Dynamics and folding of a membrane protein and transcription activator

膜蛋白和转录激活剂的动力学和折叠

• 批准号: 41618-2011
• 负责人: ONeil, Joe
• 金额: $ 2.91万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=521548
• 关键词: Dynamics; folding; membrane; protein; transcription; Dynamics; folding; membrane; protein; transcription

A lipid membrane surrounds all animal, plant, and bacterial cells. Protein channels and receptors embedded in the lipid precisely control the flow of nutrients, waste products, energy, and information into and out of cells, but are poorly understood. Our long-term goal is to develop methods to overcome the many difficulties in membrane protein structure, folding and dynamics analysis. Recently, we have made progress in studying the folding of a bacterial membrane protein, the glycerol facilitator in detergent environments. We propose to continue this work by applying a new method for synthesizing the protein using cellular extracts and studying folding of the protein in a lipid membrane environment. Cell-free synthesis opens up the possibility of labelling the protein with isotopes for analysis by Nuclear Magnetic Resonance (NMR) spectroscopy, a method analogous to Magnetic Resonance Imaging (MRI). With labeled protein, we will study the detailed atomic dynamics of the protein that are key to understanding its biological transport mechanism. We will also continue our work along similar lines on the fungal antibiotic peptide alamethicin that is a model membrane protein.

脂质膜围绕着所有动物，植物和细菌细胞。嵌入脂质中的蛋白质通道和受体精确地控制了养分，废物产物，能量和信息的流动，但知识却很少。我们的长期目标是开发克服膜蛋白结构，折叠和动态分析的许多困难的方法。最近，我们在研究细菌膜蛋白的折叠方面取得了进展，这是洗涤剂环境中的甘油促进剂。我们建议通过应用一种使用细胞提取物合成蛋白质并研究脂质膜环境中蛋白质折叠的新方法来继续这项工作。 无细胞的合成为通过核磁共振（NMR）光谱法分析蛋白质标记蛋白质的可能性是一种类似于磁共振成像（MRI）的方法。 使用标记的蛋白质，我们将研究蛋白质的详细原子动力学，这是了解其生物运输机制的关键。我们还将继续在与模型膜蛋白的真菌抗生素肽alamethicin上继续进行类似的工作。