Cell Bio/Imaging Core

细胞生物/成像核心

• 批准号: 9145706
• 负责人: Larry R. Gerace
• 金额: $ 39.84万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9145706
• 关键词: Behavior; Biological Assay; Cell physiology; Cells; Cellular Structures; Cellular biology; Collaborations; Complex; Complex Mixtures; Computer Simulation; Confocal Microscopy; Electron Microscope; HIV; In Vitro; Life; Light Microscope; Multiprotein Complexes; Nuclear Lamina; Nucleocytoplasmic Transport Proteins; Nucleoproteins; Protein Analysis; Proteomics; Research Infrastructure; Research Institute; Technical Expertise; Techniques; Technology; Time; base; bioimaging; cellular imaging; in vivo; instrumentation; light microscopy; macromolecule; mathematical analysis; microscopic imaging; nucleocytoplasmic transport; protein complex; screening; small molecule inhibitor; structural biology; trafficking

Cell Biology and Imaging Core (Gerace. Director: Ambrus-Aikelin. Manager) Overview The Cell Biology and Imaging Core provides access to state-of-the-art facilities and technologies for light and electron microscope imaging of macromolecules in fixed and living cells, proteomic analysis of complex mixtures of proteins, and analysis of cell structure and function by various in vitro and in vivo techniques. Thematic focus and technical expertise includes nucleocytoplasmic transport of proteins and nucleoprotein complexes, dynamics of multiprotein complexes of cells such as the nuclear lamina, and screening for small molecule inhibitors of nuclear transport using cell-based assays. The infrastructure of the center includes collaborations with Drs. Gaudenz Danuser and John Yates at the Scripps Research Institute. Gaudenz Danuser is a leading expert in mathematical analysis and computational modeling of the dynamic behavior of cellular components, and provides access to instrumentation for spinning disc confocal microscopy for quantitative real-time light microscopy. John Yates provides access to highthroughput proteomics for analysis of complex protein fractions.

细胞生物学和成像核心（Gerace。主管：Ambrus-Aikelin。Manager）概述细胞生物学和成像核心的访问提供了对固定和活细胞中大分子的光和电子显微镜成像的最先进的设施和技术，并访问了蛋白质混合物的蛋白质混合物，以及VITO和VITO的蛋白质混合物的复杂混合物，并在VITO和VITO中进行分析。主题焦点和技术专长包括蛋白质和核蛋白复合物的核质转运，核层中细胞多蛋白复合物的动力学，以及使用基于细胞的测定的核转运小分子抑制剂筛选。该中心的基础架构包括与DRS的合作。 Scripps研究所的Gaudenz Danuser和John Yates。 Gaudenz Danuser是细胞组件动态行为的数学分析和计算模型领域的领先专家，并为旋转碟片共聚焦显微镜提供了仪器的访问，以进行定量实时光学显微镜。约翰·耶茨（John Yates）提供了对高通量蛋白质组学的访问，以分析复杂的蛋白质分数。