Tracking Metals From E-cigarettes: From The Coil Into Lung Tissue

追踪电子烟中的金属：从线圈到肺组织

基本信息

批准号：
9788461
负责人：
Markus Hilpert
金额：
$ 19.8万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-20 至 2021-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9788461
关键词：
Activation Analysis Adolescent and Young Adult Aerosols Air Blood Brain Carcinogens Characteristics Chemicals Chromium Chronic Cigarette Control Animal Dangerousness Data Deposition Devices Dose Electronic cigarette Elements Exposure to Female Filament Flavoring Freezing Future Gamma Rays Glass Government Hair Health Health Hazards Heating High School Student Human Individual Inductively Coupled Plasma Mass Spectrometry Inhalation Joints Kidney Laboratories Liquid substance Liver Lung Mainstreaming Manufacturer Name Measurement Measures Metal exposure Metals Mus Neutron Activation Analysis Nickel Nicotine Nitrosamines Pattern Perception Poison Policy Maker Publications Radiation Radiolabeled Reporting Risk Saliva Smoker Smoking Solvents Source Spleen Stream Structure of parenchyma of lung Temperature Testing Time Tissues Tobacco Tobacco use Urine Variant Youth aerosolized base bioaccumulation carcinogenicity cigarette smoke cigarette smoking design electronic cigarette use electronic cigarette user experimental study junior high school lead concentration male metallicity mouse model operation radiotracer response spectral energy tobacco products toxic metal uptake vapor volatile organic compound

项目摘要

PROJECT SUMMARY Electronic cigarettes (ECs) are battery-operated devices that heat a liquid to generate an aerosol vapor inhaled by the user. Despite their increasing popularity, especially among younger users, little is known about the human health effects of EC use. In addition to health concerns about nicotine, flavorings and other chemicals in the liquid, new data from our laboratory, and others, raises concern that ECs may expose users to potentially dangerous metals since a heated wire is used to generate the aerosol, and most coils contain nickel and chromium, known inhalation carcinogens. We hypothesize that EC devices themselves release Ni, Cr and other metals into the heated aerosol and that these potential carcinogens may accumulate in lung tissue and blood. We will test our hypothesis in two ways. First, we will analyze the potential metals contribution of individual components within an EC device by selectively radiolabeling each part individually using Neutron Activation Analysis (NAA) and analyzing its contribution to the metals content of the resultant heated aerosol. Second, we will measure metal concentrations in lung tissue and blood utilizing a mouse model of exposure. We further hypothesize, based on our preliminary studies using ICP-MS analysis of urine and saliva from EC users, and direct measurement of EC aerosol, that variations in coil design, power settings and EC device construction can have significant effects on measured concentrations of Ni, Cr, Pb and other metals. Our Specific Aims include: 1) utilizing NAA to transiently radiolabel various disassembled EC hardware components, followed by reassembly and measurement of the radiation energy spectrum of the collected aerosol to identify specific sources of metal contamination; and 2) mouse exposure experiments to measure and analyze time- and dose-response relationships for Ni, Cr and Pb concentrations in lung and blood following a four week exposure to EC aerosol. Findings from this study are likely to provide crucial and heretofore unavailable information to policy makers to enable them to evaluate potential health risks of toxic metal exposures arising from EC use. We hypothesize that health risks vary depending on operating conditions and specific EC device composition.

项目概要电子烟 (EC) 是一种电池供电的设备，可加热液体以产生可吸入的气溶胶蒸气由用户。尽管它们越来越受欢迎，尤其是在年轻用户中，但人们对它知之甚少。 EC 使用对人类健康的影响。除了尼古丁、调味剂和其他化学物质的健康问题在液体中，来自我们实验室和其他机构的新数据引起了人们的担忧，即 EC 可能会让用户接触到具有潜在危险的金属，因为使用加热丝来产生气溶胶，并且大多数线圈都含有镍和铬，已知的吸入致癌物。我们假设 EC 装置本身释放 Ni、Cr 和其他金属进入加热的气溶胶中，这些潜在的致癌物质可能会积聚在肺组织中，血。我们将以两种方式检验我们的假设。首先，我们将分析潜在的金属贡献通过使用中子分别选择性地对每个部件进行放射性标记，从而实现 EC 装置内的各个部件活化分析 (NAA) 并分析其对所得加热气溶胶金属含量的贡献。其次，我们将利用小鼠暴露模型测量肺组织和血液中的金属浓度。根据我们使用 ICP-MS 对 EC 尿液和唾液进行分析的初步研究，我们进一步假设用户和 EC 气溶胶的直接测量，线圈设计、功率设置和 EC 设备的变化施工会对镍、铬、铅和其他金属的测量浓度产生重大影响。我们的具体目标包括：1) 利用 NAA 对各种拆卸的 EC 硬件进行瞬时放射性标记组件，然后重新组装并测量收集到的辐射能谱用于识别特定金属污染源的气溶胶； 2) 小鼠暴露实验来测量并分析肺和血液中 Ni、Cr 和 Pb 浓度的时间和剂量反应关系暴露于 EC 气溶胶四个星期后。这项研究的结果可能提供重要且重要的信息迄今为止，政策制定者无法获得信息来评估有毒物质的潜在健康风险 EC 使用引起的金属暴露。我们假设健康风险因操作条件而异以及具体的EC装置组成。