Biochemical Characteristics of the RecN Protein in DNA Repair Mechanisms

DNA修复机制中RecN蛋白的生化特性

基本信息

批准号：
8039919
负责人：
Emigdio D. Reyes
金额：
$ 2.84万
依托单位：
NEW MEXICO STATE UNIVERSITY LAS CRUCES
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-01 至 2012-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8039919
关键词：
ATP Hydrolysis Antineoplastic Agents Appearance Biochemical Biochemical Process Biological Biological Assay Cells Characteristics Chromosomes Complex DNA DNA Binding DNA Damage DNA Double Strand Break DNA Repair Deinococcus radiodurans Development Disease Double Strand Break Repair Enzymes Eukaryota Exhibits Family Genes Genetic Genome Goals Indium Individual Knowledge Lead Maintenance Malignant Neoplasms Methodology Molecular Organism Pathway interactions Pharmaceutical Preparations Phenotype Play Positioning Attribute Process Property Protein Family Proteins Public Health Radiation Radio Research Research Personnel Research Project Grants Resistance Resistance development Role Staging Structural Protein Techniques Testing Work bacterial resistance cancer cell cancer therapy cancer type career design improved insight interest irradiation member protein complex repaired therapy development

项目摘要

DESCRIPTION (provided by applicant): The appearance of resistant phenotypes in cancer cells hinders the successful treatment of cancer. Members of the Structural Maintenance of Chromosomes (SMC) protein family have been shown to contribute to resistant phenotypes in cancer cells by enhancing DNA repair. The goal of this research project is to understand the biochemical funcfion of RecN, a bacterial SMC protein, in the DNA repair mechanism of the radio-resistant bacterium Deinococcus radiodurans (DEIRA). To accomplish this goal, two aims have been developed. Aim 1 will describe the biochemical properties of RecN by testing the hypothesis that RecN is a DNA binding, ATP hydrolyzing protein that requires these biochemical acfivifies to facilitate the assembly of DNA fragments. Aim 2 focuses on determining the role of RecN in the biochemical processes implicated in the DNA double-strand break repair mechanism of DEIRA by tesfing the hypothesis that RecN facilitates the repair of DNA double-strand breaks by bridging DNA molecules together. A combinafion of genefic and biochemical methodology will be used to test our working hypotheses. These techniques include a spectrophotometric enzyme assay to assess ATP hydrolysis acfivity of RecN as well as various DNA binding techniques to examine the acfivity of this protein. This project is relevant to public health because the information obtained can provide basic knowledge about a group of proteins associated with the development of resistance to cancer therapy by cancer cells. The informafion acquired in this study can be use to improve the efficacy of cancer therapy.

描述（由申请人提供）：癌细胞中抗性表型的出现阻碍了癌症的成功治疗。染色体（SMC）蛋白质家族的结构维持的成员已显示通过增强DNA修复有助于癌细胞中的抗性表型。该研究项目的目的是了解抗遗传性细菌deinococcus radiodurans（DEIRA）的DNA修复机理RECN（一种细菌SMC蛋白）的生化功能。为了实现这一目标，已经开发了两个目标。 AIM 1将通过检验RECN是DNA结合的ATP水解蛋白的假设来描述RECN的生化特性，该假设需要这些生化效果以促进DNA片段的组装。 AIM 2的重点是确定RECN在DNA双链破裂修复机制中与DEIRA有关的生物化学过程中的作用，该假设通过将DNA DNA分子融合在一起，促进RECN促进DNA双链破裂的修复。 Genefic和生化方法的组合将用于检验我们的工作假设。这些技术包括分光光度酶测定法，以评估RECN的ATP水解效率以及各种DNA结合技术，以检查该蛋白质的效率。该项目与公共卫生有关，因为所获得的信息可以提供有关一组蛋白质与癌细胞对癌症治疗抗药性相关的蛋白质的基础知识。在这项研究中获得的信息可以用来提高癌症治疗的功效。