Protein Interact. Core

蛋白质相互作用。

• 批准号: 9145699
• 负责人: Debra Muir Eckert
• 金额: $ 13.28万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9145699
• 关键词: Area; Binding; Biological; Biosensor; Buffers; Complex; Computer software; Core Facility; Data Analyses; Development; Drops; Electronic Mail; Ensure; Equipment; Funding; Gel; Gel Chromatography; Grant; HIV; Health Sciences; Human Resources; Label; Laboratories; Lipids; Measurement; Measures; Molecular Weight; Oligonucleotides; Optics; Process; Property; Proteins; Proteome; Qualifying; Research Personnel; Research Project Grants; Resolution; Sampling; Schedule; Shapes; Signal Transduction; Surface; Surface Plasmon Resonance; System; Technology; Thermodynamics; Time; United States National Institutes of Health; Universities; Utah; Work; analytical ultracentrifugation; base; cofactor; design; imaging system; instrument; light scattering; macromolecular assembly; macromolecule; medical schools; meetings; protein protein interaction; research study; sedimentation equilibrium; sedimentation velocity; sensor; small molecule; square foot; stoichiometry; structural biology; trafficking

Protein Interactions Core (Mvszka. Director) Overview Our Protein Interaction Core will tap into an existing Core facility that is part of the University of Utah's Health Sciences Core facilities. The Protein Interaction Core facility was established in 1996 to provide internal and external academic users access to state-of-the-art, label-free, real-time interaction technologies. Over the past 10 years, this facility has contributed to more than 100 research projects that encompass a wide array of biological areas. The Protein Interaction Core is centrally located within the School of Medicine (Rm 4A416) on the Health Sciences campus. The laboratory's 1600 square feet of space was entirely renovated in 1997 and was specifically designed to accommodate the Core facility's equipment and personnel. Currently, the facility maintains 7 surface plasmon resonance-based optical biosensors: 3 Biacore 2000s, 1 Biacore 3000, 1 Biacore S51, 1 Biacore Flexchip, and 1 Lumera Proteome Processor. The Biacore 2000/3000 platforms are ideal for measuring protein-protein interactions, while the Biacore S51 is designed for small-molecule interaction analysis. The Flexchip and Proteome Processor instruments are array-based imaging systems that allow one to study up to 1000 binding partners immobilized onto the sensor surface at one time. The Core has worked closely with both Biacore and Lumera on the development of this new hardware, as well as data analysis software. In early 2007, we plan to add a new biosensor (the ProteOn XPR36 from BioRad Laboratories) to our Core, funded by ab NIH Shared Instrument Grant. Together, these state-of-the-art instruments allow us to measure the interactions of biomolecules such as proteins, oligonucleotides, and lipids in high-resolution, as well as high-throughput, formats. Experiments are scheduled through email contacts with the Core's manager. Typically, the investigator and their staff then meet with the manager to plan the best course of action for the proposed study. Samples are then dropped off at the Core and experiments are initiated the same day. This ensures that labile samples are able to be processed in a timely manner.

蛋白质相互作用的核心（MVSZKA。Derare）概述我们的蛋白质相互作用核心将利用现有的核心设施，该设施是犹他大学健康科学核心设施的一部分。蛋白质相互作用的核心设施成立于1996年，旨在为内部和外部学术用户访问最先进的，无标签的实时互动技术。在过去的10年中，该设施为100多个研究项目做出了贡献，这些研究项目涵盖了各种各样的生物领域。蛋白质相互作用的核心位于医学科学校园的医学院（RM 4A416）内。该实验室的1600平方英尺空间在1997年进行了完全翻新，专门设计用于容纳核心设施的设备和人员。目前，该设施维持7个基于等离子体共振的光学生物传感器：3 Biacore 2000s，1 Biacore 3000、1 Biacore S51、1 Biacore Flexchip和1个Lumera Proteome处理器。 BIACORE 2000/3000平台是测量蛋白质蛋白质相互作用的理想选择，而Biacore S51则设计用于小分子相互作用分析。 Flexchip和Proteome处理器仪器是基于阵列的成像系统，使一个人最多可以研究1000个结合伙伴一次固定在传感器表面上。核心与Biacore和Lumera紧密合作，以开发这种新硬件以及数据分析软件。在2007年初，我们计划将新的生物传感器（来自Biorad Laboratories的Proteon XPR36）添加到我们的核心，并由AB NIH共享工具赠款资助。这些最先进的仪器共同使我们能够测量高分辨率中蛋白质，寡核苷酸和脂质等生物分子的相互作用，以及高通量的格式。实验是通过与Core经理的电子邮件联系人来安排的。通常，调查员及其工作人员会与经理会面，以计划拟议研究的最佳行动方案。然后将样品放在核心上，并在同一天开始实验。这样可以确保能够及时处理不稳定的样本。