MR Imaging of Bioscaffold-Induced Neural Progenitor Migration

生物支架诱导的神经祖细胞迁移的磁共振成像

基本信息

批准号：
10273779
负责人：
Michel M. Modo
金额：
$ 41.44万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-01 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10273779
关键词：
Adult Anatomy Brain Cells Characteristics Complex Contrast Media Control Groups Data Date of birth Detection Development Engineering Ensure Extracellular Matrix Fluorescence Microscopy Gadolinium Generations Histologic Histology Hydrogels Imaging Device Immunohistochemistry Implant Infarction Injections Injury Invaded Label Lateral Magnetic Resonance Imaging Mammals Maps Methods Modification Natural regeneration Neurons Noise Outcome Play Process Regenerative Medicine Resolution Role Route Signal Transduction Site Source Specificity Speed Stroke Testing Time Tissues Up-Regulation Visualization adult neurogenesis analytical tool bioscaffold brain cell brain tissue cell motility design experimental study histological studies implantation improved in vivo in vivo imaging insight lateral ventricle migration nanoGold nerve stem cell neurogenesis novel novel strategies preimplantation relating to nervous system response spatiotemporal stem cells subventricular zone tissue regeneration tool

项目摘要

Abstract The regeneration of brain tissue has been considered one of the greatest challenges for regenerative medicine. Implantation of an extracellular matrix (ECM) hydrogel can produce de novo brain tissue by attracting endogenous neural progenitors into a tissue cavity. These neural progenitors originate in the subventricular zone (SVZ), one of the few neurogenic sites in the adult brain. Initially, neural progenitors migrate in the peri-infarct- damaged tissue before invading the bioscaffold. However, little is known about the route or time-course of migration of these cells. The objective of this application is to develop a non-invasive MR imaging paradigm that affords the tracking of neural progenitors from the SVZ to the bioscaffold. Specifically, we here aim to: 1) develop and optimize contrast agent to label endogenous neural progenitor cells, 2) to non-invasively track the migration of neural progenitors and 3) to determine the temporal dynamics of neural progenitors responding to a hydrogel implant. Achieving an in vivo visualization of neural progenitor migrations will aid us to understand the contribution of neurogenesis to brain tissue regeneration, but also to devise novel strategies that can manipulate this process.

抽象的脑组织的再生被认为是人类面临的最大挑战之一再生医学。植入细胞外基质（ECM）水凝胶可以通过将内源性神经祖细胞吸引到组织中来从头产生脑组织空腔。这些神经祖细胞起源于室下区（SVZ），是少数神经祖细胞之一成人大脑中的神经源位点。最初，神经祖细胞在梗塞周围迁移在侵入生物支架之前损坏组织。然而，人们对这条路线知之甚少或这些细胞迁移的时间过程。该应用程序的目标是开发一个非侵入性 MR 成像范例，可跟踪来自神经祖细胞的神经祖细胞 SVZ 到生物支架。具体来说，我们的目标是：1）开发和优化造影剂标记内源性神经祖细胞，2）非侵入性追踪神经祖细胞的迁移神经祖细胞和3)确定神经祖细胞的时间动态对水凝胶植入物做出反应。实现神经祖细胞的体内可视化迁移将帮助我们了解神经发生对脑组织的贡献再生，而且还设计出可以操纵这一过程的新策略。