Hypoxia induces SHMT2 to regulate cellular redox and epigenetics
缺氧诱导 SHMT2 调节细胞氧化还原和表观遗传学
基本信息
- 批准号:8821835
- 负责人:
- 金额:$ 10.44万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-08-05 至 2017-07-31
- 项目状态:已结题
- 来源:
- 关键词:3-phosphoglycerateAddressAffectBackBreast MelanomaCancer PatientCarbonCatabolismCell DeathCell Differentiation processCell ProliferationChromosomesConsumptionDNADNA MethylationDNA Modification MethylasesDataDiagnosisEnzymesEpigenetic ProcessEquilibriumGene Expression RegulationGenerationsGenesGenetic TranscriptionGlucoseGlutathioneGlutathione DisulfideGlycineGlycine HydroxymethyltransferaseHealthHistonesHomocysteineHomocystineHumanHypermethylationHypoxiaLinkMalignant NeoplasmsMammalian CellMetabolicMetabolic PathwayMetabolismMethylationMethylenetetrahydrofolate Dehydrogenase (NADP+)MethyltransferaseMitochondriaModificationNADPNon-Essential Amino AcidOncogenicOxidation-ReductionOxygenPathway interactionsPhosphoglycerate dehydrogenasePlayProductionProtein IsoformsRNA InterferenceRattusReactionReactive Oxygen SpeciesRegulationReportingRepressionResistanceRoleS-AdenosylhomocysteineS-AdenosylmethionineSeriesSerineSolid NeoplasmStressSubarachnoid HemorrhageTestingTetrahydrofolatesUp-RegulationWarburg EffectWithdrawalXenograft procedurealdehyde dehydrogenasesbasecancer cellcancer therapycancer typecarbenecofactorepigenetic regulationglucose productionhistone methylationhypoxia inducible factor 1insightmalignant breast neoplasmmethyl groupneuroblastoma cellnovelnovel therapeutic interventionoutcome forecastoverexpressionresearch studytargeted cancer therapytherapeutic targettranscription factortumortumor growthtumor microenvironmenttumor progressiontumorigenesis
项目摘要
DESCRIPTION (provided by applicant): An emerging hallmark of cancer is that malignant cells modulate metabolic pathways to promote cancer progression. Although a series of reports have demonstrated that the synthetic pathway of the non-essential amino acid serine is upregulated in cancer, it remains poorly understood that how serine catabolism contributes to cancer progression. My preliminary studies demonstrated that the mitochondrial enzyme serine hydroxymethyltransferase 2 (SHMT2) is overexpressed in many cancers via induction by a combination of hypoxia-inducible factors 1 (HIF-1) and Myc under hypoxia (low oxygen). Hypoxia is a common microenvironmental stress in solid tumor. Given that hypoxia contributes to tumor aggressiveness and resistance to cancer therapy, it is pressing to determine the possible role(s) of SHMT2 induction in tumor adaption to hypoxia. SHMT2 converts serine to glycine, with concurrent generation of one-carbon unit donor methylene-THF. I found that knockdown of SHMT2 enhanced cellular reactive oxygen species (ROS) level under hypoxia, indicating a novel role of SHMT2 in maintaining cellular redox balance upon hypoxic stress. In addition, repression of SHMT2 abolished DNA/histone hypermethylation stimulated by hypoxia, suggesting that one-carbon unit flux from SHMT2 may be critical for cellular methyltransferase activity, which contributes to epigenetic modification of chromosomes. Since Myc is required for the basal expression and hypoxic induction of SHMT2, it is critical to define the role of SHMT2-dependent DNA/histone hypermethylation in myc- dependent tumorigenesis. This enhanced methylation may correlate with the inability of neuroblastoma cells to activate lineage-specific genes involved in cellular differentiation. To address these issues, two Specific Aims are proposed: 1) Identify how hypoxia-induced SHMT2 regulates mitochondrial redox balance. 2) Define the role(s) of histone/DNA methylation regulated by SHMT2 under hypoxia. Through these proposed studies I hope to broaden the understanding of the metabolic regulation of redox control and epigenetic modification under hypoxia, and develop new therapeutic approaches targeting hypoxic cancer.
描述(由申请人提供):癌症的新出现标志是,恶性细胞调节代谢途径以促进癌症的进展。尽管一系列报道表明,非必需氨基酸丝氨酸的合成途径在癌症中上调,但丝氨酸分解代谢如何有助于癌症进展,仍然很少了解。我的初步研究表明,线粒体酶丝氨酸羟基甲基转移酶2(SHMT2)在许多癌症中通过低氧诱导因子1(HIF-1)(HIF-1)和低氧(低氧气)的组合通过诱导来过表达。缺氧是实体瘤中常见的微环境应激。鉴于缺氧有助于肿瘤侵袭性和对癌症治疗的抵抗力,因此迫切需要确定SHMT2诱导在肿瘤适应缺氧中的可能作用。 SHMT2将丝氨酸转换为甘氨酸,并同时生成一碳单位供体亚甲基-THF。我发现在缺氧下敲低SHMT2增强了细胞活性氧(ROS)水平,表明SHMT2在缺氧应激下保持细胞氧化还原平衡方面的新作用。此外,SHMT2的抑制消除了缺氧刺激刺激的DNA/组蛋白高甲基化,这表明来自SHMT2的单碳单位通量可能对细胞甲基转移酶活性至关重要,这有助于染色体的表观遗传修饰。由于MYC是SHMT2的基础表达和低氧诱导所必需的,因此定义SHMT2依赖性DNA/组蛋白高甲基化在MyC依赖性肿瘤发生中的作用至关重要。这种增强的甲基化可能与神经母细胞瘤细胞无法激活涉及细胞分化的谱系特异性基因有关。为了解决这些问题,提出了两个具体目标:1)确定缺氧诱导的SHMT2如何调节线粒体氧化还原平衡。 2)定义在缺氧下由SHMT2调节的组蛋白/DNA甲基化的作用。通过这些提出的研究,我希望扩大对缺氧下氧化还原控制和表观遗传修饰的代谢调节的理解,并开发针对低氧癌症的新治疗方法。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(1)
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{{ truncateString('Jiangbin Ye', 18)}}的其他基金
Hypoxia induces SHMT2 to regulate cellular redox and epigenetics
缺氧诱导 SHMT2 调节细胞氧化还原和表观遗传学
- 批准号:
9381095 - 财政年份:2015
- 资助金额:
$ 10.44万 - 项目类别:
Hypoxia induces SHMT2 to regulate cellular redox and epigenetics
缺氧诱导 SHMT2 调节细胞氧化还原和表观遗传学
- 批准号:
9120832 - 财政年份:2015
- 资助金额:
$ 10.44万 - 项目类别:
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