Core Grant for Vision Research

视觉研究核心资助

基本信息

批准号：
8059623
负责人：
LINDA D HAZLETT
金额：
$ 33.78万
依托单位：
WAYNE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8059623
关键词：
American American Revolution Anatomy Animals Area Award Basic Science Cancer Center Support Grant Cancer Grant Supplements (P30)Cells Cellular biology Chairperson Cities Collaborations Complementary DNA Computer Workstations Computer software Country Cryopreservation Development Digital Photography Education Electron Microscopy Equipment Expenditure Extramural Activities Eye Faculty Foundations Funding Gel Grant Histopathology Housing Human Resources Image Image Analysis Immunofluorescence Immunologic Individual Institutes Institution Laboratory Personnel Lasers Learning Light Medical Medical Students Medical center Medicine Michigan Microscope Molecular Molecular Biology Names Ophthalmology Photography Pilot Projects Preparation Primary Cell Cultures Production Publications Research Research Project Grants Research Training Resolution Reverse Transcriptase Polymerase Chain Reaction Scanning Schools Scientist Strategic Planning Techniques Time Training United States National Institutes of Health Universities Vision Vision research bacterial vector base college cost digital digital imaging established cell line medical schools posters professor research facility tissue culture vision science

American American Revolution Anatomy Animals Area Award Basic Science Cancer Center Support Grant Cancer Grant Supplements (P30)Cells Cellular biology Chairperson Cities Collaborations Complementary DNA Computer Workstations Computer software Country Cryopreservation Development Digital Photography Education Electron Microscopy Equipment Expenditure Extramural Activities Eye Faculty Foundations Funding Gel Grant Histopathology Housing Human Resources Image Image Analysis Immunofluorescence Immunologic Individual Institutes Institution Laboratory Personnel Lasers Learning Light Medical Medical Students Medical center Medicine Michigan Microscope Molecular Molecular Biology Names Ophthalmology Photography Pilot Projects Preparation Primary Cell Cultures Production Publications Research Research Project Grants Research Training Resolution Reverse Transcriptase Polymerase Chain Reaction Scanning Schools Scientist Strategic Planning Techniques Time Training United States National Institutes of Health Universities Vision Vision research bacterial vector base college cost digital digital imaging established cell line medical schools posters professor research facility tissue culture vision science

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项目摘要

Continuing funds are requested by a group of 20 vision scientists and their laboratory personnel to support two research modules housed in the Department of Anatomy/Cell Biology that will continue to provide central units that will facilitate and enhance vision research at Wayne State and other participating Universities lacking core supported facilities. The modules will cost effectively enhance current research by providing this group of scientists with personnel and equipment, often not supported by individual R01 or other types of NEI grants, to advance NEI-funded research as a priority and in addition to provide opportunity for collaboration and for new pilot project development leading to increased NEI grant submissions. The two modules requested include: Imaging/Histopathology and Tissue Culture/Molecular. The Imaging/ Histopathology module provides for all facets of confocal laser scanning, light and electron microscopy, immunofluorescence, image analysis using metamorph imaging software, as well as training on microscopes and imaging software. There is assistance and training in the management of digital images, including digital graphics production, animal slit lamp photography, poster and publication production, digital photography equipment use, high resolution flatbed transparency scanning and imaging computer workstation usage within the module. The Tissue Culture/Molecular module provides assistance and training for preparation of specialized media, isolation, purification and characterization of ocular cells for primary culture, subculture and propagation of established cell lines, and cryopreservation of cells. Assistance and training is also available for fundamental techniques in molecular biology including real time RT-PCR analysis, quantification and imaging of gels and storage of bacteria, vectors and cDNA constructs. Each of the modules is staffed by well-trained research assistants who are knowledgeable in the diverse areas of expertise required for each facility. The directorship of each module is composed of established vision scientists who have considerable expertise in the respective research field and who actively function to enhance NEI-supported studies, and develop, encourage and facilitate collaborative efforts in the visual sciences

持续资金由20位视力科学家及其实验室人员的小组提供支持在解剖学/细胞生物学系中安装的两个研究模块将继续提供中央将促进和增强韦恩州和其他参与大学的视力研究的单位缺乏核心支持的设施。这些模块将通过提供此功能来有效地增强当前的研究具有人员和设备的一组科学家，通常不受个人R01或其他类型的支持 NEI赠款，以优先提高Nei资助的研究，并为合作和新的试点项目开发，导致NEI赠款提交。所需的两个模块包括：成像/组织病理学和组织培养/分子。成像/ 组织病理学模块为共聚焦激光扫描，光和电子显微镜的所有方面提供了免疫荧光，使用变质成像软件的图像分析以及显微镜训练和成像软件。数字图像的管理有帮助和培训，包括数字图形制作，动物缝隙摄影，海报和出版物，数码摄影设备使用，高分辨率平板透明度扫描和成像计算机工作站使用情况在模块中。组织培养/分子模块为准备专业培养基，眼细胞的隔离，纯化和表征原代培养，亚文化和已建立的细胞系的传播以及细胞的冷冻保存。协助和培训也是可用于分子生物学的基本技术，包括实时RT-PCR分析，定量以及细菌，载体和cDNA构建体的凝胶成像和储存。每个模块都由训练有素的研究助理组成，他们知识渊博每个设施都需要专业知识领域。每个模块的董事职位由已建立的在各自的研究领域拥有大量专业知识并积极发挥作用的远见科学家为了增强Nei支持的研究，并开发，鼓励和促进视觉上的协作努力科学