Flow Cytometry & Cell Sorter Core

流式细胞术

• 批准号: 9095370
• 负责人: LingLin Xie
• 金额: $ 8.69万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9095370
• 关键词: Area; Cell Culture Techniques; Cell Separation; Cells; Core Facility; Development; Distant; Educational process of instructing; Environment; Fellowship; Flow Cytometry; Funding; Health; Health Professional; Home environment; Laboratories; Learning; Medical Students; North Dakota; Population; Process; Research Infrastructure; Research Personnel; Residencies; Services; Sorting - Cell Movement; Stem Cell Research; Technology; base; career; experience; graduate student; research and development; undergraduate student

The development of the core facilities has been a learning experience for the PI of the NO INBRE. The major lesson learned since initial funding of the NO INBRE is that in a low population, large land mass state with a limited investigator base, one must develop core facilities that provide services that cannot be purchased through commercial entities. This certainly applies to flow and cell sorting since there is no other facility capable of sorting cells in the entire state of North Dakota. Likewise, it is very difficult, if not impossible, to perform flow through commercial entities or at academic centers distant from one's home laboratory. This is especially true for the sorting of cells, where in most instances immediate processing and/or culture of the product is necessary. A prime example of this situation is in the cutting-edge technology of stem cells, where research in this area is difficult, if not impossible, without the ability to sort and capture the resulting cells for culture and/or analysis. The NO INBRE has identified this core as essential for the further development of research, teaching and service in NO. Simply stated, at some juncture in their undergraduate career, every undergraduate student destined to enter the graduate student or health professional pipeline should have been exposed to flow and cell sorting. The same is true for graduate students destined for postdoctoral fellowships and medical students progressing to residencies.

核心设施的发展一直是No Inbre的PI的学习经验。自No Inbre最初资助以来，学到的主要经验是，在人口较低的人口较低的土地群体状态下，调查员基础有限，必须开发核心设施，这些设施提供无法通过商业实体购买的服务。这无疑适用于流动和细胞分类，因为没有其他设施能够在北达科他州整个州进行分类。同样，通过商业实体或远离家庭实验室的学术中心进行流动，即使不是不可能的话，也很困难。对于细胞的分类尤其如此，在大多数情况下，需要立即加工和/或产品培养物。这种情况的一个主要例子在于干细胞的尖端技术，如果没有能力将所得细胞进行培养和/或分析。 No Inbre已将这一核心确定为在No中进一步发展，教学和服务的必要。简而言之，在本科生职业的某个关头，每个注定要进入研究生或卫生专业人士的本科生都应该暴露于流量和细胞分类。对于专业的博士后奖学金和医学生的研究生也是如此。