Spirochete coordination of adhesion and motility in the presence of fluid shear forces

存在流体剪切力时螺旋体粘附和运动的协调

基本信息

批准号：
401938-2011
负责人：
Moriarty, Tara
金额：
$ 2.55万
依托单位：
University of Toronto
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2014
资助国家：
加拿大
起止时间：
2014-01-01 至 2015-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=550717
关键词：
Spirochete coordination adhesion motility presence

项目摘要

Spirochetes are highly motile helical or planar wave-form bacteria whose motility is thought to depend on adhesion to components of the extracellular environment. However, if motility is dependent on adhesion, then adhesion complexes must be dynamic for spirochete movement to occur. Spirochetes must also coordinate motility and adhesion in the presence of fluid shear forces, which can disrupt bacterial adhesion and migration on surfaces in the natural environment, and could alter both the motile and adhesive properties of these bacteria. The properties and mechanisms underlying dynamic coordination of spirochete adhesion and motility in the presence of fluid shear forces are not yet defined. Early efforts to investigate the relationship between spirochete motility and adhesion were impeded by the inability to disrupt these functions genetically and the absence of appropriate dynamic live cell observation and labelling methods. Recent advances finally permit investigation of the dynamic coordination of motility and adhesion in a living spirochete, the Lyme disease pathogen Borrelia burgdorferi (Bb). The overall objective of my research program is to investigate the fundamental properties and mechanisms underlying coordination of adhesion and motility in Bb subjected to fluid shear forces. My short-term objective is to use live cell imaging approaches to define the dynamic relationship between spirochete motility and adhesion, and the effect of fluid shear forces on these functions. My long-term objective is to develop appropriate methodologies for investigating the biophysical properties and mechanisms coordinating Bb adhesion and motility under fluid shear forces. These studies will provide the first dynamic insight into mechanisms coordinating motility and adhesion in spirochetes, and the effect of fluid shear forces on these processes.

螺旋体是高度活动的螺旋或平面波形细菌，其活动被认为取决于对细胞外环境成分的粘附。然而，如果运动依赖于粘附，则粘附复合物必须是动态的才能发生螺旋体运动。螺旋体还必须在存在流体剪切力的情况下协调运动和粘附，这会破坏自然环境中表面上的细菌粘附和迁移，并可能改变这些细菌的运动和粘附特性。在存在流体剪切力的情况下，螺旋体粘附和运动的动态协调的特性和机制尚未确定。由于无法从遗传上破坏这些功能以及缺乏适当的动态活细胞观察和标记方法，早期研究螺旋体运动性和粘附之间关系的努力受到阻碍。最近的进展最终允许对活体螺旋体——莱姆病病原体伯氏疏螺旋体（Bb）——运动性和粘附性的动态协调进行研究。我的研究计划的总体目标是研究 Bb 在流体剪切力作用下粘附和运动协调的基本特性和机制。我的短期目标是使用活细胞成像方法来定义螺旋体运动和粘附之间的动态关系，以及流体剪切力对这些功能的影响。我的长期目标是开发适当的方法来研究生物物理特性和协调流体剪切力下 Bb 粘附和运动的机制。这些研究将为螺旋体中协调运动和粘附的机制以及流体剪切力对这些过程的影响提供第一个动态见解。