Biological, functional and radiographic evaluation of autologous chondrocyte implantation

自体软骨细胞植入的生物学、功能和放射学评估

• 批准号: nhmrc : 254743
• 负责人: Prof David Wood
• 金额: $ 20.5万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Development Grants
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/biological-functional-radiographic-chondrocyte-implantation/97059
• 关键词: Biological; functional; radiographic; evaluation; autologous

We will test the hypothesis that autologous chondrocyte implantation (ACI) and extensor realignment produces superior clinical, biological and radiographic results when compared with conventional treatment of realignment and debridement. We will specifically address the following aims: 1. Quantify the clinical outcome of ACI compared to the traditionally used treatment of debridement through the use of functional evaluation in a blinded randomised controlled clinical trial; 2. Evaluate the radiographic changes in the patellofemoral joint that occur as a result of ACI, using high resolution magnetic resonance imaging (MRI) to quantify the regeneration of hyaline articular cartilage; 3. Using the new technique of confocal arthroscopy, we will compare the histologic appearances of the ACI graft and its interface with adjacent articular cartilage; 4. Evaluate patient, surgical and explant chondrocyte characteristics in relation to functional, radiographic and biological outcomes. With respect to the matrix-induced autologous chondrocyte implantation (MACI) technique, we wish to clarify the clinical practice as a definitive treatment for articular cartilage defects. This will be the first randomised, controlled clinical trial of the MACI technique compared to that used by other groups. A positive clinical outcome from this trial will help promote the three cornerstones of this procedure – successful cell culture, efficient surgical procedures, and complimentary postoperative rehabilitation. Furthermore, this research will: a) Enhance the expansion of the MACI technique; b) Encourage development of endoscopic techniques of implantation using a combination of – • Preoperative defect registration with MRI • pre-cut custom patches, implanted with • Computer-assisted navigation techniques: c) Increase the potential to cater for a larger number of patients requiring articular cartilage repair; d) Confirm the long-term durability of regenerated cartilage in the 4th year and beyond; e) Add further commercial value by demonstrating MACI may prevent the onset of osteoarthritis.

我们将检验以下假设：与常规的重新调整和调试相比，自体软骨细胞（ACI）和扩展器重新调整可产生优越的临床，生物学和射线照相结果。我们将具体解决以下目的：1。与传统使用的通过功能评估在一项盲目的随机对照临床试验中使用功能评估的处理方法相比，量化ACI的临床结果； 2。使用高分辨率磁共振成像（MRI）来量化透明关节镜检查的再生，评估ACI导致的patelo股关节的射线照相变化； 3。使用共聚焦关节镜的新技术，我们将比较ACI移植物的组织学外观及其界面与相邻的围栏软骨。 4。评估与功能，放射学和生物学结局有关的患者，外科和外科软骨细胞特征。关于基质诱导的自体软骨细胞植入（MACI）技术，我们希望阐明临床实践，以作为关节软骨缺陷的明确治疗方法。与其他小组相比，这将是MACI技术的首次随机，对照临床试验。这项试验的积极临床结果将有助于促进该程序的三个基石 - 成功的细胞培养，有效的手术程序和术后免费的康复。此外，这项研究将：a）增强MACI技术的扩展； b）鼓励使用 - •术前缺陷注册与MRI的组合开发植入的内镜技术•植入•植入•计算机辅助导航技术的定制贴片：c）提高了需要关注关节软骨修复的更多患者的潜力； d）确认第四年及以后再生软骨的长期耐用性； e）通过证明MACI可以防止骨关节炎的发作来增加进一步的商业价值。