Properties of photoreceptors and Muller cells investigated with AO-OCT

使用 AO-OCT 研究光感受器和 Muller 细胞的特性

• 批准号: 9012652
• 负责人: Ravi S. Jonnal
• 金额: $ 9.78万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9012652
• 关键词: Affect; Anatomy; Award; Cell physiology; Cells; Cellular Morphology; Cellular Structures; Classification; Clinical; Computer Analysis; Computing Methodologies; Cone; Custom; Data; Dependence; Development; Diagnosis; Dimensions; Disease; Electroretinography; Exhibits; Eye diseases; Fluorescein; Grant; Human; Image; Individual; Individual Differences; Kinetics; Knowledge; Laboratories; Lateral; Lead; Learning; Life; Light; Link; Measures; Membrane; Mentors; Methods; Microscopic; Modification; Morphology; Muller' s cell; Ophthalmology; Optical Coherence Tomography; Optical Methods; Optics; Phase; Photoreceptors; Play; Predisposition; Property; Psychophysics; Recovery; Research; Resolution; Retina; Retinal; Retinal Diseases; Role; Scanning; Scientist; Source; Staging; Stimulus; Structure; System; Techniques; Testing; Three-Dimensional Imaging; Time; Training; Uncertainty; Vision; Work; Writing; adaptive optics; base; career; experience; eye center; imaging system; instrumentation; interest; novel; optical fiber; public health relevance; response; retinal rods; three dimensional structure; tool; vision science

 DESCRIPTION (provided by applicant): The research proposed for this award will examine functional and structural properties of single cells in the human retina. The three Aims will link the candidate's scientific background and career plans to study the human retina using a combination of adaptive optics (AO) and optical coherence tomography (OCT) which provide high lateral and high axial resolution, respectively (~2.5 μm in all three dimensions). Aim 1 will use AO-OCT to determine how the microscopic structure of the photoreceptors gives rise to the bands of the outer retina visible in routine clinical images-bands whose anatomical origins are not known precisely. It will test the hypothesis about whether the third band is from multiple reflectors in the interdigitation zone of the RPE or the tips of individual photoreceptors. Aim 1 will also test hypotheses about the contributions of rods and S-cones to the OCT image. Aim 2 will exploit this knowledge to noninvasively measure function in single photoreceptors, and identify a subset of cones, putative S-cones, often thought to be the first cone type to be affected by outer retinal disease. Aim 3 will study the optical properties of the Müller cells whih are known to sustain retinal cells, and have been proposed, controversially, to also act as optical fibers for guiding light to the photoreceptors. This Aim will test ideas about Müller cell wave-guiding properties, for the first time, in the living retina. The optical signatures of all ofthe retinal layers studied in each of the three Aims are seen in clinical OCT images, but their cellula origins are uncertain. The proposed research will thus provide data fundamental to understanding retinal structure and function, and will enrich our understanding of clinical OCT images of retinal disease. During the mentored phase of this Award, the candidate will draw on all phases of his previous vision science experience including adaptive optics and computational analysis of the 3D structure of retinal images, and will expand this experience in significant ways needed for his career plans. This work will be conducted in the laboratory of John S. Werner where AO-OCT instrumentation has been pioneered and conveniently located in the UC Davis Eye Center. One AO-OCT system is available for the candidate's modification and exclusive use. Training will be buttressed by mentoring and didactic courses with advisors, Edward N. Pugh, Jr. and Lawrence S. Morse, who are internationally renowned basic and clinical vision scientists, respectively. Additional didactic components include a 'Fluorescein' course normally taken by residents and fellows in Ophthalmology and a grant-writing course. The training provided through this K99 will set the stage for the candidate's independent career in vision science research.

 描述（由申请人提供）：该奖项提出的研究将检查人类视网膜中单细胞的功能和结构特性，这三个目标将连接候选人的科学背景和职业计划，以结合自适应光学来研究人类视网膜。 (AO) 和光学相干断层扫描 (OCT) 分别提供高横向和高轴向分辨率（Aim 1 在所有三个维度上约为 2.5 μm）。 使用 AO-OCT 来确定光感受器的微观结构如何产生常规临床图像中可见的外视网膜带（其解剖学起源尚不清楚），这将检验第三条带是否来自多个带的假设。目标 1 还将测试 RPE 交指区的反射器或单个光感受器尖端的视杆细胞和 S 锥体对 OCT 图像的贡献的假设。无创地测量单个光感受器的功能，并识别视锥细胞的子集，即假定的 S 视锥细胞，通常被认为是第一个受外视网膜疾病影响的视锥细胞类型，目标 3 将研究已知的 Müller 细胞的光学特性。维持视网膜细胞，并且有争议地被提议也可以充当将光引导到感光器的光纤。该目标将测试有关穆勒细胞的想法。 这三个目标中研究的所有视网膜层的光学特征首次在临床 OCT 图像中看到，但其细胞起源尚不确定。提供了解视网膜结构和功能的基础数据，并将丰富我们对视网膜疾病临床 OCT 图像的理解。在该奖项的指导阶段，候选人将利用其先前视觉科学经验的所有阶段，包括自适应光学和计算分析。的视网膜图像的 3D 结构，并将以显着的方式扩展这种体验 这项工作将在 John S. Werner 的实验室中进行，该实验室率先使用了 AO-OCT 仪器，并且位于加州大学戴维斯分校眼科中心，方便候选人进行修改和专有。培训将通过顾问 Edward N. Pugh, Jr. 和 Lawrence S. Morse 的指导和教学课程得到支持，他们分别是国际知名的基础和临床视觉科学家。 “荧光素”课程通常由眼科住院医师和研究员参加，并且通过 K99 提供的培训将为候选人在视觉科学研究方面的独立职业生涯奠定基础。