Imaging Core

成像核心

• 批准号: 8107465
• 负责人: MICHAEL ROSBASH
• 金额: $ 31.55万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8107465
• 关键词: Biology; Charge; Commit; Communities; Computer software; Drosophila genus; Electrophysiology (science); Equipment; Fees; Funding; Grant; Hour; Human Resources; Image; Individual; Institution; Laboratories; Life; Measurement; Microscope; Program Research Project Grants; Research; Research Personnel; Running; Staging; Structure; Time; Tissues; Universities; Wages; base; cost; experience; fluorophore; instrument; instrumentation; meetings; programs; repaired; time use; tissue processing

We recently received funding to replace an obsolete BioRad 600 confocai microscope with a Leica TCS SP Spectral confocal microscope (S10 RR16708). The day-to-day running of the new confocal is under the direction of Mr. Edward Dougherty, an experienced microscopist. Dougherty's salary is currently paid in part by the Drosophila program project grant (N $44232), partly by Marder's N S 17813, and partly by Biology Department funds. The confocal is available on a fee-for-usage basis for all investigators at Brandeis. The confocal is presently heavily used (60-70 hours/week) as several laboratories (Marder, White, Hall, Griffith) have projects that intensively depend on the confocal, for the study of fixed and processed tissue. There is an urgent need for additional instrumentation and personnel to meet the needs of our user group. A subset of our user" group (Turrigiano [PI], Marder, Griffith, Lisman and Nelson) applied for a Shared Equipment Grant to purchase a Leica Spectral Confocal/Multiphoton microscope, to allow for the study of live tissue combhled with electrophysiology (S10 RR017938). This grant did very well and stands an excellent chance of being funded. The University has committed funds for the installation of this instrument and renovations to accommodate it, but there are currently no institutional funds available for support personnel. Mr. Ed Dougherty will provide oversight for the new confocal and the multiphoton instrument, but the day-to-day running of these new pieces of equipment will require an additional technician. We also lack funds to purchase the electrophysiology equipment needed to fully utilize the potential of the multiphoton set-up. The availability of combined electrophysiology and multiphoton imaging to the user group would allow us to extend our research programs in important new directions. A second gap in our imaging arsenal is the lack of instrumentation and software for time-lapse imaging. A number of the projects of our user group require time-lapse measurements of multiple fluorophores. The set-up costs for this imaging rig (manipulators, stimulators, culture chamber stage) have been prohibitive for any individual, since it would not receive full-time use in a single lab. The addition of equipment and software for this type of analysis would extend existing projects and allow users to investigate these new, highly relevant avenues of research.

我们最近获得了资金，以用Leica TCS SP代替过时的Biorad 600 Cuncocai显微镜 光谱共聚焦显微镜（S10 RR16708）。新共聚焦的日常运行在 经验丰富的显微镜家爱德华·多尔蒂（Edward Dougherty）先生。 Dougherty的薪水目前由果蝇部分支付 计划项目赠款（n $ 44232），部分由Marder's N S 17813，部分由生物学部门基金。这 共焦用于Brandeis的所有调查人员的收费基础上。共聚焦目前已大量使用 （每周60-70小时），因为几个实验室（Marder，White，Hall，Griffith）的项目非常依赖 共焦，用于研究固定组织和加工的组织。 迫切需要额外的仪器和人员来满足我们用户组的需求。一个 我们用户的子集申请了共享设备 授予购买左侧光谱共焦/多光子显微镜，以研究活组织 具有电生理学（S10 RR017938）。这笔赠款表现很好，并且是被资助的绝佳机会。 该大学已承诺为安装本仪器和翻新以适应它的资金，但是 目前尚无支持人员的机构资金。 Ed Dougherty先生将为监督 新的共聚焦和多光仪器，但是这些新设备的日常运行将 需要额外的技术员。我们还缺乏购买充分购买电生理设备的资金 利用多光子设置的潜力。组合电生理学和多光子的可用性 向用户组成像将使我们能够将研究计划扩展到重要的新方向。 我们成像库中的第二个差距是缺乏用于延时成像的仪器和软件。一个 我们用户组的项目数量需要对多个荧光团进行延时测量。设置成本 对于这种成像钻机（操纵器，刺激剂，培养室阶段），对于任何个人来说都是过敏的，因为 它不会在单个实验室中获得全职使用。增加此类分析的设备和软件 将扩展现有项目，并允许用户调查这些新的，高度相关的研究途径。