Evaluation of the possible potential of essential oils present in emulsion or under vapour against Covid-19

评估乳液或蒸气中精油对抗 Covid-19 的可能潜力

• 批准号: 554809-2020
• 负责人: Lacroix, Monique
• 金额: $ 3.64万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=705684
• 关键词: Evaluation; possible; potential; essential; oils

The essential oils (EOs) and their chemical constituents are known to be active against a wide range of viruses like herpes simplex virus type 1 (HSV 1) (Astani et al., 2010). The active compounds in essential oils are normally related to the presence of terpenes, especially monoterpenes and phenolic compounds which are the volatiles part in essential oils. Some reports on natural extracts and essential oils have demonstrated antiviral activity against coronaviruses to some extent in both emulsion phase and vapor phase (Mani et al., 2020). Since the new strain of coronavirus, Covid-19, is still not completely understood, it is not yet possible to find which EOs will offer the best level of protection. However, it is plausible to assume that some of the EOs and related terpenes are likely to offer a measurable level of defense in the same way that they do with other known viruses such as HSV 1. We have demonstrated in several studies that EOs in vapor or in solution can inhibit bacteria and fungus, and even virus i.e. norovirus (flavonoids from citrus extract) (Gobeil et al., 2020). The main objectives of the proposal are: (1) Evaluate the minimum inhibitory concentrations (MIC) and fractional inhibitory concentrations (checkerboard method) of EOs (FIC calculation: evaluation of the synergy between EOs) under emulsion form against Covid-19 using broth microdilution and checkerboard method; (2) Evaluate the MIC and FIC of EOs in vapor form against Covid-19 using a new broth volatilization checkerboard method; (3) Develop EOs formulation that could be applied under solution or vapor form on Covid-19. The results of the study will permit to develop anti Covid-19 formulations under the form of emulsion or for vapor application for diverse applications such as working or food surface or even for air decontamination.

已知精油 (EO) 及其化学成分对多种病毒具有活性，例如 1 型单纯疱疹病毒 (HSV 1)（Astani 等，2010）。精油中的活性化合物通常与萜烯的存在有关，特别是单萜和酚类化合物，它们是精油中的挥发性部分。一些关于天然提取物和精油的报告已经证明在乳液相和气相中都具有一定程度的针对冠状病毒的抗病毒活性（Mani et al., 2020）。由于新型冠状病毒 Covid-19 尚未完全了解，因此尚无法找到哪些 EO 能够提供最佳水平的保护。然而，可以合理地假设，一些 EO 和相关萜烯可能会提供可测量水平的防御，就像它们对其他已知病毒（例如 HSV 1）所做的那样。我们在几项研究中已经证明，蒸气中的 EO或在溶液中可以抑制细菌和真菌，甚至病毒，即诺如病毒（来自柑橘提取物的黄酮类化合物）（Gobeil 等人，2020）。该提案的主要目标是： (1) 使用肉汤微量稀释法评估乳液形式下 EO 对 Covid-19 的最低抑菌浓度 (MIC) 和分数抑菌浓度（棋盘法）（FIC 计算：评估 EO 之间的协同作用）和棋盘法； (2) 使用新的肉汤挥发棋盘法评估蒸气形式的 EO 对 Covid-19 的 MIC 和 FIC； (3) 开发可在溶液或蒸气形式下应用于 Covid-19 的 EO 配方。该研究的结果将允许开发乳液形式的抗 Covid-19 制剂，或用于多种应用的蒸汽应用，例如工作或食品表面，甚至空气净化。