Facility for Real Time Imaging of 3D Bioprinted Neural Tissues

3D 生物打印神经组织实时成像设施

• 批准号: RTI-2020-00147
• 负责人: Willerth, Stephanie
• 金额: $ 10.93万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693762
• 关键词: Facility; Real; Time; Imaging; 3D

The use of 3D bioprinting has become an increasing popular strategy for engineering tissues as it can automate this process while enhancing reproducibility of the printed tissues, saving time and money. This process takes on specifications contained in a digital computer aided design (CAD) file and generates a living structure by printing cells encapsulated in specially formulated bioinks. 3D bioprinting serves as a powerful tool for engineering tissues from stem cells in a rapid and reproducible manner compared to traditional techniques. The Willerth lab uses 3D bioprinting to generate neural tissues from human induced pluripotent stem cells combined with their novel neurobioink as a tool for drug screening. In particular, her lab is an international leader in this field as they are one of the few institutions to possess an Aspect Biosystems RX1 bioprinting system that features Lab-on-a-Printer (LOP) technology. This system offers a novel way to automate the process of engineering physiologically relevant neural tissues that have complicated structures and several types of cells. The complex nature of brain tissue requires precise deposition of multiple cell types in defined structures only achievable with Aspect's RX1 bioprinting platform and its unique, patented LOP technology. The Willerth lab was the first group to publish how to engineer living tissues using this unique technology and they continue to pursue ground breaking research in this area. This proposal will enable the acquisition of real time cell imaging system to be used for monitoring the behavior of cells inside of 3D bioprinted tissues. Our previous instrument broke and thus there is a clear and urgent need to replace our live cell imaging system. Acquiring this equipment will enable the Willerth lab to better characterize their tissue cultures by monitoring their properties in real time and to train highly qualified personnel in this cutting edge bioprinting technology. In particular, this system and its associated software enable the characterization of the physical, chemical, and electrical properties of the 3D bioprinted tissues derived from stem cells. Her laboratory provides a high quality training experience as Dr. Willerth has a strong track record of training diverse HQP as she was awarded the 2018 REACH Award for Excellence in Undergraduate Research-inspired Teaching. Additionally, she currently holds two international exchange grants for promoting HQP training in the area of 3D bioprinting based on her unique facility at the University of Victoria. This equipment will enable training of diverse students in cutting edge technology. Her trainees will also have access to additional resources, including the Stem Cell Network, the International Collaboration on Repair Discoveries, and the B.C. Regenerative Medicine Network.

3D生物打印的使用已成为工程组织越来越多的流行策略，因为它可以在增强印刷组织的可重复性的同时自动化此过程，从而节省了时间和金钱。此过程采用数字计算机辅助设计（CAD）文件中包含的规格，并通过打印包裹在特殊配方的生物互联的单元格中生成生活结构。与传统技术相比，3D Bioprinting以快速且可重复的方式可以用作工程组织的强大工具。 Willerth Lab使用3D生物打印来从人类诱导的多能干细胞中产生神经组织，并结合其新型的神经bioink作为药物筛查的工具。特别是，她的实验室是该领域的国际领导者，因为它们是拥有一个具有Lab-An-A-Printer（LOP）技术的Biosystems RX1生物印刷系统的少数机构之一。该系统提供了一种新型的方法，可以使具有复杂结构和几种类型的细胞的工程生理相关的神经组织自动化。脑组织的复杂性质需要在定义的结构中精确沉积多种细胞类型，只有通过Factex的RX1生物印刷平台及其独特的专利LOP技术才能实现。 Willerth实验室是第一个发布如何使用这项独特技术来设计生活组织的小组，他们继续在这一领域进行突破性的研究。该建议将使实时细胞成像系统的获取能够用于监测3D生物打印组织内部细胞的行为。我们以前的仪器破裂了，因此明确而迫切需要更换我们的活细胞成像系统。获取此设备将使Willerth Lab能够通过实时监测其特性，并在这种尖端生物构图技术中培训高素质的人员来更好地描述其组织培养物。特别是，该系统及其相关的软件能够表征来自干细胞的3D生物打印组织的物理，化学和电性能。她的实验室提供了高质量的培训经验，因为Willerth博士拥有培训多样化的HQP的良好记录，因为她因在本科研究启发的教学方面获得了2018年覆盖范围奖。此外，她目前还持有两项国际交流补助金，用于根据维多利亚大学的独特设施在3D Biopinting领域促进HQP培训。该设备将使尖端技术的不同学生培训。她的受训人员还将获得其他资源，包括干细胞网络，国际维修发现合作以及卑诗省再生医学网络。