HLS-Scanning small laser beam flow cytometry:New concepts for microparticle analysis

HLS-扫描小激光束流式细胞仪：微粒分析的新概念

• 批准号: 8980640
• 负责人: Masanobu Yamamoto
• 金额: $ 22.91万
• 依托单位: CYTOMICS ANALYTICAL, LLC
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8980640
• 关键词: Biological; Biology; Blood; Blood Cells; Blood specimen; Caliber; Calibration; Cells; Color; Confocal Microscopy; Cytometry; Data; Data Set; Data Sources; Detection; Development; Devices; Electronics; Flow Cytometry; Fluorescence; Gold; HL-60 Cells; HL60; Hela Cells; Hematology; Human Cell Line; Image; Imaging Device; Immunology; Industry; Joints; Label; Laboratories; Lasers; Lead; Liquid substance; Marketing; Measures; Medical; Microfluidics; Microscopy; Music; Noise; Optics; Pathology; Pathway interactions; Plasma; Population; Property; Reading; Resolution; Sampling; Scanning; Science; Side; Signal Transduction; Source; Speed; Stream; System; Technology; Testing; Time; Titrations; Urine; Whole Blood; Work; Writing; base; cost; design; detector; high throughput screening; innovation; instrument; nanosized; new technology; next generation; novel diagnostics; particle; physical separation; public health relevance; screening; tool

 DESCRIPTION (provided by applicant): Flow cytometry has been a core technology in biological and medical sciences for nearly 50 years having been initiated by Mack Fulwyler in 1965 and driven by the Herzenberg laboratory into the field of immunology as well as many other fields. However the core principles have changed little over that time. While the limits of the technology has advanced from 1 to a current maximum of 18 fluorescent colors and two scatter signals, the greater majority of instruments operate somewhere in the lower-middle of this technology range. The fundamental principles of the technology have remained the same over this time and only measures total cellular signals based on each cell as it passes the detection point. This provides a rich data source, but with low content with regard to each parameter collected. Each cell has a single value for the entire cell for each variable collected. The contrast with a technology such as confocal microscopy is clear. The latter produces tremendous data content, but mostly based on a fewer cells with inability to operate on suspended cells-the key value of flow cytometry. Separating cells using cellular properties to obtain population information is difficult in imaging, and physical separation is virtually impossible. It is true that high content screening systems can collect a lot of data on a lot of cells, but the manipulation of those populations is not as sophisticated as multiparameter flow cytometry. The present innovation produces both high content and high cell data streams but importantly with significant increase in resolution of small particles because of the scanning technology. Our approach is generated by many years of leading edge development of disk reading and writing technologies at the highest possible resolution. DVD discs for example have pits in the 10 nm range. By transferring some of these ideas onto a microfluidic system using laser diameters at similar sizes used in Blu-ray we have opened up an entirely new opportunity and have defined this as micro-scanning imaging confocal flow cytometry. The result is a tremendously rich data content on cells flowing at approximately 1 m/s with a scan rate of 1MHz to achieve very high resolution data. A major opportunity now exists to rewrite the information collected from blood cells and in particular microparticles which are too small to be analyzed by current flow cytometers. When realized, the proposed technology will be able to collect many channels of fluorescence as well as detailed morphological data on tiny particles opening up a new chapter in advanced micro particle analysis in whole blood or plasma as well as other biological samples.

 描述：流式赛体是Mack Fulwyler在1965年被IT 50年的核心技术，由Herzenberg实验室驱动到免疫学领域以及许多其他核心原则该技术的限制已从1个荧光室RS和两个散射信号提升到当前的最大值，大多数仪器在该技术范围的下层中都有某种操作。基于每个单元格，这是一个富含的数据源，但与每个参数的含量相对于整个参数巨大的数据含量，但主要基于在悬浮细胞上操作的较少的细胞 - 流式细胞仪的关键值。精致的多参数流式化计师。生产者在扫描技术的分辨率上很重要，并且在磁盘读取技术的分辨率上很重要。在蓝光中使用的类似尺寸，我们开放了一个新的机会统一性，微扫描成像蛋白流式赛cyotitry。为了重写来自血细胞的信息，尤其是在实现时，提出的技术将是许多荧光渠道，以及有关微小S开放全血或血浆中高级微粒分析的新章节的细节数据以及其他生物学样本。