Bacterial Diversity as a BioMarker of Soil Health

细菌多样性作为土壤健康的生物标志

基本信息

批准号：
7471371
负责人：
DANIEL BARTON OERTHER
金额：
$ 30.5万
依托单位：
UNIVERSITY OF CINCINNATI
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-08-01 至 2010-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7471371
关键词：
Address Affect Age Amendment Analytical Chemistry Anthracenes Aromatic Polycyclic Hydrocarbons Arsenic Bacteria Bacterial Genes Bacterial Model Bacterial Physiology Benzo(a)pyrene Bioavailable Bioinformatics Biological Assay Biological Availability Biological Markers Biology Biometry Bioreactors Bioremediations Biotechnology Cells Chemicals Cloning Coal Mining Collaborations Communities Complement component C1s Complex Complex Mixtures Condition Core Facility Custom DNA DNA analysis Data Analyses Diagnostic Diagnostic tests Discipline Dose Ecology Ecosystem Effectiveness End Point Environment Environmental Engineering technology Environmental Pollution Experimental Designs Exposure to Facility Construction Funding Category Food Chain Future Gene Expression Genes Genetic Transcription Genome Goals Growth Health Heavy Metals High Pressure Liquid Chromatography Human In Situ Individual Investigation Knowledge Lasers Libraries Link Measurement Measures Metabolism Metagenomics Metals Methods Microbial Biofilms Microscope Microscopy Modeling Molecular Biology Monitor Naphthalene Naphthalenes Nutrient Outcome Oxygenases Phylogenetic Analysis Pollution Polymerase Chain Reaction Population Primer Extension Principal Investigator Procedures Process Public Health Pyrenes Rate Recombinant DNA Research Research Personnel Residual state Resources Reverse Transcription Ribosomal DNA Ribosomal RNA Ribosomes Sampling Scanning Sentinel Serinus Site Soil Structure System Testing Time Validation Work anthracene anthropogenesis base bioaccumulation commercialization cost engineering design foot innovation instrument instrumentation mathematical model member microbial microbial community microorganism next generation novel phenanthrene pollutant programs pyrene remediation research study response sensor soil pollution success superfund site theories tool

项目摘要

Because of their intimate association with soil contaminants, we HYPOTHESIZE that the diversity [measured as richness and evenness) of the metabolically-active fraction of the soil bacterial community serves as a biomarker of the threat to human health from PAHs and heavy metals polluting Superfund sites. To challenge this hypothesis, we will address three SPECIFIC AIMS: (1) Identify correlations between the results of molecular biology-based measures of bacterial diversity and analytical measures of PAHs and heavy metals during soil ageing and bioremediation of mock Superfund sites; (2) Demonstrate novel molecular biology-based assays to assess the diversity of the metabolically active fraction of the bacterial community in situ; and (3) Demonstrate ecological models to predict soil bacterial community diversity under the influence of mixtures of heavy metals and PAHs. The APPROACH includes: (i) operating mock Superfund sites; (ii) T-RFLP and (iii) whole cell FISH to measure bacterial diversity; (iv) identifying predominant bacterial populations using clone libraries; (v) evaluating the impact of PAH and heavy metals on ribosome genesis; (vi) identifying heavy-metal responsive genes using microarrays; (vii) evaluating metagenomics of PAH degradation; (viii) evaluating real-time PCR to quantify heavy-metal and PAHresponsive genes; (ix) adapting ecological models to predict bacterial diversity; (x) comparing experimental measures of diversity with model predictions; and (xi) evaluating optimum nutrient amendments predicted with the resource-ratio theory. This project is INNOVATIVE because we are using molecular biology-based measures of bacterial diversity as a diagnostic tool to predict the threat to human health at Superfund sites (i.e., bacteria as sentinels for pollution). We expect that the positive findings of this study can be used to evaluate the potential for success of bioremediation strategies as well as to establish an effective endpoint of bioremediation (i.e., when appropriate bacterial diversity has been re-established and the threat to human health significantly reduced).

由于它们与土壤污染物密切相关，我们假设多样性 [以丰富度和均匀度衡量]土壤细菌群落的代谢活性部分作为多环芳烃和重金属污染超级基金场地对人类健康威胁的生物标志物。为了挑战这一假设，我们将解决三个具体目标：（1）确定基于分子生物学的细菌多样性测量结果和多环芳烃分析测量结果模拟超级基金场地土壤老化和生物修复过程中的重金属； (2) 展示小说基于分子生物学的测定来评估细菌代谢活性部分的多样性就地社区； (3) 展示预测土壤细菌群落多样性的生态模型重金属和多环芳烃混合物的影响。该方法包括： (i) 操作模拟超级基金网站； (ii) T-RFLP 和 (iii) 全细胞 FISH 来测量细菌多样性； (四) 识别使用克隆文库确定主要细菌种群； (v) 评估PAH和重金属的影响关于核糖体发生； (vi) 使用微阵列识别重金属响应基因； (七) 评估 PAH 降解的宏基因组学； (viii) 评估实时 PCR 来量化重金属和 PAH 响应基因； (ix) 采用生态模型来预测细菌多样性； (x) 比较实验通过模型预测来衡量多样性； (xi) 评估预测的最佳营养修正与资源比率理论。这个项目是创新的，因为我们正在使用基于分子生物学的细菌多样性测量作为诊断工具来预测超级基金地点对人类健康的威胁（即细菌作为污染的哨兵）。我们期望这项研究的积极结果可用于评估生物修复策略成功的潜力并建立有效的终点生物修复（即，当重新建立适当的细菌多样性并且对人类的威胁健康状况显着降低）。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Reverse transcription of 16S rRNA to monitor ribosome-synthesizing bacterial populations in the environment.

16S rRNA 的逆转录可监测环境中核糖体合成细菌种群。

DOI：
发表时间：
2009-07
期刊：
影响因子：
4.4
作者：
Lu, Ting;Stroot, Peter G;Oerther, Daniel B
通讯作者：
Oerther, Daniel B

Evaluation of swine-specific PCR assays used for fecal source tracking and analysis of molecular diversity of swine-specific "bacteroidales" populations.

评估用于猪特异性“细菌目”种群的粪便来源追踪和分子多样性分析的猪特异性 PCR 测定。

DOI：
发表时间：
2009-09
期刊：
影响因子：
4.4
作者：
Lamendella, Regina;Santo Domingo, Jorge W;Yannarell, Anthony C;Ghosh, Shreya;Di Giovanni, George;Mackie, Roderick I;Oerther, Daniel B
通讯作者：
Oerther, Daniel B

Identification of Naegleria fowleri in warm ground water aquifers.

温暖地下水含水层中福氏耐格里阿米巴的鉴定。