Linking Synaptic and Cognitive Deficits in a Model of Neuropsychiatric Disease
将神经精神疾病模型中的突触和认知缺陷联系起来
基本信息
- 批准号:9069064
- 负责人:
- 金额:$ 23.41万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-09-20 至 2018-01-31
- 项目状态:已结题
- 来源:
- 关键词:AcuteAddressAdhesionsAfferent PathwaysAttentionAutistic DisorderBehaviorBehavioralBehavioral ParadigmBrainBrain regionCell Adhesion MoleculesCognitiveCognitive deficitsCommunicationComplexCorpus striatum structureDRD2 geneDataDefectDetectionDiseaseDorsalElectrophysiology (science)EnvironmentEtiologyExcitatory SynapseFocal AdhesionsFunctional disorderFutureGene TargetingGenesGeneticGenetic studyGlutamate ReceptorGoalsGrantHippocampus (Brain)HumanHuman GeneticsIndividualInjection of therapeutic agentInterventionKnowledgeLaboratory AnimalsLearningLeucine-Rich RepeatLigandsLightLinkLiteratureLong-Term DepressionMaintenanceMedialMediatingMediator of activation proteinMentorsMethodologyModelingMolecular AnalysisMusMutant Strains MiceMutationNeuronsNucleus AccumbensObsessive-Compulsive DisorderOutputPathogenesisPatientsPhasePhenotypePoint MutationPopulationPrefrontal CortexRecruitment ActivityReversal LearningRoleScaffolding ProteinSchizophreniaSignal TransductionSliceSynapsesSynaptic TransmissionSynaptic plasticityTechniquesTertiary Protein StructureTestingTrainingViralWorkbaseburden of illnesscell typecognitive functioncognitive rigiditycostexecutive functionflexibilitygenetic associationgenetic linkageimprovedinterestmutantneural circuitneuropsychiatric disorderoptogeneticspostsynapticsynaptic functionsynaptogenesistransmission processtreatment strategy
项目摘要
Recent evidence suggests that abnormalities in synaptic transmission may underlie the pathogenesis of
several neuropsychiatric disorders. A better understanding of normal and aberrant synaptic function may
provide improved detection and treatment strategies, thereby reducing overall disease burden. Towards these
ends, the use of gene targeting in mouse has provided a unique opportunity to create laboratory animals with
similar mutations to human patients that have clinically defined neuropsychiatric disorders. Thorough analysis
of these mutant mice, both at the behavioral and cellular level, can thus provide a wealth of information about
potential mechanisms of disease formation as well as the relationship between gene dysfunction and abnormal
behavior. Evidence from a variety of human genetic association studies has highlighted the functional
relevance of synaptic cell adhesion molecules (SCAMs) in neuropsychiatric disorders including autism,
schizophrenia and obsessive compulsive disorder. The Neuroligin (NL)/Neurexin complex, a prototypical
SCAM pair, has been implicated in excitatory and inhibitory synaptic function. In an attempt to further explore
NL function, a mouse was made with a point mutation (R451C) in the NL3 gene to mimic a mutation
associated with autism in a human genetics study. The goal of this proposal is to study this mutant mouse
from both behavioral and synaptic perspectives, with the hope of linking abnormal behaviors to specific
underlying circuit and synaptic abnormalities. Causality will then be established through attempts to ameliorate
behavioral phenotypes by addressing the underlying synaptic deficits. I have chosen to focus on functional
cognitive deficits, particularly cognitive flexibility, in NL3R451C mutants, as these represent a core, debilitating
feature of many neuropsychiatric disorders and their underlying synaptic mechanisms have thus far received
little attention. Preliminary data suggest that NL3R451C mutants have severely impaired cognitive flexibility.
During the mentored phase, I will employ viral injections to localize which synapses require NL3 function to
maintain cognitive flexibility. In addition, I will employ optogenetic recruitment of cortical afferent populations in
the acute slice to explore abnormalities of corticostriatal synaptic transmission in the dorsal striatum of
NL3R451C mutant mice. For the independent phase, I will first explore potential abnormalities in synaptic
plasticity in NL3R451C mutants. After a thorough description of the alterations of both basal transmission and
activity-dependent plasticity found in the striatum of mutants, I will attempt to link these synaptic deficiencies to
the observed abnormalities in cognitive flexibility through the use of cell-type and regional optogenetic
manipulations. I anticipate that this proposal will uncover interesting information regarding the synaptic basis
of cognitive flexibility and how perturbations of synaptic transmission in NL3R451C mutants result in rigid
behavioral output. Furthermore, it will provide a template for my future studies into how other synaptic
molecules mediate cognitive function.
