Exploring new frontiers of oxygen-17 NMR spectroscopy for chemical and biological applications

探索氧 17 NMR 光谱在化学和生物应用中的新领域

• 批准号: RGPIN-2016-05251
• 负责人: Wu, Gang
• 金额: $ 5.46万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=691866
• 关键词: Exploring; new; frontiers; oxygen; 17

Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique for studying molecular structure, dynamics, and chemical bonding. The development of this technique over the past several decades has revolutionized chemical and biological sciences. However, most successful NMR applications to biological systems such as proteins and nucleic acids are primarily based on detection of atomic nuclei with a nuclear spin quantum number of 1/2 such as 1H, 13C, and 15N. On the other hand, the oxygen element, being one of the most abundant in organic and biological molecules, has not yet been readily accessible by NMR spectroscopy. This is principally because the only NMR-active oxygen isotope, 17O, has a nuclear spin quantum number of 5/2, known as a quadrupolar nucleus. Quadrupolar nuclei are notoriously difficult to study by NMR because they often give rise to very broad NMR signals. As a result, 17O is very often considered to be “NMR invisible” in the context of biological macromolecules. Recently, we have made two breakthroughs to solve this challenging problem. First, we have demonstrated the feasibility of obtaining high resolution 17O NMR spectra for biological macromolecules both in the solid state and in aqueous solution. Second, we have shown that 17O NMR is also possible for paramagnetic compounds. The primary objective of our proposed research program for the next 5 years is to apply these new 17O NMR methods to solve important problems in the following three major areas: (1) detection of unstable reaction products, (2) further improvement of resolution at 36 T (the strongest NMR magnet in the world), and (3) studies of paramagnetic metalloproteins. The ultimate goal of our research program is to make all major elements (H, C, N, and O) found in organic and biological molecules equally accessible by NMR spectroscopy. The proposed research program will provide excellent opportunities for training of the next generation of highly qualified personnel to be competitive at the international level. **

核磁性性可爱（NMR）光谱是一种强大的技术，用于研究过去的分子结构的发展，用于阳离子的阳离子，例如蛋白质和核心，是基于原子核的主要基础例如1h，15n的手，是有机和生物分子中最丰富的氧气元素，它不容易被NMR光谱核量子数为5/2，被称为四核核，我们首先做出了两个突破性的问题。顺磁化合物可能是我们提出的临近研究计划的主要目标是应用这些新的17o NMR方法在以下三个主要领域中解决：（2）在36 T时进一步改善分辨率（最强的NMR）世界上的磁铁和（3）磁磁性金属蛋白的研究。 **