Genetic Analysis of Hematopoietic Development

造血发育的遗传分析

基本信息

批准号：
7982449
负责人：
Shuo Lin
金额：
$ 9.9万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-01-07 至 2010-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7982449
关键词：
Angioblast Animal Model Anterior Antigens Arts Biological Biological Assay Blood Blood Vessels Cell Differentiation process Chimeric Proteins Comprehension Coupled DNA Binding Data Development Embryo Embryonic Development Endothelial Cells Enhancers Fishes Funding Gene Expression Gene Targeting Genes Genetic Genomics Goals Hematopoiesis Hematopoietic Hematopoietic stem cells Homologous Gene Human Laboratories Lateral Mesoderm MYB gene Mammals Mesoderm Mesoderm Cell Messenger RNA Molecular Profiling Mus Myelogenous Myeloid Cells Pathogenesis Pattern Physiology Process Proto-Oncogene Proteins c-myb Role Signal Transduction Stem cells Technology Testing Therapeutic Tissues Trans-Activators Transcript Transgenic Organisms Up-Regulation Vascular Diseases Zebrafish cis acting element design genetic analysis genome-wide human disease human embryonic stem cell migration novel novel therapeutic intervention overexpression progenitor public health relevance research study therapy development transcription factor vasculogenesis

项目摘要

DESCRIPTION (provided by applicant): Our goal is to understand the underlying mechanisms involved in regulating lineage specification of hematopoietic stem cells (HSC) and vascular angioblast cells from mesoderm during embryogenesis. Towards this goal we have chosen etsrp, one of the earliest lineage specific genes involved in hematopoietic and vascular development, as an entry point to dissect this process in zebrafish. Specifically, we will analyze functions of etsrp in definitive hematopoiesis, elucidate mechanisms regulating its specific expression, identify and characterize its biological targets. Since genes we propose to study are highly conserved between fish and mammals information obtained from zebrafish should be useful for understanding human pathogenesis and designing better therapeutics for hematological and vascular diseases. PUBLIC HEALTH RELEVANCE: Zebrafish is a popular model organism for studying development, physiology and human diseases. We use state of the art technologies to identify and characterize essential new genes required for the formation of blood stem cells and vascular progenitors in zebrafish. Since these genes are highly conserved between fish and human our studies will provide valuable information for understanding human hematopoietic and vascular diseases and assisting design of novel therapeutic approaches.

描述（由申请人提供）：我们的目标是了解调节造血干细胞（HSC）（HSC）和胚胎发生过程中中胚层血管细胞的谱系规范的潜在机制。为了实现这一目标，我们选择了ETSRP，这是涉及造血和血管发育的最早谱系特定基因之一，作为在斑马鱼中剖析这一过程的切入点。具体而言，我们将分析ETSRP在确定造血的功能，阐明调节其特定表达的机制，识别和表征其生物学靶标。由于我们建议研究的基因在从斑马鱼获得的鱼类和哺乳动物信息之间高度保守，对于理解人类的发病机理和为血液学和血管疾病设计更好的治疗剂应该有用。公共卫生相关性：斑马鱼是研究发展，生理和人类疾病的流行模型生物。我们使用艺术技术的状态来识别和表征斑马鱼中血管细胞和血管祖细胞形成所需的必需新基因。由于这些基因在鱼类和人类之间是高度保守的，我们的研究将提供有价值的信息，以了解人类造血和血管疾病，并有助于新型治疗方法的设计。