Label-free optical imaging for human mesoscale connectivity with a focus on deep brain stimulation targets

用于人体中尺度连接的无标记光学成像，重点关注深部脑刺激目标

基本信息

批准号：
10586107
负责人：
TANER AKKIN
金额：
$ 49.71万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-03-07 至 2027-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10586107
关键词：
3-Dimensional Algorithms Anatomy Animal Model Architecture Atlases Axon Birefringence Brain Brain Diseases Brain Mapping Brain imaging Cells Deep Brain Stimulation Development Diffusion Magnetic Resonance Imaging Disease Dissection Electroencephalography Electron Microscopy Face Fascicle Fiber Foundations Functional Magnetic Resonance Imaging Future Generations Goals Histologic Human Image Imaging technology Injections Internal Capsule Label Lasers Lateral Location Macaca mulatta Maps Mental disorders Methods Microscopic Motor Motor Cortex Neurons Optical Coherence Tomography Optics Pathway interactions Pattern Performance Phase Population Rattus Resolution Sampling Structure Structure of subthalamic nucleus Synapses System Techniques Technology Thalamic structure Tissues Tracer Visible Radiation Visualization Work analytical tool attenuation brain circuitry brain tissue cingulate cortex connectome density falls gray matter human imaging image processing imaging modality improved in vivo meter multiparametric imaging nanoscale nervous system disorder neural tract neuroimaging neurosurgery nonhuman primate novel optical imaging polarized light quantitative imaging reconstruction targeted treatment tractography white matter

项目摘要

PROJECT SUMMARY AND ABSTRACT Many neurological and psychiatric disorders are essentially connectionist disorders: certain sets of neurons have abnormally increased or decreased connectivity with other sets of neurons. Deep brain stimulation (DBS) therapies target small, unique populations of axons and/or cell bodies in order to treat brain disorders and normalize connectivity. Thus, mapping the wiring diagram of the brain is an important goal. Macroscale connectivity has been studied indirectly in humans using noninvasive neuroimaging. In order to develop a much higher resolution connectivity map of the brain, this project will develop depth-resolved polarized light imaging to visualize axons and fiber tracts. Since brain imaging and mapping at microscopic resolution is feasible with intrinsic optical contrasts (e.g. polarization-based) and depth-resolved block-face imaging is desired before histological processing, we have developed the serial optical coherence scanner (SOCS) for large-scale or whole brain imaging with microscopic resolution. SOCS combines a polarization-maintaining fiber based polarization-sensitive optical coherence tomography and a tissue slicer. This project will create a novel SOCS system that can image axonal tracts at the micron scale spatial resolution using unbiased optical contrasts (Aim 1). The approach will be evaluated, refined, and compared in the same brain tissue to neural tract-tracer labeling of tracts associated with DBS targets for brain disorders, in nonhuman animal models (Aim 2). The approach will then be applied to DBS targets in the human brain (Aim 3). The physical scales at which this project investigates the brain microstructure are unique (1-10 μm resolution across centimeters of tissue). This project will pave the way for the foundation of a future human connectome at the micron scale, which is the highest resolution achievable with current optical technology for imaging an entire human brain.

项目概要和摘要许多神经系统和精神疾病本质上是联结障碍：某些神经元组与其他神经元组的连接异常增加或减少。（DBS）疗法针对小而独特的轴突和/或细胞体群体，以治疗脑部疾病因此，绘制大脑的接线图是一个重要的目标。为了开发一种连接性，人们使用非侵入性神经成像技术对人类进行了间接研究。更高分辨率的大脑连接图，该项目将开发深度分辨偏振光成像以可视化轴突和纤维束，因为微观分辨率的大脑成像和绘图是很重要的。可能具有固有光学对比度（例如基于偏振）和深度分辨块面成像在组织学处理之前，我们开发了串行光学相干扫描仪 (SOCS) 具有显微分辨率的大规模或全脑成像结合了偏振保持。该项目将创建一个基于光纤的偏振敏感光学相干断层扫描和组织切片机。新型 SOCS 系统，可以使用无偏光学以微米级空间分辨率对轴突束进行成像对比（目标 1）该方法将在相同的脑组织和神经组织中进行评估、改进和比较。在非人类动物模型中，与 DBS 脑部疾病目标相关的纤维束示踪剂标记（目标 2）然后该方法将应用于人脑中的 DBS 目标（目标 3）。该项目研究的大脑微观结构是独特的（跨厘米的分辨率为 1-10 μm）该项目将为未来微米级人类连接组的基础铺平道路，这是当前光学技术对整个人脑成像所能达到的最高分辨率。