HOST-PATHOGEN INTERACTION CORE

宿主-病原体相互作用核心

• 批准号: 9115190
• 负责人: George A. O'Toole
• 金额: $ 14.79万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9115190
• 关键词: Advisory Committees; Back; Basic Science; Biological Models; Biology; Cause of Death; Cell Culture Techniques; Cell Line; Cells; Centers of Research Excellence; Chronic; Coculture Techniques; Development; Doctor of Philosophy; Electrophysiology (science); Epithelial Cells; Human; Immunology; Industry; Infection; Instruction; Lead; Lung; Lung diseases; Microbial Biofilms; Microbiology; Mission; Molecular; Pathogenesis; Pharmacotherapy; Pilot Projects; Pseudomonas aeruginosa; Research; Research Personnel; Respiratory Tract Infections; Services; Small Interfering RNA; System; Techniques; Translational Research; Work; experience; medical schools; meetings; novel; novel therapeutics; pathogen; professor; response

PROJECT SUMMARY (See instructions): The mission of the Host-Pathogen Interaction Core (HPIC) is to advance basic science, as well as translational and discovery studies relevant to lung disease. A central service of this Core is to provide expertise using a co-culture platform of bacterial biofilms on human airway cells developed at the Geisel School of Medicine at Dartmouth. This Core also provides a range of airway cell lines, as well as primary human airway epithelial cells from CF and non-CF donors, to support studies assessing host-pathogen interactions of relevance to the lung. This Core facilitates ongoing studies of COBRE Lung Biology Center (LBC) investigators and the pilot studies supported via this COBRE 111 application. The notable accomplishments of the HPIC include: 1) Development of a novel co-culture model system; 2) Assistance with a wide range of host-pathogen interaction studies by providing expertise in cell culture, siRNA and other molecular techniques, and electrophysiology studies; and 3) Coordinating with industry to develop new therapeutics aimed at chronic infections of the lung. The HPIC was launched in 2010 in response to a growing need by COBRE LBC investigators for support in crosscutting studies at the interface of bacterial pathogenesis and the host airway. We leveraged a set of earlier findings from the Stanton and O'Toole labs, in particular the study of bacterial biofilms on airway epithelial cells and host-pathogen interactions, to build a critical set of novel capabilities that have been used extensively by LBC investigators since the inception of this core. The HPIC was developed to meet the unique research needs of LBC investigators and received approval and strong backing from the External Advisory Committee, who deemed it critical to meet the needs of the evolving LBC. This Core is directed by George OToole, Ph.D., Professor of Microbiology and Immunology. Dr. OToole has over 20 years of experience in bacterial systems, and since 2000 has worked closely with Dr. Bruce Stanton, COBRE Director and PI, on a variety of projects that directly resulted in the development of the central capabilities of this Core.

项目摘要（请参阅说明）：宿主 - 病原体相互作用核心（HPIC）的使命是进步基础科学，以及与肺部疾病有关的转化和发现研究。该核心的中心服务是使用在达特茅斯（Geisel）医学院开发的人类气道细胞上使用细菌生物膜的共同培养平台提供专业知识。该核心还提供了一系列气道细胞系，以及来自CF和非CF供体的原代人气道上皮细胞，以支持评估与肺部相关性宿主 - 病原体相互作用的研究。该核心促进了对山核桃肺生物学中心（LBC）研究者的正在进行的研究，以及通过此Cobre 111应用支持的试点研究。 HPIC的显着成就包括：1）开发新型共培养模型系统； 2）通过在细胞培养，siRNA和其他分子技术以及电生理学研究方面提供专业知识来帮助进行广泛的宿主 - 病原体相互作用研究； 3）与行业协调开发针对肺部长期感染的新治疗剂。 HPIC于2010年启动，以应对Cobre LBC研究者在细菌发病机理和宿主气道界面上的横切研究方面的支持。我们利用Stanton和O'Toole Labs的一系列早期发现，尤其是对气道上皮细胞和宿主 - 病原体相互作用的细菌生物膜的研究，以建立一组关键的新型功能，自从该核心启动以来，LBC研究者已广泛使用，这些功能已被LBC研究者广泛使用。 HPIC的开发是为了满足LBC调查人员的独特研究需求，并获得了外部咨询委员会的认可和强烈支持，后者认为满足不断发展的LBC的需求至关重要。该核心由微生物学和免疫学教授乔治·奥托尔（George Otoole，Ph.D.）指导。 Otoole博士在细菌系统方面拥有20多年的经验，自2000年以来，与Cobre Director and Pi与Bruce Stanton博士紧密合作，从事各种项目，这些项目直接导致了该核心核心能力的发展。