PROTEOMICS

蛋白质组学

• 批准号: 8121473
• 负责人: Jay William FOX
• 金额: $ 21.84万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8121473
• 关键词: Databases; Development; Funding; Genes; Goals; Immigration; Kinetics; Knowledge; Mass Spectrum Analysis; Measures; Methodology; Methods; Phase; Phosphorylation; Phosphorylation Site; Proteins; Proteomics; Technology; Variant; base; cell motility; gene discovery; migration; novel; protein complex

The overall goal of the Proteomics Initiative is to use mass spectrometry based proteomic methods to expand the current migration knowledge database by examining phosphorylation site utilization of proteins that regulate cell motility, by further developing methods for measuring differential phosphorylation of those proteins, and by using quantitative methods to assess positional and kinetic variation in phosphorylation site utilization in migration-related proteins. In addition, the Initiative will extend these proteomic methods to provide positional cartography of the proteins present in protein complexes formed by known migration-related proteins and protein products of novel migration genes identified in the unbiased screens of the Gene Discovery Initiative. These goals are enabled by several pivotal developments in technology, analysis, and methodology carried out during the first phase of funding. The Initiative focuses on two inter-related themes: 1. Phosphoproteomics of migration related proteins and 2. Positional proteomics of migration-related protein complexes and their phoshorylations.

蛋白质组学计划的总体目标是使用基于质谱的蛋白质组学 通过检查磷酸化站点利用的利用来扩展当前迁移知识数据库的方法 通过进一步开发用于测量差异磷酸化的方法来调节细胞运动的蛋白质 这些蛋白质，并使用定量方法评估磷酸化的位置和动力学变异 与迁移相关蛋白质中的位点利用。此外，该计划将将这些蛋白质组学方法扩展到 提供由已知迁移相关的蛋白质复合物中存在的蛋白质的位置制图 在基因的无偏筛选中鉴定出的新迁移基因的蛋白质和蛋白质产物 发现计划。这些目标是通过技术，分析和 在资金的第一阶段进行的方法论。该计划的重点是两个相互关联的主题：1。 迁移相关蛋白质和2。迁移相关蛋白的位置蛋白质组学的磷蛋白质组学 复合物及其幻影。