最近的证据表明,突触传播的异常可能是
几种神经精神疾病。更好地了解正常和异常的突触功能
提供改进的检测和治疗策略,从而减轻总体疾病负担。朝向这些
结束时,在鼠标中使用基因靶向的方法为创建实验室动物提供了独特的机会
与具有临床定义神经精神疾病的人类患者相似的突变。彻底的分析
这些突变小鼠在行为和细胞层面上都可以提供有关的大量信息
疾病形成的潜在机制以及基因功能障碍与异常之间的关系
行为。来自各种人类遗传关联研究的证据突出了功能
突触细胞粘附分子(SCAM)在神经精神疾病中的相关性,包括自闭症,
精神分裂症和强迫症。神经素(NL)/Neurexin复合物,一种原型
骗局对与兴奋性和抑制性突触功能有关。试图进一步探索
NL功能,在NL3基因中用点突变(R451C)制成小鼠以模拟突变
在人类遗传学研究中与自闭症相关。该提议的目的是研究这种突变鼠标
从行为和突触的角度来看,希望将异常行为与特定的行为联系起来
基础电路和突触异常。然后,将通过改善来建立因果关系
通过解决潜在的突触缺陷来解决行为表型。我选择专注于功能
NL3R451C突变体中的认知缺陷,尤其是认知灵活性,因为它们代表了核心,使人衰弱
到目前为止,许多神经精神疾病及其潜在的突触机制的特征已接收
很少关注。初步数据表明,NL3R451C突变体严重损害了认知灵活性。
在指导阶段,我将使用病毒注射来定位哪些突触需要NL3功能
保持认知灵活性。此外,我将在中遗传学募集皮质传入群体中
急性切片以探索在背纹状体中皮质纹状体突触传播异常
NL3R451C突变小鼠。对于独立阶段,我将首先探索突触的潜在异常
NL3R451C突变体中的可塑性。在彻底描述了基础传输和的改变之后
在突变体的纹状体中发现的活动依赖性可塑性,我将尝试将这些突触缺陷联系起来
通过使用细胞类型和局部光遗传学,观察到的认知灵活性异常
操纵。我预计该建议将发现有关突触基础的有趣信息
认知灵活性以及NL3R451C突变体中突触传播的扰动如何导致刚性
行为输出。此外,它将为我未来的研究提供一个模板,以了解其他突触
分子介导认知功能。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Marc V Fuccillo其他文献
Marc V Fuccillo的其他文献
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{{ truncateString('Marc V Fuccillo', 18)}}的其他基金
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Novel Role of a Ventral Striatal Circuit in Motor Control
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A Novel Role for Local Striatal Interneuron Regulation of Goal-Directed Action
局部纹状体中间神经元调节目标导向行动的新作用
- 批准号:
10338165 - 财政年份:2020
- 资助金额:
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A Novel Role for Local Striatal Interneuron Regulation of Goal-Directed Action
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- 批准号:
10558680 - 财政年份:2020
- 资助金额:
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Molecular and Circuit Mechanisms of Neurexin1-Mediated Goal-Directed Dysfunction
Neurexin1 介导的目标导向功能障碍的分子和电路机制
- 批准号:
10300008 - 财政年份:2017
- 资助金额:
$ 23.41万 - 项目类别:
Molecular and Circuit Mechanisms of Neurexin1-Mediated Goal-Directed Dysfunction
Neurexin1 介导的目标导向功能障碍的分子和电路机制
- 批准号:
10058775 - 财政年份:2017
- 资助金额:
$ 23.41万 - 项目类别:
Linking Synaptic and Cognitive Deficits in a Model of Neuropsychiatric Disease
将神经精神疾病模型中的突触和认知缺陷联系起来
- 批准号:
8547839 - 财政年份:2012
- 资助金额:
$ 23.41万 - 项目类别:
Linking Synaptic and Cognitive Deficits in a Model of Neuropsychiatric Disease
将神经精神疾病模型中的突触和认知缺陷联系起来
- 批准号:
8424086 - 财政年份:2012
- 资助金额:
$ 23.41万 - 项目类别:
Synaptic Analysis of Neuroligin1 function
Neuroligin1 功能的突触分析
- 批准号:
7676907 - 财政年份:2009
- 资助金额:
$ 23.41万 - 项目类别:
Synaptic Analysis of Neuroligin1 function
Neuroligin1 功能的突触分析
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7895499 - 财政年份:2009
- 资助金额:
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