Cell and Molecular Analysis Core - "Mentoring in Cardiovascular Biology

细胞和分子分析核心-“心血管生物学的指导”

• 批准号: 9126993
• 负责人: Catalin Filipeanu
• 金额: $ 13.38万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9126993
• 关键词: Achievement; Animals; Biology; Cardiovascular Diseases; Cardiovascular system; Cause of Death; Cell Culture Techniques; Cells; Cellular biology; Centers of Research Excellence; Complex; Data; Data Analyses; Electron Spin Resonance Spectroscopy; Environment; Equipment; Flow Cytometry; Foundations; Generations; High Pressure Liquid Chromatography; Human; Human Resources; Image; Institution; Investments; Laboratories; Louisiana; Mass Spectrum Analysis; Mentors; Methodology; Methods; Modeling; Molecular; Molecular Analysis; Molecular Biology; Morbidity - disease rate; National Center for Research Resources; Phase; Procedures; Proteins; Proteomics; Research; Research Personnel; Resources; Services; System; Training and Education; Translational Research; United States; Universities; base; instrumentation; mortality; programs; success

Today, the advance in translational research requires complex integration of molecular, cellular, animal and human models. This multitude of approaches exceeds the possibilities of a 'standard' laboratory and demands a specialized environment to enhance the success rate and increase the competiveness of our investigators. The Cell and Molecular Analysis Core was developed starting in the second year ofthe Phase I COBRE program. Today this core offers our researchers access to a unique facility on the LSUHSC campus, which provides the latest state-of the-art methodological approaches in cell and molecular biology. This core provides skilled personnel to assist with and/or perform highly specialized experimental procedures. This core is coordinated by Drs. Catalin Filipeanu and Arthur Haas and is organized in the following units: Proteomics, Mass Spectrometry, Electron Paramagnetic Resonance (EPR) Imaging, HPLC, Cell Culture, and Flow-cytometry. The Specific Aims of this core are: 1) to provide skilled personnel to assist with and/or perform highly specialized procedures for HPLC separation of proteins, 2D separation of proteins for proteomic analysis by mass spectrometry, cell culture, and EPR imaging, 2) to maintain the resources for safe and effective use 3) to provide programs of education and training ofthe methods and instrumentation available in the Core, and 4) to assist investigators in the generation of project data. These facilities are open to all COBRE investigators, as well as researchers throughout the LSUHSC and other institutions. The availability of multiple types of advanced equipment, sophisticated data analysis systems, and specialized personnel dramatically increase the research achievements by current and previous COBRE and LSUHSC investigators. The COBRE Phase III transition for this Core builds upon the previous investments that our University committed to the NCRR Phase I and Phase II programs. In Phase II, the services provided by this COBRE Cell and Molecular Analysis core were integrated under the umbrella ofthe LSUHSC Proteomics Facility, thus providing the foundation for transformation of this successful core to an Institutional facility.

如今，转化研究的进步需要分子，细胞，动物和 人类模型。这种多种方法超出了“标准”实验室的可能性和 需要一个专业的环境来提高成功率并提高我们的竞争力 调查人员。细胞和分子分析核心是从该相的第二年开始的 我的鞋垫计划。今天，这个核心使我们的研究人员可以访问LSUHSC上的独特设施 校园，提供了细胞和分子生物学中最新的方法论方法。 该核心提供熟练的人员来协助和/或执行高度专业化的实验 程序。该核心由Drs协调。 Catalin Filipeanu和Arthur Haas，在 以下单元：蛋白质组学，质谱法，电子顺磁共振（EPR）成像，HPLC， 细胞培养和流通状态仪。该核心的具体目的是：1）提供熟练的人员来协助 使用和/或执行高度专业的HPLC分离蛋白质，蛋白质的2D分离 用于质谱，细胞培养和EPR成像的蛋白质组学分析，2）维护资源 安全有效的用途3）提供教育计划和培训方法和仪器 在核心中可用，4）可以帮助调查人员生成项目数据。这些设施开放 对于所有毛绒研究人员以及整个LSUHSC和其他机构的研究人员。这 可用性多种类型的高级设备，复杂的数据分析系统和专业 人员通过当前和以前的毛刺和LSUHSC急剧提高研究成就 调查人员。该核心的COBRE第三阶段过渡基于我们先前的投资 大学致力于NCRR第一阶段和第二阶段计划。在第二阶段，提供的服务 在LSUHSC蛋白质组学的伞下整合了毛细胞和分子分析核心 设施，从而为将这种成功的核心转化为机构设施提供了基础。