Optical measurement of cellular oxygenation in shock and resuscitation

休克和复苏时细胞氧合的光学测量

• 批准号: 7893505
• 负责人: KENNETH A SCHENKMAN
• 金额: $ 23.4万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7893505
• 关键词: Accident and Emergency department; Accounting; Acids; Ambulances; Animal Model; Blood; Blood Pressure; Brain; Caring; Cell Hypoxia; Cells; Central venous pressure; Cerebrum; Cessation of life; Clinical; Color; Critical Care; Critical Illness; Data; Devices; Goals; Heart; Hemoglobin; Hemorrhage; Hemorrhagic Shock; Injury; Intensive Care Units; Kidney; Knowledge; Laboratories; Least-Squares Analysis; Light; Magnetic Resonance Spectroscopy; Measurement; Measures; Medical; Methods; Modeling; Monitor; Multiple Organ Failure; Muscle Cells; Myoglobin; Optics; Organ; Oryctolagus cuniculus; Oxygen; Patients; Pulse Oximetry; Research; Resolution; Resuscitation; Role; Severities; Shock; Signal Transduction; Skeletal Muscle; Skin; Spectrum Analysis; Testing; Time; Tissues; Trauma; Traumatic Shock; base; improved; in vivo; indexing; innovation; instrument; light scattering; monitoring device; novel; novel strategies; pressure; public health relevance; success

DESCRIPTION (provided by applicant): Critically ill patients may die of Multiple Organ Dysfunction Syndrome (MODS) despite having their blood pressure, arterial oxygenation, and blood acid base status restored. Indirect evidence indicates that cellular hypoxia is the initial injury leading to death in these patients. This is a revised proposal to develop a noninvasive oximeter to measure skeletal muscle cellular oxygenation. Pulse oximetry revolutionized critical care by providing a noninvasive measurement of arterial hemoglobin oxygen saturation. The innovative goal of the present application is to go the next step and develop an instrument for noninvasively measuring skeletal muscle cellular oxygenation by determining intracellular myoglobin oxygen saturation. Preliminary data are presented that demonstrate the feasibility of the method. A research plan is proposed where a novel optical spectral analysis method, Multivariate Curve Resolution (MCR), is optimized to account for light scattering in tissue. The method will be validated by an independent measure of myoglobin saturation using Magnetic Resonance Spectroscopy (MRS). The utility of the instrument will be tested in a model of hemorrhagic shock in anesthetized rabbits. The ability to noninvasively monitor skeletal muscle cellular hypoxia has enormous potential for the treatment of critically ill patients. PUBLIC HEALTH RELEVANCE: This project will develop and test a new approach to assess cellular oxygenation in skeletal muscle. The approach analyzes spectral, or color changes from reflected light through the skin to determine how well oxygen is able to get into muscle cells, in an animal model of traumatic shock. This new approach may provide crucial new information that can be used for improving current therapies for people in shock, resulting from traumatic injuries.

描述（申请人提供）：危重患者尽管血压、动脉氧合和血液酸碱状态已恢复，但仍可能死于多器官功能障碍综合征（MODS）。间接证据表明细胞缺氧是导致这些患者死亡的最初损伤。这是一项修订提案，旨在开发一种无创血氧计来测量骨骼肌细胞氧合。脉搏血氧仪通过提供动脉血红蛋白氧饱和度的无创测量彻底改变了重症监护。本申请的创新目标是下一步开发一种通过确定细胞内肌红蛋白氧饱和度来无创测量骨骼肌细胞氧合的仪器。初步数据证明了该方法的可行性。提出了一项研究计划，其中优化了一种新型光谱分析方法——多元曲线分辨率（MCR），以解释组织中的光散射。该方法将通过使用磁共振波谱 (MRS) 独立测量肌红蛋白饱和度进行验证。该仪器的实用性将在麻醉兔子的失血性休克模型中进行测试。无创监测骨骼肌细胞缺氧的能力对于治疗危重患者具有巨大的潜力。 公共健康相关性：该项目将开发和测试一种评估骨骼肌细胞氧合的新方法。该方法分析穿过皮肤的反射光的光谱或颜色变化，以确定在创伤性休克的动物模型中氧气进入肌肉细胞的能力。这种新方法可能提供重要的新信息，可用于改善目前对因外伤而休克的人的治疗。