Cost effective measurement of root exudates

根系分泌物的经济有效测量

• 批准号: RTI-2020-00244
• 负责人: Lamb, Eric
• 金额: $ 4.45万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=691098
• 关键词: Cost; effective; measurement; root; exudates

The proposed automated solid-phase extraction system will support five basic and applied research programs in the areas of plant ecology, plant-soil interactions, soil remediation, restoration ecology, and soil carbon dynamics. We collaborate on diverse research projects focused on how plants in both natural and agricultural systems interact with and structure the communities of bacteria and fungi in the soil. We are particularly interested in understanding how plant influences on the soil in turn drive the provision of soil ecosystem services such as nutrient cycling and plant productivity.******We require the solid-phase extraction system to accurately and efficiently measure plant root exudates. Plant root soil interactions are governed by plant root exudates, which are low molecular weight organic compounds that are released from plant roots at low (ppb to ppt) concentrations. Changes in plant community composition can alter the concentration and chemical composition of root exudates, causing changes in both soil microbial community structure and the provision of ecosystem services such as nutrient cycling rates and plant productivity. Our research programs depend on the rapid and accurate identification and measurement of root exudate compounds extracted from field collected soil samples. Currently, we use a labour and reagent intensive procedure to extract and prepare root exudates for analysis on an ion chromatograph (IC). This extraction step is a bottleneck in our shared laboratory limiting the number of samples that can be processed. The proposed solid phase extraction system will reduce laboratory costs and time, allowing us to improve sample sizes and reduce laboratory delays for HQP.******Among the many anticipated outcomes of research facilitated by this equipment, we will be able to 1) disentangle the complex multi-way interactions among the invasive plant species, environmental conditions, and the intensity of herbivory, that structure soil communities, 2) link the microbiome, soil, and plant phenotypes associated with stable crop yields across environmental conditions. Identifying the bacterial and fungal species associated with yield stability will allow us to identify the plant physiological and genetic factors linking plant varieties and soil communities, providing critical information for plant breeders to incorporate the microbiome into crop development. 3) We will evaluate how the foundational plant species modify the environmental conditions on a disturbed site, creating conditions that facilitate the establishment of other species. Improving our understanding of early niche construction will improve current models of arctic tundra succession and enable the effective use of native tundra species in restoration.

拟议的自动化固相提取系统将支持植物生态学，植物土壤相互作用，土壤修复，恢复生态学和土壤碳动态领域的五个基本和应用研究计划。我们在不同的研究项目上进行合作，重点介绍了自然和农业系统中的植物如何与土壤中细菌和真菌的群落相互作用并构造。我们特别有兴趣了解植物对土壤的影响反过来又推动了土壤生态系统服务（例如养分循环和植物生产力）的提供。植物根部土壤相互作用受植物根根渗出液的控制，它们是低分子量有机化合物，这些化合物从低（PPB至PPT）浓度的植物根中释放出来。植物群落组成的变化会改变根部渗出液的浓度和化学成分，从而导致土壤微生物群落结构的变化以及提供生态系统服务（例如营养循环速率和植物生产力）的变化。我们的研究计划取决于从现场收集的土壤样品中提取的根渗出液的快速准确鉴定和测量。当前，我们使用劳动力和试剂密集型程序来提取和准备根渗出液，以分析离子色谱仪（IC）。此提取步骤是我们共享的实验室中的瓶颈，限制了可以处理的样品数量。 The proposed solid phase extraction system will reduce laboratory costs and time, allowing us to improve sample sizes and reduce laboratory delays for HQP.******Among the many anticipated outcomes of research facilitated by this equipment, we will be able to 1) disentangle the complex multi-way interactions among the invasive plant species, environmental conditions, and the intensity of herbivory, that structure soil communities, 2) link the microbiome, soil, and plant与稳定的农作物产量相关的表型跨环境条件。确定与产量稳定性相关的细菌和真菌物种将使我们能够确定将植物生理和遗传因素与植物品种和土壤群落联系起来的植物生理和遗传因素，从而为植物育种者提供关键信息，以将微生物组纳入农作物的发育。 3）我们将评估基础植物物种如何修改受干扰地点的环境条件，从而创造有助于建立其他物种的条件。提高我们对早期利基结构的理解将改善当前北极苔原继承模型，并有效利用本地苔原物种在恢复中。