Virus Imaging Core

病毒成像核心

• 批准号: 8337377
• 负责人: Thomas Hope
• 金额: $ 34.42万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8337377
• 关键词: Algorithms; Biomedical Research; Carbon Dioxide; Cells; Cellular Stress; Chicago; Computer software; Computers; Detection; Floor; HIV; Housing; Image; Laboratories; Life; Lighting; Microscope; Microscopy; Optics; Photobleaching; Photons; Phototoxicity; Process; Proteins; Resolution; Respiratory Diaphragm; Sampling; Signal Transduction; System; Universities; Viral; Virus; cellular imaging; digital; falls; image processing; instrument; lens; particle; photoactivation; photonics; red fluorescent protein; research study; structural biology; trafficking

Overview The laboratory for the Virus Imaging Core is currently located on the third floor of the Tarry Building on the Chicago Campus of Northwestern University. The laboratory will relocate to the state-of-the-art Lurie Biomedical Research Building in the fall of 2007. The Hope laboratory currently houses 3 DeltaVision RT deconvolution microscopes. Importantly, these microscopes are in a biocontained room located close to our HIV culture facility, allowing the safe observation of infectious HIV. Deconvolution microscopy uses computer processing to compensate for distortion caused by the optical path. Through the observation of fluorescent beads, an algorithm is developed which corrects the distortion caused by lenses and removes out-of-focus light163. The associated digital camera detects weak signals enabling great sensitivity and resolution, which is optimal for imaging viral particles in cells. Image capture with minimal illumination minimizes photobleaching and avoids phototoxicity, which can stress the cells during live cell observation. Two stand-alone workstations contain the complete Softworx software for deconvolutions and image processing, and off line image processing facilitates efficient instrument use. The systems are configured with filter sets allowing imaging of different types of fluorescent proteins including blue, cyan, green, yellow, and red fluorescent proteins. This permits simultaneous imaging of up to four different fluorescent proteins in live cells and 5 different fluors in fixed cell imaging. Importantly, two of these microscopes have environmental control chambers, including 5% CO2, allowing extended live cell timelapse microscopy. One of the microscopes with environmental control in equipped with a Photonics Mosaic digital diaphragm that allows precise sample illumination for photobleaching and photoactivation experiments. Additionally, this system has an ultrasensitive digital camera allowing maximal photon detection and signal amplification.

概述 病毒成像核心实验室目前位于塔里大楼的三楼 西北大学的芝加哥校园。实验室将搬到最先进的卢里 2007年秋天的生物医学研究大楼。希望实验室目前容纳3个三角处 RT反卷积显微镜。重要的是，这些显微镜位于近距离的生物室内房间中 向我们的艾滋病毒培养设施，可以安全地观察感染性艾滋病毒。反卷积显微镜 使用计算机处理来补偿由光路导致的失真。通过 观察荧光珠，开发了一种算法，该算法纠正了由 镜头和去除焦点的光163。相关的数码摄像头检测到弱信号 敏感性和分辨率很高，这是对细胞中病毒颗粒进行成像的最佳选择。图像捕获 最小的照明可最大程度地减少光漂白并避免光毒性，这会在 活细胞观察。两个独立的工作站包含完整的软件软件 反应和图像处理以及外部图像处理有助于有效的仪器使用。 系统配置了滤波集，允许对不同类型的荧光蛋白进行成像 包括蓝色，青色，绿色，黄色和红色荧光蛋白。这允许同时成像 在活细胞中，最多多种不同的荧光蛋白和固定细胞成像中的5种不同的荧光。 重要的是，其中两个显微镜具有环境控制室，包括5％CO2，允许 延长的活细胞时间解体显微镜。具有环境控制的显微镜之一 带有光子镶嵌数字膜片，可以精确的样品照明以进行光漂白和 光活化实验。此外，该系统具有超灵敏的数码相机，允许 最大光子检测和信号扩增。