Gene Targeting and Transgenic Facility

基因打靶和转基因设施

• 批准号: 9001959
• 负责人: MICHAEL J. WEBER
• 金额: $ 6.95万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9001959
• 关键词: Area; Biological Preservation; Cancer Center; Cancer Center Support Grant; Cancer Model; Clinic; Cryopreservation; Derivation procedure; Development; Drug resistance; Embryo; Epigenetic Process; Event; Evolution; Gene Targeting; Genetic; Genetically Engineered Mouse; Goals; Immune system; Instruction; Knock-in Mouse; Knock-out; Laboratories; Link; Malignant Neoplasms; Mission; Molecular; Mouse Strains; Mus; Mutation; Neoplasm Metastasis; Positioning Attribute; Production; Property; Research; Research Personnel; Resource Sharing; Resources; Services; Targeted Research; Technology; Training; Transgenic Organisms; Universities; University of Virginia Cancer Center; Virginia; anticancer research; cancer cell; design; embryonic stem cell; genetic analysis; genetic technology; human cancer mouse model; model design; mouse model; reconstitution; tool; tumor microenvironment; tumor progression

The University of Virginia Gene Targeting and Transgenic Facility (GTTF) is a Shared Resource that utilizes advanced transgenic and gene targeting technologies to efficiently produce and preserve genetically engineered mice for cancer model research for UVA Cancer Center investigators. The GTTF's mission is to support transgenic and gene targeting research endeavors, to provide efficient genetic technologies for germline and embryonic stem cell manipulations, and to serve as a resource for design, development and derivation of customized cancer-prone mouse strains. For the past five years, the GTTF has evolved into a unique shared resource that provides a range of integrated services covering five areas: (1) transgenic and knockout/knock-in mouse production, (2) gene targeting, (3) embryo cryopreservation/reconstitution, (4) mouse genetic analysis and (5) training. The GTTF is uniquely positioned to support cancer research. While cancer researchers have successfully studied the molecular and cellular properties of cancer cells propagated in culture, increasingly mouse models of cancer provide a more physiologically relevant opportunity to investigate the interplay of cancer cells, the tumor microenvironment, and the host immune system. Mouse models provide opportunities to study the evolution of cancer progression from first mutations to metastasis, and the unique opportunity to assess the genetic and epigenetic events correlated with acquisition of drug resistance. The overall goal of the GTTF is to provide the support with integrated service and training to make mouse model design, creation and preservation as seamless as possible for the benefit of researchers in the Cancer Center.

弗吉尼亚大学基因靶向和转基因设施（GTTF）是一种共享资源 利用先进的转基因和基因靶向技术有效地生产和保存遗传 为UVA癌症中心研究人员设计的癌症模型研究的小鼠。 GTTF的使命是 支持针对研究努力的转基因和基因，以提供有效的遗传技术 种系和胚胎干细胞操作，并作为设计，开发和 定制的容易发生小鼠菌株的推导。 在过去的五年中，GTTF已演变为独特的共享资源，提供了一系列 涵盖五个领域的集成服务：（1）转基因和敲除/敲除鼠标的产生，（2）基因 靶向，（3）胚胎冷冻保存/重构，（4）小鼠遗传分析和（5）训练。 GTTF 在支持癌症研究方面具有独特的位置。虽然癌症研究人员成功地研究了 在培养中传播的癌细胞的分子和细胞特性，越来越多的小鼠模型的癌症模型 提供了更具生理上相关的机会来研究癌细胞的相互作用，肿瘤 微环境和宿主免疫系统。鼠标模型提供了研究进化的机会 从初次突变到转移的癌症进展，以及评估遗传的独特机会 和表观遗传事件与耐药性的获取有关。 GTTF的总体目标是 为综合服务和培训提供支持，以使鼠标模型设计，创建和 尽可能无缝地保护癌症中心的研究人员。