Mutant Mouse Core

突变小鼠核心

• 批准号: 9003078
• 负责人: EDWARD G. SHESELY
• 金额: $ 33.07万
• 依托单位: HENRY FORD HEALTH SYSTEM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9003078
• 关键词: AGTR2 gene; Angiotensin II Receptor; Animal Husbandry; Animals; Area; Breeding; Budgets; Cardiac Myocytes; Chickens; Core Facility; Duct (organ) structure; EP4 receptor; Ear; Epithelial; Gene Targeting; Generations; Genes; Genotype; Hypertension; Institution; Instruction; Investigation; Knock-out; Knockout Mice; LoxP-flanked allele; Luciferases; Maintenance; Mouse Strains; Mus; Mutant Strains Mice; Mutation; NOS3 gene; Organ; Ovalbumin; Pathogenesis; Principal Investigator; Prostaglandins; Receptor, Angiotensin, Type 1; Research Personnel; Services; Sodium Channel; Source; Syndrome; T-Cell Receptor; Transgenes; Transgenic Mice; Type 2 Angiotensin II Receptor; WFDC2 gene; Weaning; collecting tubule structure; epithelial Na+ channel; experience; gain of function; homologous recombination; mouse model; programs; research study; routine care

The Mutant Mouse Core will centralize effort and provide the expertise necessary to maintain stocks of mice with various mutations generated by gene targeting and/or transgene insertion for use by participating investigators. Investigators requiring mice with specific targeted mutations and/or transgenes will be able to obtain the appropriate genotypes in requisite numbers from the Core. The Core is thus responsible for the routine care of founder and breeding animals, the husbandry required to generate the experimental animals, their weaning, application of ear tags for identification, and obtaining genotypes for the relevant gene(s). The Core is also responsible for supplying suitable controls when they are not readily commercially available. Breeding programs will be carried out in coordination with each investigator to obtain the appropriate number of animals of the required genotypes. We will provide the investigators in the PPG renewal the following gene knockout mice; Angiotensin 1 receptor type l a "floxed" (ATIa-floxed) -/-, angiotensin II receptor type 2 (AT2) -/Y, endothelial nitric oxide synthase (Nos3 or eNOS) -/-, conditional knockouts of the a epithelial sodium channel (ENaC) in both the cortical collecting duct (CCD) alone and the CCD plus the connecting tubule (CNT), and cardiac myocyte specific knockout of the prostaglandin EP4 receptor (CS-EP4) -/-. We will provide the following transgenic mice: NF-KB/luciferase (NF-KB-LUC) Tg, and chicken ovalbumin specific a and p T-cell receptor (OT II) Tg mice. In addition the Core will provide mice, generated by homologous recombination, which have a gain-of-function in the PENaC gene as a mouse model of Liddle's syndrome. In addition the Core will provide the following strains, directly purchased as needed as experimental animals to PPG investigators; Ragl -/-, p47-/-, NZBWF1/J, and NZW/LacJ. RELEVANCE (See instructions): The Mutant Mouse Core of the PPG, Core C will provide genetically modified mice to support the investigations to be conducted in Projects l-IV, as described in those sections. Providing these core services will allow the investigators to concentrate on their experiments without having to concern themselves with the day-to-day husbandry and genotyping of mice.

突变的鼠标核心将集中努力并提供维持小鼠库存所需的专业知识 基因靶向和/或转基因插入产生的各种突变供参与 调查人员。需要具有特定靶向突变和/或转基因的小鼠的调查人员将能够 从核心中获取必要数字的适当基因型。因此，核心负责 创始人和繁殖动物的常规护理，生成实验动物所需的饲养， 它们的断奶，用于识别的ear标签的应用以及获得相关基因的基因型。这 核心还负责提供合适的控件时，当它们不易市售时。 育种计划将与每个研究人员协调，以获取适当的数字 所需基因型的动物。我们将在PPG续约中为调查人员提供以下内容 基因敲除小鼠；血管紧张素1受体型L a“ floxed”（原-氟） - / - ，血管紧张素II受体2型 （AT2） - /Y，内皮一氧化氮合酶（NOS3或ENOS） - / - ，A上皮的条件敲除 单独的皮质收集管（CCD）和CCD加上连接的钠通道（ENAC） 前列腺素EP4受体（CS-EP4）的小管（CNT）和心肌细胞特异性敲除 - / - 。我们 将提供以下转基因小鼠：NF-KB/荧光素酶（NF-KB-LUC）TG和鸡卵形蛋白特异性 A和P T细胞受体（OT II）TG小鼠。此外，核心将提供由同源物产生的小鼠 重组，在PENAC基因中具有功能获得，作为Liddle综合征的小鼠模型。在 核心将提供以下菌株，根据需要直接购买作为实验动物 PPG调查人员； ragl - / - ，p47 - / - ，nzbwf1/j和nzw/lacj。 相关性（请参阅说明）： PPG的突变小鼠核心核心C将提供转基因的小鼠以支持 如这些部分所述，在L-IV项目中进行的调查。提供这些核心服务 将允许调查人员专注于他们的实验，而不必关心 小鼠的日常饲养和基因分型。