Core A: Ultrastructural Assessment & Human Tissue

核心 A：超微结构评估

• 批准号: 10627575
• 负责人: Olujimi A Ajijola
• 金额: $ 33.97万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-10 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627575
• 关键词: 3-Dimensional; Achievement; Adrenergic Agents; Biological; Cardiac; Cardiomyopathies; Chemicals; Cicatrix; Data Set; Ensure; Family suidae; Functional disorder; Goals; Heart; Heart Diseases; Heart Transplantation; Human; Human Resources; Image; Infrastructure; Ion Channel; Lasers; Link; Lung; Maps; Methods; Molecular; Molecular Biology; Myocardial; Organ Procurements; Organ Transplantation; Patients; Penetration; Process; Productivity; Protocols documentation; Pulmonary Heart Disease; Recovery; Research Personnel; Resolution; Services; Structure; Structure of stellate ganglion; Sudden Death; Techniques; Testing; Therapeutic; Time; Tissue Procurements; Tissues; Validation; cell type; cost; experience; high resolution imaging; human disease; human tissue; innovation; instrumentation; member; mortality risk; neural; neuroregulation; neurotransmission; success; transcriptomics

SUMMARY/ABSTRACT – Scientific Core - Structure The goal of the Ultrastructural Assessment and Human Tissue Core is to maximize the scientific progress and productivity of the PPG investigators by providing essential services. The primary function of Core A is to serve human and porcine myocardial and stellate ganglion tissue needs across all projects and Core B. Given significant regulatory and logistical hurdles needed to procure and study human heart and stellate ganglion tissues, it is ideal for this to be handled by a central core. The core will perform three key functions for the study: 1) tissue clearing and ultrastructural imaging; 2) procurement of normal (or near normal) and diseased human tissues, specifically hearts and stellate ganglia; and 3) molecular biology. All 3 projects of this PPG with utilize at least two of these services, and there will be tight links between this core and Core B, the Functional Assessment and Instrumentation Core. Over the past 15 months, we have built a pipeline to obtain diseased hearts from patients undergoing orthotopic heart transplantation, and normal or near normal hearts rejected for organ transplant. Our infrastructure for obtained stellate ganglia from patients with cardiomyopathy undergoing stellectomy, and stellate ganglia from patients without cardiopulmonary disease at the time of heart and/or lung donation has existed for 5 years. Given the unpredictable times at which these tissues become available, centralizing the tissue procurement activities within this core will benefit the PPG by streamlining protocols and approvals, minimizing cost, and boosting productivity. The Core will also streamline the 4-week process of tissue clearing from pigs and humans by batching tissues to minimize error, tissue clearing involves numerous steps and chemicals. Finally, it is most ideal that tissues are cleared and imaged in high resolution in close coordination with study team members from Projects 1-3 in this Core whether or not the tissues are cleared. For the duration of the PPG, the core is poised – with experience, robust protocols, and experienced personnel – to provide critical services that ensure the success of this PPG.

摘要/摘要 – 科学核心 – 结构 超微结构评估和人体组织核心的目标是最大限度地促进科学进步和 通过提供基本服务提高 PPG 调查人员的工作效率 核心 A 的主要职能是提供服务。 所有项目和核心 B 中人类和猪心肌和星状神经节组织的需求。 获取和研究人类心脏和星状神经节需要重大的监管和后勤障碍 组织，这是由中央核心处理的理想选择，该核心将为组织执行三个关键功能。 研究：1）组织透明化和超微结构成像；2）获取正常（或接近正常）和患病的组织 人体组织，特别是心脏和星状神经节；3) 该 PPG 的所有 3 个项目。 至少利用其中两项服务，并且该核心与核心 B（功能性）之间将存在紧密联系 评估和仪器核心 在过去 15 个月里，我们建立了一条获取疾病的管道。 来自接受原位心脏移植的患者的心脏，以及正常或接近正常的心脏被拒绝 我们从患有心肌病的患者身上获得星状神经节的基础设施。 心脏和/或肺时没有心肺疾病的患者的星状切除术和星状神经节 鉴于这些组织的可用时间不可预测，捐赠已经存在了 5 年。 将组织采购活动集中在此核心内将通过简化协议和 The Core 还将简化为期 4 周的流程。 通过分批组织来清除猪和人类的组织以最大程度地减少错误，组织清除涉及大量 最后，最理想的情况是组织被清除并近距离以高分辨率成像。 与本核心项目 1-3 的研究团队成员进行协调，无论组织是否被清除。 在 PPG 期间，核心人员已做好准备 – 拥有丰富的经验、健全的协议和经验丰富的人员 – 提供关键服务以确保本次 PPG 的成功。