Comparative Medicine Infectious Diseases- Bethesda

比较医学传染病-贝塞斯达

基本信息

批准号：
8175466
负责人：
William Elkins
金额：
$ 1600.59万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8175466
关键词：
Affect Animal Model Animals Aortic Aneurysm Assisted Reproductive Technology Authorship Bioterrorism Breeding Caring Clinical Communicable Diseases Confounding Factors (Epidemiology)Cryopreservation DNA Data Diagnosis Disease Distress Electron Microscopy Environment Equipment Experimental Designs Gnotobiotic Histopathology Human Hypersensitivity Immunohistochemistry Inflammatory Response Knowledge Laboratory Animal Medicine Laboratory Animals Manuscripts Medicine Microinjections Mission Modification Mus National Center for Research Resources National Institute of Allergy and Infectious Disease Oranges Organism Pain Pathogenesis Pathology Preparation Principal Investigator Proteins Publications Pulmonary Alveolar Proteinosis Pulmonary alveolar structure Research Research Activity Research Personnel Research Support Resources Saguinus Services Specialist Technical Expertise Techniques Thrombosis Tissue Banks Transgenic Mice Veterinary Pathology Work abstracting assisted reproduction comparative cost design experience human disease medical specialties meetings posters programs research study surfactant

项目摘要

The CMB Infectious Disease Pathogenesis Section assisted Principal Investigators with experimental design, tissue collection and preparation, interpretation of histopathology findings, and assistance with manuscript preparation. CMB Research Support Specialists also provided technical expertise and support to NIAID Principal Investigators. Both pathology and technical proficiency resulted in numerous co-authorship opportunities. The CMB has undertaken the challenge of bringing a gnotobiotic facility on line and will initiate internal breeding. The CMB has an active Assisted Reproduction Technologies and Cryopreservation unit. This year the ARTiC group created a CAG-OFP mouse, a transgenic mouse with orange fluorescing protein in its DNA, by pronuclear microinjection. We found six moustached tamarins with a rare human disease called pulmonary alveolar proteinosis. This disease is characterized by the accumulation of surfactant in lung alveoli without generating an inflammatory response. No spontaneous animal model exist. Using immunohistochemistry and electron microscopy we confirmed the diagnosis of pulmonary alveolar proteinosis. The moustached tamarin could be the first spontaneous animal model for the study of this disease. We have submitted an abstract for a poster session at an NCRR meeting to be held this month on campus. We have also found spontaneous intracardiac thrombosis and aortic aneurysms in tamarins. A manuscript has been submitted to Comparative Medicine and is under review.

CMB感染性疾病发病机理部分协助首席研究人员对实验设计，组织收集和制备，组织病理学发现的解释以及手稿准备的帮助。 CMB研究支持专家还为NIAID首席研究人员提供了技术专长和支持。病理学和技术水平都带来了许多共同创作的机会。 CMB提出了将gnotobiotic设施置于线路并将启动内部育种的挑战。 CMB具有主动的辅助繁殖技术和冷冻保存单元。今年，ARTIC组创建了一种CAG OFP小鼠，这是一种通过核微注射的转基因小鼠，其DNA中具有橙色荧光蛋白。我们发现了六种含有一种罕见人类疾病的含肺瘤肺蛋白，称为肺肺泡蛋白质病。该疾病的特征是在肺肺泡中积累了表面活性剂，而不会产生炎症反应。没有自发动物模型。使用免疫组织化学和电子显微镜，我们证实了肺肺泡蛋白质病的诊断。探测的他氨酸可能是研究该疾病的第一个自发动物模型。我们已经在本月在校园举行的NCRR会议上提交了一个海报会议的摘要。我们还发现了他的菜他蛋白中自发的心脏内血栓形成和主动脉瘤。手稿已提交给比较医学，并正在审查中。