Functional Visual Core (Core D)

功能视觉核心（核心D）

• 批准号: 9056018
• 负责人: SAMANTHA J BUTLER
• 金额: $ 22.28万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9056018
• 关键词: Affect; Animal Model; Animals; Basic Science; Biological; Biological Models; Biological Process; Brain; Brain region; Cell Culture Techniques; Cell physiology; Cells; Cellular biology; Center for Translational Science Activities; Communities; Complex; Confocal Microscopy; Coupled; Data; Development; Developmental Disabilities; Disease; Fluorescence Microscopy; Gene Expression; Generations; Genetic; Goals; Human; Image; Image Analysis; Imagery; Imaging technology; Implant; In Vitro; Laboratories; Life; Light Microscope; Lipids; Magnetic Resonance Imaging; Mental Retardation and Developmental Disabilities Research Centers; Methodology; Methods; Microscope; Microscopy; Modality; Modeling; Molecular Probes; Mus; Nervous System Physiology; Neurons; Organoids; Patients; Pattern; Photons; Population; Process; Protocols documentation; Reagent; Recording of previous events; Refractory; Research; Research Personnel; Research Support; Resolution; Resources; Rodent; Rodent Model; Role; Sampling; Scientist; Services; Spectrum Analysis; Stem Cell Research; Stem cells; Structure; Structure of thyroid parafollicular cell; Syndrome; System; Techniques; Therapeutic Intervention; Tissues; Translating; Translational Research; Visual; Visual Acuity; Work; abstracting; animal imaging; brain circuitry; cellular imaging; cognitive neuroscience; fluorescence microscope; holistic approach; imaging system; in vivo; indexing; innovation; light microscopy; meetings; member; miniaturize; nervous system development; neural circuit; neurodevelopment; new technology; novel; open source; optical imaging; optogenetics; relating to nervous system; submicron; translational neuroscience

CORE D: Abstract The foremost objective of the Structural and Functional Visualization Core is to provide state of the art imaging services to the IDDRC community, including both conventional and confocal microscopy, whole animal MRI acquisition and analysis support. To attain this goal, the core has been redesigned to provide comprehensive imaging services for IDDRC researchers working at on any aspect of the genetic and environmentally-induced developmental diseases affecting nervous system development and function. The research ongoing in the IDDRC spans basic scientists using reductionist approaches to elucidate the mechanisms specific to intellectual developmental disabilities (IDDs) to clinicians assessing therapeutic interventions for patients. To accommodate all of their imaging requirements, we are now providing access to three light microscopy cores, including a microscopy suite dedicated to IDDRC researchers, two MRI facilities and the technical support needed to initiate and complete any imaging analysis. Many IDDRC researchers have imaging systems within their own laboratories, however they are often insufficient to meet their demand and are never comprehensive. Being able to access the IDDRC-supported microscopy cores thus both adds capacity and provides technical resources not otherwise available, permitting researchers to refocus their studies in novel and innovative directions. A second key objective of the Structural and Functional Visualization Core is to develop new technologies for visualizing biological samples and in turn provide them to IDDRC researchers. Here, we focus on [1] developing smaller lighter one-photon miniaturized fluorescent microscopes for live imaging neural activity in freely moving animals (1-3) and [2] refining the methods for CLARITY, a protocol that renders tissue transparent (4, 5), thereby permitting unparalleled visual acuity into the complex circuitry of the brain. These techniques offer the promise of a holistic approach to cutting edge imaging, permitting IDDRC researchers to translate mechanism into therapy. For example, researchers investigating a specific intellectual disorder, such as Dup15q syndrome (see model project), will be able perform MRI on patients to identify the affected region of the brain, implant miniaturized microscopes in rodent models to perform in vivo i m a g i n g to examine how the firing patterns of specific populations of neuron are mechanistically altered by the disease, while concomitantly examining putative aberrant circuit formation using light microscopy coupled with CLARITY. Finally, this core also supports the efforts of all the other cores, offering IDDRC researchers the ability to both probe molecular and cellular function at any level from the sub-cellular to living animals and determine the consequence of therapeutic interventions.

核心D：摘要 结构和功能可视化核心的最大目标是提供最新的状态 IDDRC社区的成像服务，包括常规和共聚焦显微镜，整个 动物MRI获取和分析支持。为了实现这一目标，重新设计了核心以提供 IDDRC研究人员在遗传和遗传方面工作的全面成像服务 影响神经系统发育和功能的环境引起的发育疾病。这 IDDRC正在进行的研究跨越基础科学家，采用还原主义的方法来阐明 针对临床医生评估治疗的智力发育障碍（IDD）的机制（IDD） 患者的干预措施。为了满足他们所有的成像要求，我们现在正在提供访问 至三个光显微镜核，包括专用于IDDRC研究人员的显微镜套件，两个MRI 设施和启动和完成任何成像分析所需的技术支持。许多IDDRC 研究人员在自己的实验室内拥有成像系统，但是他们通常不足以满足 他们的需求，从不全面。能够访问IDDRC支持的显微镜核心 因此，这两者都增加了能力并提供了其他其他可用的技术资源，从而使研究人员允许 将他们的研究重新集中在新颖和创新的方向上。 结构和功能可视化核心的第二个关键目标是开发新的 可视化生物样品并将其提供给IDDRC研究人员的技术。在这里，我们 专注于[1]开发用于实时成像的较小较小的单光量微型荧光显微镜 自由移动动物的神经活动（1-3）和[2]完善了清晰的方法，该方案是 使组织透明（4，5），从而允许无与伦比的视力进入复杂的电路 大脑。这些技术提供了一种整体方法的前沿成像的希望，允许 IDDRC研究人员将机制转化为治疗。例如，研究特定的研究人员 智力障碍，例如DUP15Q综合征（请参阅模型项目），可以对患者进行MRI 识别受影响的地区 大脑，植入物在啮齿动物模型中的微型显微镜在体内执行，以检查如何检查 神经元特定种群的发射模式在机械上被疾病改变了，而 同时使用光学显微镜和清晰的光学显微镜检查假定的异常电路形成。 最后，该核心还支持所有其他核心的努力，为IDDRC研究人员提供 能够从亚细胞到活动物的任何水平探测分子和细胞功能 并确定治疗干预措施的结果。