Development of lab on a chip for rapid and accurate point of care detection of COVID-19

开发芯片实验室，用于快速、准确地检测 COVID-19

• 批准号: 552043-2020
• 负责人: Ahamed, Mohammed
• 金额: $ 3.64万
• 依托单位: University of Windsor
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=703303
• 关键词: Development; lab; chip; rapid; accurate

Until a vaccine is developed, the ability to reduce the transmission of COVID-19 is directly dependent on the capacity to test and isolating the infected. Currently, COVID-19 testing is performed in a sophisticated laboratory setup that requires time and sample routing to designated labs. Accurate, rapid, onsite and point of care (POC) detection has paramount importance not only in Canada but also worldwide for early intervention and infection control. Development of such a device will be highly impactful for our fight against COVID-19. Most of the COVID-19 test are based on quantitative reverse transcription polymerase chain reaction (qRT-PCR) of the virus RNA. PCR takes hours to reach a result and requires laboratory settings. Portable isothermal nucleic acid amplification and loop-mediated isothermal amplification (LAMP) systems are fast but not suitable for wide usage due to the higher cost. Alternately, antibody based POCs offer cost-effective but it can only detect whether an individual has been previously infected or not. These drawbacks in the current technologies are hindering the fight a contagious virus such as COVID-19, tests must be quick (minutes not hours), cost effective, portable and be highly accurate to identify infected persons and control transmission. In partnership with APAG Elektronik Corp, a national leader in the manufacturing of electronics and lighting components, we will design and produce a semiconductor-based lab-on-a-chip detection that is based on electrical sensing and detection of viral loads. This non-PCR technique will detect the virus from the electrical signal generated from the binding of COVID-19 virus S protein with human angiotensin converting enzyme-2 (ACE2) protein. The device will be rapidly manufacturable via a combination of cost-effective 3D printing, screen printing and integrated electrical readout. This NSERC Alliance grant will be a first-time collaboration between the research team from the University of Windsor and APAG Elektronik Corp to develop a new method for COVID-19 point of care testing by combining the sensor development skills of the applicants and the electronic manufacturing and distribution skills of the industry partner.

在疫苗研发出来之前，减少 COVID-19 传播的能力直接取决于检测和隔离感染者的能力。目前，COVID-19 检测是在复杂的实验室设置中进行的，需要时间和将样本运送到指定实验室。准确、快速的现场和护理点 (POC) 检测不仅在加拿大而且在全世界范围内对于早期干预和感染控制都至关重要。这种设备的开发将对我们抗击 COVID-19 产生巨大影响。大多数 COVID-19 检测都是基于病毒 RNA 的定量逆转录聚合酶链反应 (qRT-PCR)。 PCR 需要数小时才能得出结果，并且需要实验室设置。便携式等温核酸扩增和环介导等温扩增（LAMP）系统速度快，但由于成本较高，不适合广泛使用。另外，基于抗体的 POC 具有成本效益，但它只能检测个体之前是否已被感染。当前技术中的这些缺陷阻碍了与 COVID-19 等传染性病毒的斗争，测试必须快速（几分钟而不是几小时）、成本效益高、便携且高度准确，以识别感染者并控制传播。我们将与全国领先的电子和照明元件制造商 APAG Elektronik Corp 合作，设计和生产基于半导体的芯片实验室检测，该检测基于电传感和病毒载量检测。这种非 PCR 技术将通过 COVID-19 病毒 S 蛋白与人血管紧张素转换酶 2 (ACE2) 蛋白结合产生的电信号来检测病毒。该设备将通过结合经济高效的 3D 打印、丝网印刷和集成电子读出装置来快速制造。 NSERC 联盟的这项资助将是温莎大学研究团队和 APAG Elektronik Corp 之间的首次合作，通过结合申请人的传感器开发技能和电子制造技术，开发一种用于 COVID-19 护理点测试的新方法以及行业合作伙伴的分销技能。