Fiber Inspired Neural Probes for the Multifunctional Dynamic Brain Mapping

用于多功能动态脑图绘制的纤维启发神经探针

基本信息

批准号：
8886012
负责人：
Polina O Anikeeva
金额：
$ 34.06万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-15 至 2019-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8886012
关键词：
Address Amygdaloid structure Anti-Inflammatory Agents Anti-inflammatory Appearance Behavior Behavioral Biological Assay Brain Brain Mapping Cell physiology Cells Chemicals Child Chronic Collaborations Complex Data Data Quality Decision Making Dependovirus Development Devices Dexamethasone Dimensions Drug Delivery Systems Electrodes Electronics Electrophysiology (science)Evolution Extinction (Psychology)Failure Farming environment Fiber Foreign Bodies Fright Future Generations Geometry Goals Histocompatibility Image Image Analysis Immunohistochemistry Implant Infusion procedures Institutes Ion Channel Knowledge Learning Letters Light Liquid substance Maps Measures Mechanics Medial Medical Memory Metals Methods Microfluidics Modeling Mus Neurons Neurosciences Noise Optics Performance Pharmaceutical Preparations Polymers Population Prefrontal Cortex Process Property Research Research Institute Resolution Signal Transduction Silicon Structure Techniques Technology Time Tissues Validation Viral Wild Type Mouse base cell type conditioned fear density design electric impedance flexibility implantation in vivo light transmission model building nervous system disorder neural stimulation novel optogenetics public health relevance relating to nervous system research study response

Address Amygdaloid structure Anti-Inflammatory Agents Anti-inflammatory Appearance Behavior Behavioral Biological Assay Brain Brain Mapping Cell physiology Cells Chemicals Child Chronic Collaborations Complex Data Data Quality Decision Making Dependovirus Development Devices Dexamethasone Dimensions Drug Delivery Systems Electrodes Electronics Electrophysiology (science)Evolution Extinction (Psychology)Failure Farming environment Fiber Foreign Bodies Fright Future Generations Geometry Goals Histocompatibility Image Image Analysis Immunohistochemistry Implant Infusion procedures Institutes Ion Channel Knowledge Learning Letters Light Liquid substance Maps Measures Mechanics Medial Medical Memory Metals Methods Microfluidics Modeling Mus Neurons Neurosciences Noise Optics Performance Pharmaceutical Preparations Polymers Population Prefrontal Cortex Process Property Research Research Institute Resolution Signal Transduction Silicon Structure Techniques Technology Time Tissues Validation Viral Wild Type Mouse base cell type conditioned fear density design electric impedance flexibility implantation in vivo light transmission model building nervous system disorder neural stimulation novel optogenetics public health relevance relating to nervous system research study response

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项目摘要

DESCRIPTION (provided by applicant): The development of the high-resolution cell-specific map of the neural activity associated with a particular behavior presents one of the major challenges in modern neuroscience. This dynamic electrophysiological mapping is particularly difficult in behaviors with a strong temporal variability, such as learning or memory. It is furthr aggravated by the limited long-term stability of the existing high-density electrophysiological platforms and the inability to uniquely identify cell types during recording. This project strivesto develop novel multi-functional fiber-inspired neural probes (FINPs) for long- term simultaneous neural recording and optogenetic and pharmacological cell-type identification. Specifically, we will combine soft polymer-based materials with a fiber-inspired fabrication process to create a platform that seamlessly integrates hundreds of micrometer-size electrodes, waveguides and drug delivery channels, while minimizing the potential tissue response to chronic implantation. We will characterize our structures with respect to their tissue compatibility and long-term functionality in chronic experiments in collaboration with Dr. William Shain in Seattle Children's Research Institute (SCRI), who will share his knowledge of histological methods and image analysis. Furthermore, the utility of the proposed FINPs will be evaluated in a basic neuroscience study relevant to learning. FINPs will be used to measure the changes in activity (mPFC) neurons that project to the basolateral amygdala (BLA) as a previously learned fear response is extinguished. The combined behavioral and electrophysiological experiments will be performed in close collaboration with Dr. Alla Karpova at the Janelia Farm Research Campus (JFRC), who will contribute her expertise in mPFC physiology and cell identification techniques.

描述（由适用提供）：与特定行为相关的神经元活动的高分辨率细胞特异性图的发展是现代神经科学中的主要挑战之一。在具有强大临时变异性（例如学习或记忆）的行为中，这种动态的电生理映射特别困难。现有高密度电生理平台的长期稳定性有限，并且无法在记录过程中唯一识别细胞类型，从而进一步汇总了它。该项目trivesto开发了新型的多功能纤维启发的神经问题（FINP），用于长期简单的神经元记录以及光学遗传学和药物细胞型鉴定。具体而言，我们将将柔软聚合物的材料与纤维启发的制造工艺相结合，以创建一个平台，该平台无缝整合了数百个微米大小的电子，波导和药物输送通道，同时最大程度地减少了对慢性植入的潜在组织反应。我们将在慢性实验中与西雅图儿童研究所（SCRI）合作的慢性实验中的组织兼容性和长期功能来表征我们的结构，后者将分享他对组织学方法和图像分析的了解。此外，将在与学习有关的基本神经科学研究中评估所提出的FINP的实用性。 FINP将用于衡量向基底外侧杏仁核（BLA）投射的活动（MPFC）神经元的变化，因为先前学到的恐惧反应已经消失。联合行为和电生理实验将与Janelia Farm Research Campus（JFRC）的Alla Karpova博士密切合作，后者将在MPFC生理学和细胞识别技术方面贡献她的专业知识。