I-Corps: Assisting in the commercialization of the Atoptix fiber based holographic CARS microscope

I-Corps：协助基于 Atoptix 光纤的全息 CARS 显微镜的商业化

基本信息

批准号：
9194183
负责人：
Perry Edwards
金额：
$ 4万
依托单位：
ATOPTIX, LLC
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-01-01 至 2017-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Coherent Raman microscopy has emerged as an important label-free imaging modality, which can enable long duration non-invasive imaging of cells and tissues. However, current coherent Raman systems require scanning to obtain three-dimensional images, hindering studies of rapid dynamic biological processes. Additionally, coherent Raman imaging systems usually rely on complex and expensive laser sources, which limit their accessibility. In this Phase I SBIR study, Atoptix, LLC. proposes to develop a fiber-based holographic coherent anti-Stokes Raman scattering (CARS) microscope to overcome these limitations of scanning and system complexity. The key innovation, CARS holography, merges the unique capabilities of CARS spectroscopy and holographic imaging by recording of a digital CARS hologram which captures both the amplitude and the phase of an anti-Stokes image signal. CARS holography can thus perform label-free, 3D imaging without scanning, and have an improved sensitivity over conventional CARS due to its homodyne detection nature. Furthermore, the proposed system will use a robust, turn-key fiber laser based excitation system which overcomes the complexities and high costs associated with existing coherent Raman systems. To perform this work, Atoptix will collaborate with Penn State University researchers who are the inventors of CARS holography. Specific Aim 1 is to develop a synchronized two-color ultrafast fiber laser system that will provide the CARS pump/probe and Stokes excitation sources. Specific Aim 2 is to develop the prototype holographic CARS microscope. The system will be characterized and optimized to assess performance and capability for Phase II development of an advanced prototype system.

描述（由申请人提供）：连贯的拉曼显微镜已成为一种重要的无标签成像模态，可以使长时间的持续时间对细胞和组织的无创成像进行。但是，当前的相干拉曼系统需要扫描才能获得三维图像，这阻碍了快速动态生物过程的研究。此外，连贯的拉曼成像系统通常依赖于复杂且昂贵的激光源，从而限制了它们的可访问性。在此阶段I SBIR研究中，Atoptix，LLC。提议开发基于纤维的全息相干抗螺旋体拉曼散射（CARS）显微镜，以克服这些扫描和系统复杂性的局限性。关键的创新（CARS全息图）通过记录数字汽车全息图，融合了汽车光谱和全息成像的独特功能，该全息图既捕获了反stokes图像信号的振幅和阶段。因此，汽车全息图可以执行无标签的3D成像，而无需扫描，并且由于其同源性检测性质而对常规汽车具有提高的灵敏度。此外，提出的系统将使用强大的，键纤维激光的激发系统，以克服与现有相干拉曼系统相关的复杂性和高成本。为了执行这项工作，Atoptix将与宾夕法尼亚州立大学的研究人员合作，这些研究人员是汽车全息图的发明者。具体目的1是开发同步的两色超快激光系统，该系统将提供汽车泵/探针并刺激激发源。具体目标2是开发原型全息汽车显微镜。该系统将经过特征和优化，以评估高级原型系统II期开发的性能和能力。