Rapid, High Yield, Single-Tube Nucleic Acid Extraction at Low Temperature using Lateral Ultrasonic Wave Transducer

使用横向超声波换能器在低温下快速、高产、单管核酸提取

基本信息

批准号：
9046543
负责人：
Poonam Sansanwal
金额：
$ 15万
依托单位：
MICROSONIC SYSTEMS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2017-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9046543
关键词：
Area Behavioral Medicine Biological Assay Biological Markers Biology Breast Chronic Kidney Failure Connective Tissue Cytolysis DNA Detection Development Device or Instrument Development Devices Diagnosis Evaluation Fluorescence Freezing Goals Health Care Costs Healthcare Industry Histocompatibility Testing Hospital Departments Human Kidney Lateral Liver Mediating Medical Genetics Molecular Diagnosis Muscle Myocardium Nucleic Acids Patients Phase Polymerase Chain Reaction Process Prostate Protocols documentation RNA Sampling Skeletal Muscle Skin Skin Tissue Striated Muscles System Techniques Technology Temperature Testing Therapeutic Thyroid Diseases Thyroid Gland Time Tissue Sample Tissues Transducers Tube Tumor Tissue Ultrasonic wave Ultrasonics Ultrasonography Workload cancer biomarkers cold temperature driving force genetic analysis human tissue improved instrument magnetic beads nucleic acid detection oncology personalized medicine point of care point-of-care diagnostics public health relevance qubit tool tumor tumor heterogeneity

Area Behavioral Medicine Biological Assay Biological Markers Biology Breast Chronic Kidney Failure Connective Tissue Cytolysis DNA Detection Development Device or Instrument Development Devices Diagnosis Evaluation Fluorescence Freezing Goals Health Care Costs Healthcare Industry Histocompatibility Testing Hospital Departments Human Kidney Lateral Liver Mediating Medical Genetics Molecular Diagnosis Muscle Myocardium Nucleic Acids Patients Phase Polymerase Chain Reaction Process Prostate Protocols documentation RNA Sampling Skeletal Muscle Skin Skin Tissue Striated Muscles System Techniques Technology Temperature Testing Therapeutic Thyroid Diseases Thyroid Gland Time Tissue Sample Tissues Transducers Tube Tumor Tissue Ultrasonic wave Ultrasonics Ultrasonography Workload cancer biomarkers cold temperature driving force genetic analysis human tissue improved instrument magnetic beads nucleic acid detection oncology personalized medicine point of care point-of-care diagnostics public health relevance qubit tool tumor tumor heterogeneity

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项目摘要

DESCRIPTION (provided by applicant): Genetic analysis is a driving force behind "Personalized Medicine" and has become an essential tool in advancing all areas of biology, medicine, and behavioral biology. As medical genetic sample analysis technology continues to develop there will eventually be a shift from the old paradigm of sending the sample out to the lab, to point-of-care sample analysis. Microsonic Systems Inc. proposes to develop a rapid portable sample prep device for nucleic acid analysis using our Bulk Lateral Ultrasonic (BLU) Wave Transducer for point-of-care diagnostics. Key to this instrument development will be the ability to rapidly perform multiple steps of nucleic acid sample prep in a single tube at cold (<25 C.) temperatures. Phase I specific aims include: A) optimization of the BLU energy parameters and nucleic acid extraction (NAE) protocol using normal frozen human muscle and skin tissues; and B) to evaluate and validate the optimized BLU energy nucleic acid extraction (NAE) protocol across normal and tumor samples from multiple human tissue types (e.g., breast, kidney, prostate, liver and thyroid). Development of a point-of-care portable instrument (Phase II) to aid in detection/treatment of oncology associated with these tissue groups is a potential paradigm shifting concept for the health care industry.

描述（由适用提供）：遗传分析是“个性化医学”背后的推动力，它已成为推进生物学，医学和行为生物学领域的重要工具。随着医学遗传样本分析技术的不断发展，最终将从将样品发送到实验室的旧范式转变为护理点样本分析。 Microsonic Systems Inc.建议使用我们的大量外侧超声波（BLU）波传感器开发快速便携式样品准备设备进行核酸分析，以进行护理点诊断。这种仪器开发的关键将是能够在寒冷的单个管中快速执行核酸样品准备的多个步骤（<25 C。）温度。第一阶段的特定目的包括：a）使用正常冷冻的人类肌肉和皮肤组织优化BLU能量参数和核酸提取（NAE）方案； b）跨多种人组织类型（例如乳腺癌，肾脏，前列腺，肝脏和甲状腺）的正常和肿瘤样品进行评估和验证优化的BLU能量核酸提取（NAE）方案。开发一种护理便携式工具（II阶段），以帮助检测与这些组织组相关的肿瘤学治疗是医疗保健行业的潜在范式转移概念。