Prospective Study of Biofilms, Mycobacteria and Pseudomonads in Metalworking Flui

金属加工液中生物膜、分枝杆菌和假单胞菌的前瞻性研究

• 批准号: 7893038
• 负责人: Chuanwu Xi
• 金额: $ 19.31万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7893038
• 关键词: Prospective; Study; Biofilms; Mycobacteria; Pseudomonads

DESCRIPTION (provided by applicant): Each year, billions of gallons of metalworking fluids (MWFs) are consumed in manufacturing and a number of human health effects have been associated with exposure to MWFs, including various respiratory diseases, skin conditions, and cancers. Microbial contamination of MWFs has emerged as an important hazard to exposed workers. A newly identified organism, Mycobacterium immunogenum, appears to have an etiologic role in hypersensitivity pneumonitis (HP) in relation to metalworking fluids. However, our knowledge of this organism and the microbial ecology of MWFs in general is limited. Our preliminary studies reveal the wide-spread and high abundant presence of Mycobacteria in MWFs-associated biofilm samples from an automobile plant while no Mycobacteria were detected in some of the corresponding bulk fluid samples, which strongly support our hypothesis that biofilms are favorable sites for Mycobacteria to grow and survive in MWFs. In this exploratory/developmental project, we will prospectively characterize the microbial ecology of MWFs, with a specific focus on biofilms, Mycobacteria and Pseudomonads in metalworking fluids, and identify factors critical to the growth and survival of Mycobacteria in MWFs. Our specific aims are (1), Characterize microbial communities in MWFs and associated biofilms using molecular approaches; and (2), Monitor the prevalence and dynamics of Mycobacteria and Pseudomonads in MWFs using real-time PCR and correlate and identify parameters that contribute to the dynamics of Mycobacteria and Pseudomonads. . This proposed research will apply novel approaches (e.g., a major focus on biofilms associated with MWFs) and molecular techniques (e.g., 16S/18S rRNA clone library and real-time PCR) to better understand the overall microbial ecology of MWFs, and in particular, dynamics of Mycobacteria and Pseudomonads in MWFs. Statistical tools will be used to help to identify physical or chemical parameters that contribute to the growth of in Mycobacteria and/or Pseudomonads MWFs. The data obtained from this developmental project will enable future projects to develop novel approaches for the control of biofilm growth and Mycobacteria and Pseudomonads in MWFs. The outcome from this study and future projects will help users of MWFs to identify the right targets and develop novel approaches to monitor, control, reduce and/or prevent exposures to MWF-associated microbes that are suspected to cause adverse human health effects. Ultimately, this would serve to reduce the risk of adverse health events related to inhalation exposure to microbes or microbial products in MWFs. Public Health Relevance: We are combining both molecular methods and statistical tools for a prospective study of microbial ecology of metalworking fluids with a special focus on biofilms, Mycobacteria and Pseudomonads. The project responds up front to an immediate challenge to occupational health, i.e. hyposensitivity pneumonitis among workers exposed to Mycobacteria and Pseudomonads growing in metalworking fluids. Success of this project will have a great impact on occupational and public health.

描述（由申请人提供）：每年，制造中消耗了数十亿加仑的金属加工液（MWF），并且许多人类健康效应与接触MWF有关，包括各种呼吸道疾病，皮肤病和癌症。 MWF的微生物污染已成为对暴露工人的重要危害。与金属加工液有关，新近鉴定的有机体，分枝杆菌免疫原子似乎在超敏反应（HP）中具有病因。但是，我们对这种生物的了解和MWF的微生物生态学一般是有限的。我们的初步研究揭示了来自汽车植物的MWFS相关的生物膜样品中的分枝杆菌的广泛和大量存在，而在某些相应的散装液样品中未检测到分枝杆菌，这在我们的假设中强烈支持了生物膜是mycobactia for Mycobactia for Mard and Growive and growive and growive and Mwff seporive and Mwff s n of grows and Mwf。在这个探索性/发展项目中，我们将前瞻性地描述MWF的微生物生态学，特别关注金属加工液中的生物膜，分枝杆菌和假单胞菌，并确定对MWF中分枝杆菌生长和存活至关重要的因素。我们的具体目的是（1），使用分子方法来表征MWF和相关生物膜中的微生物群落； （2），使用实时PCR监测MWF中分枝杆菌和假单胞菌的患病率和动力学，并关联并识别有助于分枝杆菌和假单胞菌动态的参数。 。这项拟议的研究将采用新颖方法（例如，对与MWF相关的生物膜的主要关注）和分子技术（例如16S/18S rRNA克隆图书馆和实时PCR）更好地了解MWFS的整体微生物生态学，尤其是mycobacteria and PseudomonAds的动力学。统计工具将用于帮助识别有助于分枝杆菌和/或假单胞菌MWF的物理或化学参数。从这个发展项目中获得的数据将使未来的项目能够开发用于控制MWF中生物膜生长以及分枝杆菌和假单胞菌的新方法。这项研究和未来项目的结果将帮助MWF的用户确定正确的目标，并开发出新的方法来监测，控制，减少和/或防止对MWF相关的微生物的暴露，这些微生物被怀疑会引起不良人类健康的影响。最终，这将有助于降低与MWF中吸入的微生物或微生物产品有关的不良健康事件的风险。 公共卫生相关性：我们将分子方法和统计工具结合在一起，用于对金属加工流体的微生物生态学进行前瞻性研究，并特别关注生物膜，分枝杆菌和假单胞菌。该项目对职业健康的直接挑战做出了反应，即暴露于分枝​​杆菌和金属加工液中生长的分枝杆菌的工人中的性肺炎。该项目的成功将对职业和公共卫生产生重大影响。