Light-propelled dental adhesives with enhanced bonding capability

具有增强粘合能力的光驱动牙科粘合剂

• 批准号: 10741660
• 负责人: Devatha P. Nair
• 金额: $ 42.9万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-12 至 2025-07-11
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10741660
• 关键词: Address; Adhesions; Adhesives; Amalgam; Artificial Saliva; Bisphenol A-Glycidyl Methacrylate; Collagen; Collagen Fibril; Color; Complex; Composite Resins; Dental; Dentin; Diffusion; Ensure; Environment; Enzymes; Esthetics; Ethanol; Fatigue; Formulation; Goals; Hybrids; Hydrophobic Interactions; Hydrophobicity; Hydroxyapatites; Infiltration; Kinetics; Light; Link; Longevity; Matrix Metalloproteinases; Mechanics; Methacrylates; Movement; NanoGel; Nanostructures; Oral; Oral cavity; Penetration; Performance; Plant Resins; Play; Polymers; Predisposition; Protocols documentation; Research; Residual state; Role; Sampling; Scheme; Source; Surface; Swelling; Techniques; Tooth structure; Visible Radiation; Water; Wettability; Work; amphiphilicity; azobenzene; bisphenol A; collagen scaffold; collagenase; composite restoration; conditioning; copolymer; crosslink; demineralization; dental adhesive; design; functional group; hydrophilicity; interfacial; mechanical properties; monomer; nanopolymer; novel; pi bond; polymerization; preference; preservation; pressure; prevent; restoration; restorative dentistry; restorative material; tooth surface

Project Summary/Abstract: The growing preference for aesthetically pleasing composite restorations is reflected in the over 122 million dental restorations placed annually in the U.S. However, the replacement of failed restorations accounts for greater than 50% of all restorative dental work. The relatively short duration composite restorations last in comparison to traditional restorations (e.g., amalgam) is attributed to a weak hybrid layer, which is the layer that bonds the adhesive to the tooth structure. Bonding to dentin in the tooth in a consistent and stable manner is challenging. While applying the adhesive resin on etched dentin, relying on simple diffusion to homogenously infiltrate the dentinal tubules up to the etch depth in the presence of pulpal pressure makes adhering to dentin less predictable and more technique sensitive. Leaving exposed collagen within the hybrid layer compromises its mechanical strength and long-term stability by leaving it vulnerable to proteolytic degradation. We propose a solution to this problem by introducing light-responsive, methacrylated nanoadditives that swell and disperse within conventional adhesives and are designed to move away from a visible light source. By using light-induced resin mobility, we propose to drive the controlled, light-activated, homogenous diffusion of hydrophobic and hydrophilic adhesive resin components within dentinal tubules up to the depth of etching. This will ensure that the demineralized surfaces and exposed collagen networks are now evenly and homogenously enveloped by resin within the hybrid layer to form a mechanically robust anchor with the tooth. Subsequently, pendant functionality on the surface of the nanoadditives will further enhance the strength of the resin-dentin interface and the stability of the collagen by introducing crosslinking via primary and secondary bonds. The presence of labile functional groups that can induce intermolecular and intermicrofibrillar collagen crosslinks, along with hydrophobic interactions can enhance the mechanical properties and biostability of the adhesive layer. Increased crosslinking will also decrease the susceptibility of the hybrid layer to bacterial collagenase and MMPs. Therefore, we will specifically aim to 1) synthesize 4 to 6 light-responsive nanoadditives that can be incorporated within conventional adhesive resins and study adhesive resin propulsion as a function of 430-480 nm light exposure, polymerization kinetics, degree of conversion, and mechanical properties under simulated pulpal pressure. Toward this end, light-responsive nanoadditives will be synthesized using a solution polymerization protocol and the adhesive resin-nanoadditive networks will be characterized based on the type and concentration of light-responsive moieties within the networks. Pendant functionality introduced via nanoadditives to enhance bioadhesion and collagen crosslinking will also be evaluated. In Aim 2, we will establish the conditions by which the nanoadditives can enhance bioadhesion within the hybrid layer via additional crosslinking and characterize the µTBS, interfacial gap formation, and stability of the resin-tooth interface in the oral physiochemical environment.

项目摘要/摘要： 超过 1.22 亿个牙科修复体反映了人们对美观的复合修复体日益增长的偏好 美国每年都会进行修复体修复，但更换失败修复体的比例超过 50% 与传统修复体相比，修复复合材料的持续时间相对较短。 （例如汞合金）归因于弱混合层，该层是将粘合剂粘合到牙齿结构上的层。 在蚀刻牙本质上应用粘合树脂时，以一致且稳定的方式粘合牙齿中的牙本质是具有挑战性的。 在牙髓存在的情况下，依靠简单的扩散均匀地渗透牙本质小管直至蚀刻深度 压力使得牙本质的粘附更难以预测，并且在混合体中留下暴露的胶原蛋白。 层容易发生蛋白水解降解，从而损害其机械强度和长期稳定性。 通过引入光响应、可溶胀和分散的甲基丙烯酸酯纳米添加剂提出了解决该问题的方案 在传统粘合剂内，并设计为通过使用光诱导树脂远离可见光源。 流动性，我们建议驱动疏水性和亲水性的受控、光激活、均匀扩散 牙本质小管内的粘合树脂成分达到蚀刻深度，这将确保脱矿质。 表面和暴露的胶原蛋白网络现在被混合层内的树脂均匀地包裹着 与牙齿形成机械坚固的锚定，随后在纳米添加剂的表面上形成悬垂功能。 通过引入交联进一步增强树脂-牙本质界面的强度和胶原蛋白的稳定性 通过主要和次要键的存在，可以诱导分子间和 微原纤维间的胶原交联以及疏水相互作用可以增强机械性能和 粘合层的生物稳定性增加也会降低杂化层对细菌的敏感性。 因此，我们将特别致力于 1）合成 4 至 6 种光响应纳米添加剂。 可合并到传统的粘合树脂中，并研究粘合树脂推进力与 430-480 nm 光的函数关系 模拟牙髓压力下的暴露、聚合动力学、转化度和机械性能。 为此，将使用溶液聚合方案和光响应纳米添加剂来合成 粘合树脂纳米添加剂网络将根据光响应部分的类型和浓度进行表征 通过纳米添加剂引入悬垂功能，以增强生物粘附和胶原交联。 在目标 2 中，我们将建立纳米添加剂增强生物粘附的条件。 通过额外的交联在混合层内进行表征，并表征 µTBS、界面间隙的形成和稳定性 口腔理化环境中的树脂-牙齿界面。