I-Corps: Assisting in the commercialization of the Atoptix fiber based holographic CARS microscope

I-Corps：协助基于 Atoptix 光纤的全息 CARS 显微镜的商业化

基本信息

批准号：
9194183
负责人：
Perry Edwards
金额：
$ 4万
依托单位：
ATOPTIX, LLC
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-01-01 至 2017-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Coherent Raman microscopy has emerged as an important label-free imaging modality, which can enable long duration non-invasive imaging of cells and tissues. However, current coherent Raman systems require scanning to obtain three-dimensional images, hindering studies of rapid dynamic biological processes. Additionally, coherent Raman imaging systems usually rely on complex and expensive laser sources, which limit their accessibility. In this Phase I SBIR study, Atoptix, LLC. proposes to develop a fiber-based holographic coherent anti-Stokes Raman scattering (CARS) microscope to overcome these limitations of scanning and system complexity. The key innovation, CARS holography, merges the unique capabilities of CARS spectroscopy and holographic imaging by recording of a digital CARS hologram which captures both the amplitude and the phase of an anti-Stokes image signal. CARS holography can thus perform label-free, 3D imaging without scanning, and have an improved sensitivity over conventional CARS due to its homodyne detection nature. Furthermore, the proposed system will use a robust, turn-key fiber laser based excitation system which overcomes the complexities and high costs associated with existing coherent Raman systems. To perform this work, Atoptix will collaborate with Penn State University researchers who are the inventors of CARS holography. Specific Aim 1 is to develop a synchronized two-color ultrafast fiber laser system that will provide the CARS pump/probe and Stokes excitation sources. Specific Aim 2 is to develop the prototype holographic CARS microscope. The system will be characterized and optimized to assess performance and capability for Phase II development of an advanced prototype system.

描述（由申请人提供）：相干拉曼显微镜已成为一种重要的无标记成像方式，它可以实现细胞和组织的长时间非侵入性成像。然而，当前的相干拉曼系统需要扫描才能获得三维图像，阻碍了快速动态生物过程的研究。此外，相干拉曼成像系统通常依赖于复杂且昂贵的激光源，这限制了其可访问性。在这项 I 期 SBIR 研究中，Atoptix, LLC。建议开发一种基于光纤的全息相干反斯托克斯拉曼散射（CARS）显微镜，以克服扫描和系统复杂性的这些限制。关键的创新是 CARS 全息术，通过记录数字 CARS 全息图，捕获反斯托克斯图像信号的幅度和相位，融合了 CARS 光谱学和全息成像的独特功能。因此，CARS 全息术可以在无需扫描的情况下执行无标记 3D 成像，并且由于其零差检测性质，其灵敏度比传统 CARS 更高。此外，所提出的系统将使用强大的、交钥匙光纤激光器激发系统，该系统克服了与现有相干拉曼系统相关的复杂性和高成本。为了完成这项工作，Atoptix 将与 CARS 全息术的发明者、宾夕法尼亚州立大学的研究人员合作。具体目标 1 是开发同步双色超快光纤激光系统，该系统将提供 CARS 泵浦/探针和斯托克斯激发源。具体目标 2 是开发原型全息 CARS 显微镜。该系统将被表征和优化，以评估先进原型系统第二阶段开发的性能和能力。