Development of lab on a chip for rapid and accurate point of care detection of COVID-19

开发芯片实验室，用于快速、准确地检测 COVID-19

• 批准号: 552043-2020
• 负责人: Ahamed, Mohammed
• 金额: $ 3.64万
• 依托单位: University of Windsor
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=703303
• 关键词: Development; lab; chip; rapid; accurate

Until a vaccine is developed, the ability to reduce the transmission of COVID-19 is directly dependent on the capacity to test and isolating the infected. Currently, COVID-19 testing is performed in a sophisticated laboratory setup that requires time and sample routing to designated labs. Accurate, rapid, onsite and point of care (POC) detection has paramount importance not only in Canada but also worldwide for early intervention and infection control. Development of such a device will be highly impactful for our fight against COVID-19. Most of the COVID-19 test are based on quantitative reverse transcription polymerase chain reaction (qRT-PCR) of the virus RNA. PCR takes hours to reach a result and requires laboratory settings. Portable isothermal nucleic acid amplification and loop-mediated isothermal amplification (LAMP) systems are fast but not suitable for wide usage due to the higher cost. Alternately, antibody based POCs offer cost-effective but it can only detect whether an individual has been previously infected or not. These drawbacks in the current technologies are hindering the fight a contagious virus such as COVID-19, tests must be quick (minutes not hours), cost effective, portable and be highly accurate to identify infected persons and control transmission. In partnership with APAG Elektronik Corp, a national leader in the manufacturing of electronics and lighting components, we will design and produce a semiconductor-based lab-on-a-chip detection that is based on electrical sensing and detection of viral loads. This non-PCR technique will detect the virus from the electrical signal generated from the binding of COVID-19 virus S protein with human angiotensin converting enzyme-2 (ACE2) protein. The device will be rapidly manufacturable via a combination of cost-effective 3D printing, screen printing and integrated electrical readout. This NSERC Alliance grant will be a first-time collaboration between the research team from the University of Windsor and APAG Elektronik Corp to develop a new method for COVID-19 point of care testing by combining the sensor development skills of the applicants and the electronic manufacturing and distribution skills of the industry partner.

在开发疫苗之前，减少Covid-19的传播的能力直接取决于测试和隔离感染的能力。当前，在复杂的实验室设置中进行了Covid-19测试，该设置需要时间和样本路由指定实验室。准确，快速，现场和护理点（POC）检测不仅在加拿大，而且在全球范围内对于早期干预和感染控制也至关重要。这种设备的开发将对我们与Covid-19的斗争产生重大影响。大多数COVID-19测试基于病毒RNA的定量逆转录聚合酶链反应（QRT-PCR）。 PCR需要数小时才能达到结果，需要实验室设置。便携式等温核酸扩增和循环介导的等温扩增（LAMP）系统很快，但由于成本较高，因此不适合广泛使用。或者，基于抗体的POC提供具有成本效益的POC，但它只能检测到个人是否已被感染。当前技术中的这些缺点正在阻碍战斗的传染性病毒，例如COVID-19，测试必须很快（分钟而不是小时），具有成本效益，便携式和高度准确，以识别受感染者和控制传播。与电子和照明组件制造的国家领导者Apag Elektronik Corp合作，我们将设计和生产基于半导体的实验室芯片检测，该检测基于病毒载荷的电感和检测。这种非PCR技术将从Covid-19病毒S蛋白与人血管紧张素转化酶-2（ACE2）蛋白的结合产生的电信号中检测到病毒。该设备将通过具有成本效益的3D打印，丝网印刷和集成电气读数的结合来迅速制造。这项NSERC联盟赠款将是温莎大学和Apag Elektronik Corp的研究团队之间的首次合作，通过结合申请人的传感器开发技能与行业合作伙伴的电子制造和分销技能，开发了一种新的COVID CARE测试方法。