Interaction of Nutrient & Organochlorine Absorption

营养素的相互作用

• 批准号: 7792364
• 负责人: PATRICK TSO
• 金额: $ 32.16万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-03 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792364
• 关键词: Adipose tissue; Affect; Albumins; Animals; Binding; Biological; Blood; Blood Circulation; Body Weight decreased; Body fat; Brain; Cannulas; Cell Communication; Cell membrane; Chlorinated Hydrocarbons; Chylomicrons; Clinical; Country; Data; Dioxins; Disease; Enterocytes; Environment; Erythrocytes; Event; Excision; Excretory function; Fatty Acids; Fatty acid glycerol esters; Food Chain; Food Interactions; Funding; Future; Goals; Health; Hexachlorobenzene; Human; Human body; In Vitro; Incubated; Ingestion; Intestinal Absorption; Intestines; Label; Lead; Left; Light; Lipids; Lipolysis; Lipoproteins; Liposomes; Liver; Lymph; Lymphatic; Measurable; Measures; Membrane; Metabolism; Milk; Modification; NIH 3T3 Cells; Nature; Nutrient; Nutritional; Oral; Organ; Parents; Pattern; Pentachlorophenol; Phospholipids; Plankton; Plasma; Poison; Poisoning; Polychlorinated Biphenyls; Portal vein structure; Process; Proteins; Published Comment; Relative (related person); Reporting; Research; Research Personnel; Risk; Route; Surface; Therapeutic Intervention; Tissues; Triglycerides; Vesicle; Veterans; Vietnam; War; Weight Gain; absorption; agent orange; feeding; gastrointestinal absorption/transport; human tissue; in vivo; insight; lipophilicity; programs; research study; response

DESCRIPTION (provided by applicant): Organochlorine compounds (OCs) are toxic, carcinogenic, and ubiquitous in the environment. Oral ingestion is the principal route of entry into the human body for many OCs. Because of the lipophilicity of OCs and many of their metabolites, they are preferentially stored in adipose tissue, but are also found in liver, brain, and other organs and tissues. Although oral ingestion is the initial step in the accumulation of OCs in animals and humans, the processes involved in absorption from the intestine and early transport in blood and lymph are not well characterized. We propose studies that will shed light on these important steps in absorption and transport to provide a basic framework that can lead to understanding the ways that nutrients influence the absorption processes. In Specific Aim 1, we will determine the mode of absorption and association of OCs with carriers in lymph and the portal blood and whether this is affected by the amount and the type of lipid fed. We demonstrated that labeled hexachlorobenzene (HCB) carried in chylomicrons is cleared from the circulation faster than the labeled fatty acid (FA) as part of the triglyceride (TG). To explain this finding, the labeled HCB must not have been dissolved in the TG lipid core of the chylomicron. If it is, then its rate of removal from the circulation should be slower or at best equal to that of the labeled TG. Consequently, in Specific Aim 2, we will determine the mode of delivery of labeled OCs to the tissues (especially the adipose tissue) by proteins, phospholipids, and chylomicrons (CMs). We will also determine the partitioning of HCBs between chylomicrons and any membranes it comes in contact with e.g., red blood cell membrane. Lastly, in Specific Aim 3, we will determine if the relative importance of vehicle (albumin, lipid, or lipoprotein) carrying the labeled OC in blood is altered as a result of weight loss or weight gain (i.e. changes in body fat). The proposed research is important because of the biological and clinical importance of OCs in health and disease and they provide considerable new insights into how OCs are carried and delivered to the various organs in the body.

描述（由申请人提供）：有机氯化合物（OC）在环境中具有毒性，致癌性和无处不在。口服摄入是许多OC进入人体的主要途径。由于OC及其许多代谢产物的亲脂性，因此优先储存在脂肪组织中，但也发现在肝脏，脑，其他器官以及其他器官和组织中。尽管口服摄入是动物和人类OC积累的第一步，但血液和淋巴中肠道吸收和早期运输所涉及的过程并未得到很好的特征。我们提出的研究将阐明吸收和运输方面的这些重要步骤，以提供一个基本框架，以了解营养影响吸收过程的方式。在特定的目标1中，我们将确定OC与淋巴和门户血液中载体的吸收和关联方式，以及是否受脂质喂养的量和类型的影响。我们证明，作为甘油三酸酯（TG）的一部分，从循环中清除了在乳糖中携带的六氯苯（HCB）的标记。为了解释这一发现，标记的HCB一定不能溶解在乳糜微粒的TG脂质核中。如果是这样，则其从循环中的去除速率应较慢或最多等于标记的TG的速率。因此，在特定的目标2中，我们将通过蛋白质，磷脂和乳糜微粒（CMS）确定将标记的OC传递到组织（尤其是脂肪组织）的方式。我们还将确定HCB在乳糜微粒和任何与红色血细胞膜接触的膜之间的分配。最后，在特定的目标3中，我们将确定携带标记为OC在血液中携带的OC的车辆（白蛋白，脂质或脂蛋白）的相对重要性会因体重减轻或体重增加而改变（即体内脂肪的变化）。拟议的研究很重要，因为OC在健康和疾病中的生物学和临床重要性，并且对OCS的携带和交付方式提供了相当大的新见解。

项目成果

Feeding a Mixture of Choline Forms to Lactating Dams Improves the Development of the Immune System in Sprague-Dawley Rat Offspring.

向哺乳母鼠饲喂胆碱混合物可改善 Sprague-Dawley 大鼠后代免疫系统的发育。

• DOI: 10.3390/nu9060567
• 发表时间: 2017
• 期刊: Nutrients
• 影响因子: 5.9
• 作者: Richard,Caroline;Lewis,ErinD;Goruk,Susan;Wadge,Emily;Curtis,JonathanM;Jacobs,RenéL;Field,CatherineJ
• 通讯作者: Field,CatherineJ