通道的膜离子传输特性，以了解离子易位必不可少的结构特征。 3。开发抗菌肽纳米结构 倾向于针对抗性细菌的新型抗菌剂，我们使用了基于简单的14个残基肽的从头方法。我们建议通过特定功能组系统地设计该模型肽，并研究其针对重要病原体的抗菌活性。将在末端位置和序列中的修改将其对生物活性的影响进行评估。 4。水中的仿生超分子催化 为了催化水中有效与工业相关的有机转化，我们建议合成各种可以充当超分子催化剂的肽结构，因此模仿天然酶。我们将研究几个肽系统。从小的刚性环状肽到构象有偏置的寡肽。将以模型反应研究不同的转换。影响效率的因素将被确定，目的是描述为开发仿生催化剂的设计原理，以用于水中的各种转化。