Admin Core

管理核心

基本信息

批准号：
8034773
负责人：
HOWARD S FOX
金额：
$ 68.54万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-01 至 2011-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8034773
关键词：
AIDS neuropathy Achievement Activity Cycles Anatomy Animal Model Applications Grants Area Arts Auditory Evoked Potentials Benchmarking Biological Assay Biological Models Brain Brain region Calcium Canada Cell Culture Techniques Cells Circadian Rhythms Communities Detection Disease Disease Progression Elements Engineering Environment Evaluation Event Evoked Potentials Faculty Family Felidae Feline Immunodeficiency Virus France Functional disorder Funding Future Genetic Goals HIV-1 Host Defense Host Defense Mechanism Human Image Imagery Immune Immune response In Vitro Infection Institutes Institution International Knowledge Life Manuscripts Measurement Measures Membrane Methodology Methods Mexico Microscopic Modeling Molecular Monkeys Mus Nervous System Physiology Netherlands Neuraxis Neurons Online Systems Pathogenesis Pathology Phenotype Physiological Physiology Preparation Primates Process Property Radio Rattus Recruitment Activity Research Research Personnel Rodent SIV Sensory Services Sleep Slice Slovakia Spectrum Analysis Stream Study models Subfamily lentivirinae Switzerland Synapses Techniques Telemetry Temperature Therapeutic Training Viral Virus Visual Work base chemokine clinical phenotype cytokine defense response extracellular in vitro Assay in vitro Model in vivo innovation interest meetings mouse model neural circuit neuropathology neurophysiology new technology novel strategies patch clamp preclinical study programs receptor receptor expression success

项目摘要

The major goal of the Physiology Core of the Scripps NeuroAIDS Preclinical Studies (SNAPS), CSPAR, is to continue to provide neurophysiological assessment of AIDS-related functional pathology using a variety of established and new animal models with clinical phenotypes relevant for neuroAIDS. The Core services will include continued evaluation of current primate and feline models infected with the analogous lentivirus (SIV and FIV, respectively) as well as assessment of existing and new mouse models representing molecularly engineered phenotypes relevant for HIV-1 infection. The Core will provide a broad spectrum of analyses for the detection of functional disease progression and identification of underlying mechanisms. In addition, the Core will work to recruit new SNAPS collaborators who will further enrich the assessment potential of the Core and its contribution to the neuroAIDS field. The Core is organized into two interacting units that carry out specific scientific analyses. The analyses provide sensitive measures of disease progression, functional deficits and underlying mechanisms. The in vivo analysis unit will carry out studies in mouse, primate and feline models of NeuroAIDS utilizing in vivo techniques including extracellular single unit electrophysiological recording, sensory potential recording and radio telemetry. The in vitro analysis unit will use electrophysiological recordings of brain slices from animal models of NeuroAIDS or cultures prepared from these models. The in vitro unit will also carry out studies of these models using live cell measurement of cytosolic calcium levels and anatomical studies of receptor expression and localization. Important strengths of this SNAPS Core include the expertise and knowledge of the faculty and staff, their long-term working relationships, and their commitment to neuroAIDS research.

Scripps Neuroaids临床前研究（SNAPS）的生理核心的主要目标是CSPAR，是继续使用多种具有与神经辅助相关的临床表型建立的新动物模型。核心服务将包括对当前的灵长类动物和猫科动物模型的持续评估（SIV 和FIV，以及对代表分子的现有和新小鼠模型的评估与HIV-1感染相关的工程表型。核心将为广泛的分析提供检测功能疾病进展和基本机制的鉴定。另外，核心将努力招募新的快照合作者，这些合作者将进一步丰富评估潜力核心及其对神经辅助领域的贡献。核心被组织成两个携带的相互作用单元进行特定的科学分析。分析提供了对疾病进展，功能的敏感度量缺陷和潜在机制。体内分析单元将在小鼠，灵长类动物和利用体内技术（包括细胞外单个单位电生理学）的神经辅助的猫科动物模型记录，感官潜在的记录和射电遥测。体外分析单元将使用来自神经辅助动物模型或由培养物的动物模型的脑切片的电生理记录这些模型。体外单元还将使用实时细胞测量进行研究受体表达和定位的胞质钙水平和解剖学研究。重要的优势该快照核心包括教职员工的专业知识和知识，他们的长期工作人际关系及其对神经辅助研究的承诺。